DETECTION OF AUTO- AND ALLOANTIBODIES AGAINST GRANULOCYTES BY MEANS OF GEL AGGLUTINATION TECHNIQUE
https://doi.org/10.15789/1563-0625-2011-2-3-253-256
Abstract
Аbstract. Granulocyte antigen-specific autoantibodies may be implicated into pathogenesis of autoand alloimmune neutropenia, neonatal alloimmune neutropenia, and acute pulmonary insufficiency occurring post-transfusion. However, detection and identification of clinically significant granulocyte antibodies still represent a technically difficult task. To detect immunoglobulins and/or activated complement on granulocyte surface, we have adapted a Gel Test (ID Micro Typing System, DiaMed, Switzerland) which is commonly applied for diagnostics of RBC-specific antibodies. Our results suggest that the data from this gel test are quite reproducible, and the system may be used for detection of granulocyte-specific antibodies belonging to different immunoglobulin classes. (Med. Immunol., 2011, vol. 13, N 2-3, pp 253-256)
About the Authors
N. V. Mineeva
ФГУРосНИИ Гематологии и трансфузиологии ФМБА России, Санкт-Петербург
Russian Federation
Russian Federation
E. V. Elkhina
ФГУРосНИИ Гематологии и трансфузиологии ФМБА России, Санкт-Петербург
Russian Federation
Russian Federation
N. N. Bodrova
ФГУРосНИИ Гематологии и трансфузиологии ФМБА России, Санкт-Петербург
Russian Federation
Russian Federation
O. A. Zavarzina
ФГУРосНИИ Гематологии и трансфузиологии ФМБА России, Санкт-Петербург
Russian Federation
Russian Federation
References
1. Araki N., Nose Y., Kohsaki M., Mito H., Ito K. Anti-Granulocyte Antibody Screening with Extracted Granulocyte Antigens by a Micro-Mixed Passive Hemagglutination Method // Vox Sang. – 1999. – Vol. 77. – P. 44-51.
2. Bruin M., Dassen A., Pajkrt D., Buddelmeyer L., Kuijpers T., de Haas M.. Primary autoimmune neutrophil antibodies and clinical course // Vox Sang. – 2005. – Vol. 88. – P. 52-59.
3. Evans R., Green F., Lucas G. A review of referrals for neonatal alloimmune neutropenia over a 4-year period (2002-2006) // Vox Sang. – 2006. – Vol. 91, Suppl. 2, 5.
4. Ivankovic Z., Gojceta K., Golubic Cepulic B., Bojanic I., Lukic M., Mazic S., Orlic D. Testing of granulocyte-associated antibodies, part I test features and differences using anti-IgG and anti-IgM reagents of two manufacturers // Vox Sang. – 2006. – Vol. 91, Suppl. 2, 8.
5. Kameoka J., Funato .T., Miura T., Harigae H., Saito J., Yokoyama H., Takahashi S., Yamada M., Sasaki O., Imaizumi M., Takata N., Meguro K., Sasaki1 T. Autoimmune neutropenia in pregnant women causing neonatal neutropenia // British Journal of Haematology. – 2001. – Vol. 114-1. – P. 198.
6. Klein H., Anstee D. Mollisons Blood Transfusion in Clinical Medicine // 11th ed. – Blackwell Publishing, 2005. – 891 p.
7. Lucas G. Granulocyte immunology workshops: Update and progress // Vox Sang. – 2006 – Vol. 91, Suppl. 2. – P. 4-5.
8. Lucas G., Metcalfe P. Platelet and granulocyte glycoprotein polymorphism // Transfusion Medicine. – 2000. – Vol. 10. – P. 157-174.
9. Noonan K., Johnson T. Examining technical aspects of the monoclonal antibody immobilization of granulocyte antigen assay // Vox Sang, – 1997. –Vol. 73. – P. 87-92.
10. Riedler G., Dakin H. Serious Yazards of transfusion underestimates the incidence of transfusion-related acute lung injury in the UK // Vox Sang. – 2004. – Vol. 86. – P. 268.
11. Stroncek D. Granulocyte antigens and antibodies detection // Vox Sang. – 2004. – Vol. 87, Suppl. 1. – P. 91-94.
Review
For citations:
Mineeva N.V., Elkhina E.V., Bodrova N.N., Zavarzina O.A. DETECTION OF AUTO- AND ALLOANTIBODIES AGAINST GRANULOCYTES BY MEANS OF GEL AGGLUTINATION TECHNIQUE. Medical Immunology (Russia). 2011;13(2-3):253-256. (In Russ.) https://doi.org/10.15789/1563-0625-2011-2-3-253-256
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