INFLUENCE OF POLYMORPHIC VARIANTS RS10455025 OF THE TSLP GENE AND RS11811856 OF THE TNFSF4 GENE ON THE RISK OF ATOPIC BRONCHIAL ASTHMA AND ALLERGY IN CHILDREN

Abstract

Introduction. The available experimental data to date indicate the determining role of cytokines in the formation of manifestations of bronchial asthma (BA), which led to the creation of the so-called anti-cytokine approach to the therapy of this disease. [5]

The aim of this study was to determine the contribution of polymorphisms rs10455025 of the TSLP gene and rs11811856 of the TNFSF4 gene with the risk of developing bronchial asthma in children in the Kursk population.

Materials and methods. The study included 999 unrelated individuals, including 526 patients with AD and 473 controls. These functional single nucleotide polymorphisms (SNPs) were genotyped using the MassArray-4 genomic mass spectrometer.

Results. It has been established that the alleles rs11811856G TNFSF4 and rs10455025C TSLP, as well as the genotypes rs11811856-G/G and rs10455025-C/C, are associated with an increased risk of developing bronchial asthma in children. The association of rs11811856 TNFSF4 with an increased risk of developing BA was identified in both boys and girls, while the polymorphism rs10455025 TSLP did not show any associations in the stratified analysis by gender. In the analysis of gene-environment interactions in the overall group, a relationship was established with the risk of asthma depending on the exposure to tobacco smoke and the place of residence of children. The influence of SNP data on the risk of concomitant allergopathology in children with bronchial asthma was established, and the influence of the studied polymorphisms on the indicators of rhinocytogram and spirometry was also established. During the study, it was found that the studied polymorphisms rs10455025 TSLP and rs11811856 TNFSF4 in children with BA are associated with an increased risk of developing food sensitization to banana, rice, duck, and a number of pollen (foxtail, common horsetail, bluegrass, ryegrass, fireweed, ash, barley), and epidermal (human hair) allergens. It has also been found that the studied SNPs reduce the risk of sensitization to house dust, cat hair, rabbit hair, and quinoa pollen.

Conclusion. The present study showed that polymorphisms rs10455025 TSLP and rs11811856 TNFSF4 are significantly associated with the risk of developing asthma and are associated with its clinical features.

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