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IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS

https://doi.org/10.15789/1563-0625-2013-4-325-334

Abstract

Abstract. Interactions between B1, B2 and bone marrow dendritic cells (DCs) of CBA mice were investigated in the course of immune response to T-independent antigen type 2 (TI 2 Ag) in model system. Splenocytes of Xid-mice CBA/N were used as “fillers”. It was shown that DCs augmented viability of B1 and B2 lymphocytes. Functional activity of the B1 and B2 lymphocytes was determined by ELISPOT method, as relative numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) detectable after four days in culture. An increased immune response to TI 2 Ag in presence of DCs was observed mainly for B2 subpopulation, containing MZ-B lymphocytes as well. Specific effect of DC upon B2 cell subpopulation was confirmed by detection of direct in vitro interactions of DCs with T and B lymphocytes. DCs cultivated with B2 and T lymphocytes, used as fillers, decreased the numbers of AFCs and IFCs, irrespectively of absence or presence of TI-2 Ags. A decrease in AFC and IFC numbers in the cultures with B1 cells was evident in presence of the Ags only. It was shown that the inhibitory effect of DCs depended on their contact interactions. Separate cultures of B1 and B2 cells with DC under transwell conditions resulted into occurence of AFC and IFC in amounts similar to those generated in vitro without DCs.

About the Authors

M. V. Gavrilova
I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Russian Federation

Research Associate, Laboratory of Immunoglobulin Biosynthesis

115533, Nagatinskaya emb., 18, apt 90. Phone: 7 (495) 674-08-42



I. N. Chernyshova
I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Russian Federation

PhD (Medicine), Senior Research Associate, Laboratory of Immunoglobulin Biosynthesis



D. A. Khochenkov
I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Russian Federation

PhD (Biology), Research Associate, Laboratory of Immunoglobulin Biosynthesis



E. V. Sidorova
I.I. Mechnikov Research Institute of Vaccines and Sera, Moscow
Russian Federation

PhD, MD (Biology), Chief, Laboratory of Immunoglobulin Biosynthesis



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Review

For citations:


Gavrilova M.V., Chernyshova I.N., Khochenkov D.A., Sidorova E.V. IN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENS. Medical Immunology (Russia). 2013;15(4):325-334. (In Russ.) https://doi.org/10.15789/1563-0625-2013-4-325-334

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