THE FREQUENCY OF OCCURRENCE OF ANTI-HBC AMONG MEDICAL WORKERS, DEPENDING ON AGE, GENDER, AND OCCUPATIONAL CATEGORIES

Abstract

Despite the availability of specific prophylaxis against viral hepatitis B (HBV), the problem of HBV morbidity among health care workers is not becoming less significant. A latent form of hepatitis (LHB) may play a role in maintaining the epidemic spread of HBV.  The aim of the study was to identify the dependence of the incidence of anti-HBc among employees of medical organizations on age, gender and professional categories.

Blood serum was tested for the presence of anti-HBc using the ELISA method among 1,643 employees of medical organizations (doctors, nurses, orderlies, other personnel). Blood serum samples were examined for the presence of anti-HBc by ELISA using the HepaBest anti-HBc-IgG enzyme-linked immunosorbent assay system manufactured by Vector-Best-Europe JSC. The results were statistically analyzed using Microsoft Office Excel 2010 and Prism9 (GraphPad, USA). For comparison groups of medical workers according to the frequency of occurrence of Anti-HBc, the chi-square criterion was used.

Anti-HBs was detected in 601 people, which amounted to 36.6% of the total study sample, in 1042 people or 63.4% anti-HBs was not detected. Almost half of the paramedical personnel from the study sample were IgG seropositive for the nuclear antigen of the hepatitis B virus (50.6%). There were no special differences in the proportion of anti-HBc detection in men (31.5%) and women (37.0%). In the structure of seropositive anti-HBc in a smaller percentage, anti-HBc is detected in the age category "young age" (24.0%), the largest share falls on the category of "middle age" (45–59 years) - 38.8%, which is associated with the length of service, i.e. with the risk of longer exposure to a biological factor, which is contact with HBV sources.

The frequency of detection of anti-HBc to HBV nuclear antigen along with anti-virus surface protein antibody (anti-HBs) is an indicator of the prevalence of HBV. We consider it possible to recommend conducting anti-HB tests among the personnel of medical organizations before immunization with additional tests (virus DNA) with a positive result in order to exclude medical workers as a source of infection and further monitoring them for timely provision of the necessary medical care, as well as adjusting the volume of immunization against HBV in this professional environment.

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