EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS
https://doi.org/10.15789/1563-0625-2009-2-3-261-264
Abstract
Abstract. Ability of cannabinoid receptor ligands WIN 55.212-2 and anandamid to inhibit synthesis of TNFα and IL-8 was studied in healthy donors and men with allergic disorders. To establish mechanism of action for investigated substances, the selective antagonists of the СВ1-receptor (SR141716A) and for СВ2 - receptor (SR144528) were applied. Studies with whole blood dilutions allowed of approximating in vivo conditions when investigating biological properties of WIN-55.212-2 and anandamid. The synthetic cannabinoids WIN - 55.212-2 and anandamid at a concentration of 3-10 μМ were capable of reducing synthesis of TNFα and IL-8 in lipopolysaccharide-stimulated blood leukocytes, both from healthy donors and subjects with allergic disorders. It was revealed that the antagonist of СВ1-receptor (SR141716A) did not exert a receptor-mediated effect for WIN-55.212-2 and anandamid. Meanwhile, a СВ2-receptor antagonist (SR144528) entirely eliminated completely the blocking effect of anandamid and WIN-55.212-2.
About the Author
E. G. Lobanova
Владивостокский филиал Дальневосточного научного центра физиологии и патологии дыхания, Научно-исследовательский институт медицинской климатологии и восстановительного лечения Сибирского отделения Российская академия медицинских наук, г. Владивосток
Russian Federation
Russian Federation
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Review
For citations:
Lobanova E.G. EFFECTS OF SYNTETIC CANNABINOID RECEPTOR LIGANDS WIN 55.212-2 AND ANANDAMID UPON IN VITRO ACTIVITY OF IMMUNOCOMPETENT CELLS. Medical Immunology (Russia). 2009;11(2-3):261-264. (In Russ.) https://doi.org/10.15789/1563-0625-2009-2-3-261-264
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