EVALUATION OF T CELL IMMUNOLOGICAL MEMORY BY MEANS OF CD45+ EXPRESSION IN HUMANS VACCINATED WITH LIVE REASSORTANT INFLUENZA VACCINE

Abstract. A full-scale development of post-vaccinal immunity is determined by T and B cell-dependent immunological memory which is formed in response to vaccine injection. However, a capacity of existing and newly developed vaccines to stimulate T cell-dependent immunological memory is very poorly studied. Present work is a first attempt of analyzing this issue, with respect to effects of live mucosal influenza vaccine.

The study involved fifty-seven healthy young persons, in whom percentage of T cells with СD45RO+, CD45RA+, CD4+СD45RO+, CD4+CD45RA+, CD8+СD45RO+, and CD8+CD45RA+ phenotypes was determined pre- and post-vaccination.

Following vaccination, increased average T cell levels of all the mentioned phenotypes was revealed in those volunteers who responded to the vaccine with elevated serum antibody titers. Individual data analysis has shown that, among individuals with antibody response, a percentage of persons with CD45RO+ T cells was 35 to 57%, as compared to vaccinated persons without such antibody response (11 to 22%, p < 0.05; p < 0.01). When using CD45RA+ marker, appropriate values among group 1 volunteers varied between 14 and 36%, whereas for group 2, only one case was detected with expression with significantly increased CD8+CD45RA+, expression on T cells post-vaccination.

Hence, a single immunization with live influenza vaccine is followed by significantly increased levels of peripheral CTLs (CD8+) and Th (СD4+) with СD45R0+ (memory cells) and СD45RА+ (naive) phenotypes in sufficient cohort of immunized persons (35 to 60%). Certain relations exist between post-vaccinal accumulation of memory T cells in peripheral blood, and development of a systemic humoral response to vaccination. However, this connection is not absolute, since an increase in these cells post-vaccination occurs in some persons without such a response (up to 25% of immunized individuals). (Med. Immunol., vol. 10, N 6, pp 535-542).

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