Comparative analysis of TREC and KREC concentration levels in children at medical and biological risk (CHD – frequently ill children, children who have been operated for congenital heart defect with combined thymectomy, children – without it) and those conditionally healthy
https://doi.org/10.15789/1563-0625-CAO-16914
Abstract
The purpose of the study was to compare and analyze the levels of KREC (kappa-deleting recombination excision circle) and TREC (T cell receptor excision circle) levels, which indirectly reflect impaired maturation of T and/or B lymphocytes, in children of the medical and biological risk group and the comparison group (patients considered relatively healthy, relative to the population).
The medical and biological risk groups were:
1) 15 children with operated congenital heart defects with combined thymectomy and 9 without it; the average age is 5 months ±4 months and 7 months ±3 months, respectively; and
2) 27 children with relatively frequent morbidity of the respiratory tract (acute respiratory viral infection more than 8 times a year) aged 1.6±1.4 months, among whom the proportion attending a preschool institution is 20 people (74%).
The comparison groups were:
1) 16 relatively healthy children (health group 1) aged 1.7±1.6 years, among whom the proportion of those attending a preschool institution was 13 people (81%); and
2) 48 apparently healthy newborn children, whose average age was 15±12 days.
Quantitative determination of TREC and KREC was carried out using a multiplex test system developed at the Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences (Novosibirsk). The average concentrations of TREC and KREC were determined in apparently healthy newborns. Children with a high incidence of infectious respiratory morbidity (more than 8 cases of acute respiratory viral infection per year) had a significantly higher risk of developing disorders of both T cell and B cell immunity, compared with the healthy population. Children who underwent total thymectomy during surgical treatment of congenital heart defects had a higher risk of developing immunodeficiency conditions, affecting predominantly T cell immunity, in comparison with the group of children operated on with preservation of the thymus gland.
About the Authors
Yu. I. Rovda
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
PhD, MD (Medicine), Professor, Department of Pediatrics and Neonatology, Kemerovo State Medical University.
Kemerovo
Competing Interests:
None
A. V. Vedernikova
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
Alena V. Vedernikova - Senior Lecturer, Department of Pediatrics and Neonatology, Kemerovo State Medical University.
22a Voroshilov St Kemerovo, Kemerovo Region – Kuzbass 650056
Phone: +7 (929) 351-43-82
Competing Interests:
None
A. V. Shabaldin
Kemerovo State Medical University; Research Institute for Complex Problems of Cardiovascular Diseases
Russian Federation
Competing Interests:
Russian Federation
PhD, MD (Medicine), Professor, Department of Outpatient Pediatrics, Propaedeutics of Childhood Diseases and Postgraduate Training Kemerovo State Medical University; Leading Research Associate, Laboratory of Heart Diseases, Department of Cardiac and Vascular Surgery, Research Institute for Complex Problems of Cardiovascular Diseases.
Kemerovo
Competing Interests:
None
M. L. Filipenko
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
PhD (Biology), Leading Research Associate and Head of the Pharmacogenomics Group, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences.
Kemerovo
Competing Interests:
None
A. A. Sinitskaya
Research Institute for Complex Problems of Cardiovascular Diseases
Russian Federation
Competing Interests:
Russian Federation
PhD (Biology), Research Associate, Laboratory of Genomic Medicine, Department of Experimental Medicine, Research Institute for Complex Problems of Cardiovascular Diseases.
Kemerovo
Competing Interests:
None
I. K. Khalivopulo
Research Institute for Complex Problems of Cardiovascular Diseases
Russian Federation
Competing Interests:
Russian Federation
Cardiac Durgeon, Head, Department of Cardiac Surgery No. 2, Research Institute for Complex Problems of Cardiovascular Diseases.
Kemerovo
Competing Interests:
None
E. O. Grishacheva
Research Institute for Complex Problems of Cardiovascular Diseases
Russian Federation
Competing Interests:
Russian Federation
Pediatric Cardiologist, Department of Cardiac Surgery No. 2, Research Institute for Complex Problems of Cardiovascular Diseases.
Kemerovo
Competing Interests:
None
G. A. Otstavnov
Clinical Medical and Sanitary Unit “Energetik”
Russian Federation
Competing Interests:
Russian Federation
Pediatrician, Pediatric Department, Clinical Medical and Sanitary Unit “Energetik”.
Kemerovo
Competing Interests:
None
Ya. V. Shkitin
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
Resident in Pediatrics, Kemerovo State Medical University.
Kemerovo
Competing Interests:
None
N. N. Minyailova
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
PhD, MD (Medicine), Professor, Department of Pediatrics and Neonatology, Kemerovo State Medical University.
Kemerovo
Competing Interests:
None
T. S. Khobotkova
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
Assistant Professor, Department of Pediatrics and Neonatology, Kemerovo State Medical University.
Kemerovo
Competing Interests:
None
V. V. Dadonov
Kemerovo State Medical University
Russian Federation
Competing Interests:
Russian Federation
5th year Student, Pediatric Faculty, Kemerovo State Medical University.
Kemerovo
Competing Interests:
None
References
1. Borisenko D.V., Ivkin A.A., Shukevich D.L. Modern methods of limiting the systemic inflammatory response in the correction of congenital heart defects in children under cardiopulmonary bypass. Kompleksnyye problemy serdechno-sosudistykh zabolevaniy = Complex Problems of Cardiovascular Diseases, 2021, Vol. 10. no. 2, pp. 113-124. (In Russ.)
2. Deryabina S.S., Tuzankina I.A., Shershnev V.N. Determination of reference values for TREC and KREC in dry blood spots of newborns from different gestation ages in Sverdlovsk Region. Meditsinskaya immunologiya = Medical Immunology (Russia), 2018, Vol. 20, no. 1, pp. 85-98. (In Russ.) doi: 10.15789/1563-0625-2018-1-85-98.
3. Chiarini M., Zanotti C., Serana F., Sottini A., Bertoli D., Caimi L., Imberti L. T-cell receptor and K-deleting recombination excision circles in newborn screening of T- and B-cell defects: review of the literature and future challenges. J. Public Health Res., 2013, Vol. 2, no. 1, pp. 9-16.
Review
For citations:
Rovda Yu.I., Vedernikova A.V., Shabaldin A.V., Filipenko M.L., Sinitskaya A.A., Khalivopulo I.K., Grishacheva E.O., Otstavnov G.A., Shkitin Ya.V., Minyailova N.N., Khobotkova T.S., Dadonov V.V. Comparative analysis of TREC and KREC concentration levels in children at medical and biological risk (CHD – frequently ill children, children who have been operated for congenital heart defect with combined thymectomy, children – without it) and those conditionally healthy. Medical Immunology (Russia). 2024;26(5):993-1000. (In Russ.) https://doi.org/10.15789/1563-0625-CAO-16914
Views:
310
Email this article 