FEATURES OF CYTOKINE PROFILE IN PATIENTS WITH ATHEROSCLEROSIS
https://doi.org/10.15789/1563-0625-2014-4-323-332
Abstract
Our study included measuring the following biological substances: cytokines (IL-1β, IL-6, IFNγ, TNFα, IL-10, TGF-β1, TGF-β2, IL-2, IL-17); soluble receptors to cytokines (IL-2R, IL-6R, TNFαRI and TNFαRII); total serum cholesterol; indicators of intercellular matrix degradation (metalproteinases-9; tissular inhibitor of MMP-9 1 type (TIMP-1); MMP-9/TIMP 1 and MMP-9/TIMP 2 complexes) in 260 patients with clinically manifestations of coronary and lower limb atherosclerosis. Among the patients with atherosclerosis, we have found increased average levels of IL-6, TGF-β2, TNFα RI, TNFαRII, IL-2R, along with decreased IL-2 and IL-10 concentrations, increase of MMP-9/TIMP-2 ratio in blood serum. The following direct correlations were revealed between MMP-9 and IL-1β; between MMP-9/TIMP-1 complex and IL-1β, IL-2, IL-2R, IL-17, IL-10; TIMP-1 with TNFα, IL-2 and IL-2R. At high levels of the general cholesterol, an increased production of TNFα, IL-17, TGF-β1, IL-2, IL-10, MMP-9, TIMP-1 was detected, as well as decreased amounts of TNFα I and TNFαII soluble receptors in blood serum of these patients.
Direct correlations were established between total blood cholesterol levels and IL-2, IFNγ, MMP- 9, TIMP-1, MMP-9/TIMP-2. However blood cholesterol levels showed reverse correlations with TNFαRI, and TNFαRII.
In the course of this study, we have specified a functional imbalance between pro- and anti-inflammatory mechanisms of immune reaction in clinically expressed atherosclerosis. Significant regulatory interactions have been revealed between cytokine levels, total blood cholesterol and markers of intercellular matrix degradation.
About the Authors
E. P. Turmova
Pacific Medical University, Russian Ministry of Health Care, Vladivostok
Russian Federation
Russian Federation
PhD (Medicine), Teaching Assistant, Human Physiology Department, Pacific Medical University, Russian
Ministry of Health Care
E. V. Markelova
Pacific Medical University, Russian Ministry of Health Care, Vladivostok
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Chief, Human Physiology Department, Pacific Medical University,
Russian Ministry of Health Care
A. A. Silayev
Medical Center of the Far East Federal University, Vladivostok
Russian Federation
Russian Federation
PhD (Medicine), Clinical Anesthesiologist, Chief, Reanimatology Department, Medical Center of the Far
East Federal University
P. A. Lukyanov
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok
Russian Federation
Russian Federation
PhD, MD (Chemistry), Professor, Principal Research Associate, Laboratory of Non-infectious Immunity
Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences
I. V. Chikalovets
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok
Russian Federation
Russian Federation
PhD (Chemistry), Associate Professor, Senior Research Associate, Laboratory of Non-infectious Immunity Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of
Sciences
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For citations:
Turmova E.P., Markelova E.V., Silayev A.A., Lukyanov P.A., Chikalovets I.V. FEATURES OF CYTOKINE PROFILE IN PATIENTS WITH ATHEROSCLEROSIS. Medical Immunology (Russia). 2014;16(4):323-332. (In Russ.) https://doi.org/10.15789/1563-0625-2014-4-323-332
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