IMPACT OF MECHANICAL MYOCARDIAL INJURY PRODUCTS, LPS AND THEIR COMBINATION ON HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS
https://doi.org/10.15789/1563-0625-2014-4-361-366
Abstract
Complicated systemic inflammatory response (SIR) often determines the outcome in patients after cardiac surgery. Systemic endothelial activation plays the most important role in SIR pathogenesis. We have studied the impact of mechanical myocardial injury products, LPS and their combination on human umbilical vein endothelial cells (HUVEC). We have found that HUVEC increase the production of proinflammatory cytokines in response to
cardiomyocyte cytosolic fraction responsible for mechanical injury modeling. 2% cytosolic fraction containing 0.204 ng/mL of Hsp70 was a greater stimulus for endothelial cells to produce IL-6 and IL8 than moderate
endotoxin concentrations.
About the Authors
V. G. Matveeva
Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences, Kemerovo
Russian Federation
Russian Federation
Researcher, Cell Technology Laboratory, Research Institute for Complex Issues of Cardiovascular
Diseases under the Siberian Branch of the Russian Academy of Medical Sciences
A. S. Golovkin
Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences, Kemerovo
Russian Federation
Russian Federation
PhD, MD, Head of department, Research Institute for Complex Issues of Cardiovascular Diseases under
the Siberian Branch of the Russian Academy of Medical Sciences
L. V. Antonova
Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences, Kemerovo
Russian Federation
Russian Federation
PhD, Research fellow, Cell Technology Laboratory, Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences
I. V. Kudryavtsev
Research Institute for Experimental Medicine under the NorthWest Branch of the Russian Academy of Medical Sciences, Saint-Petersburg
Russian Federation
Russian Federation
PhD, Researcher, Research Institute for Experimental Medicine under the NorthWest Branch of the Russian Academy of Medical Sciences
S. V. Ivanov
Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences, Kemerovo
Russian Federation
Russian Federation
PhD, MD, head of laboratory, Research Institute for Complex Issues of Cardiovascular Diseases under the
Siberian Branch of the Russian Academy of Medical Sciences
E. V. Grigoriev
Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences, Kemerovo
Russian Federation
Russian Federation
PhD, Professor, MD, Deputy Director, Research Institute for Complex Issues of Cardiovascular Diseases under the Siberian Branch of the Russian Academy of Medical Sciences
N. V. Artymuk
Kemerovo State Medical Academy, Kemerovo
Russian Federation
Russian Federation
PhD, Prof., Head of the Department of Obstetrics and Gynecology, number 2, Kemerovo State Medical
Academy
A. G. Trishkin
Kemerovo State Medical Academy, Kemerovo
Russian Federation
Russian Federation
PhD, Assistant of the Department of Obstetrics and Gynecology, number 2, Kemerovo State Medical
Academy
E. S. Bikmetova
Kemerovo State Medical Academy, Kemerovo
Russian Federation
Russian Federation
Postgraduate of the Department of Obstetrics and Gynecology, number 2, Kemerovo State Medical
Academy
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Review
For citations:
Matveeva V.G., Golovkin A.S., Antonova L.V., Kudryavtsev I.V., Ivanov S.V., Grigoriev E.V., Artymuk N.V., Trishkin A.G., Bikmetova E.S. IMPACT OF MECHANICAL MYOCARDIAL INJURY PRODUCTS, LPS AND THEIR COMBINATION ON HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS. Medical Immunology (Russia). 2014;16(4):361-366. (In Russ.) https://doi.org/10.15789/1563-0625-2014-4-361-366
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