Features of cytokine profile in patients with sarcoidosis
https://doi.org/10.15789/1563-0625-FOC-2064
Abstract
Sarcoidosis is an inflammatory disease of unknown etiology with damage to the lungs and other organs characterized by development of necrosis-free epithelioid cell granulomas. Granulomatous inflammation characterized by the activation of different immune systems cells, in particular T lymphocytes, and the cytokines production. Our study was aimed at investigating the characteristics of the cytokine profile of blood plasma in patients with sarcoidosis. We studied peripheral blood plasma samples of patients with sarcoidosis (n = 52). The control blood samples were taken from healthy volunteers (n = 22). The level of 46 cytokines (pg/ml) was determined, as follows: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL- 6, IL-7, IL-9, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IFNα2, IFNγ, TNFα, TNFβ, IL- 1ra, IL-10, EGF, FGF-2, Flt3 Ligand, G-CSF, GM-CSF, PDGF-AA, PDGF-AB / BB, TGFα, VEGF-A, sCD40L, CCL2, CCL3, CCL4, CCL5, CCL7, CCL11, CCL17, CCL20, CCL22, CXCL1, CXCL8, CXCL9, CXCL10, CXCL11, CXCL13, CX3CL1. Significantly higher levels of interleukins and some proinflammatory cytokines were found in the patients with sarcoidosis, i.e., IL-3, 0.70 vs 0.20, p = 0.003; IL-4, 14.37 vs 3.15, p = 0.009; IL-5, 1.06 vs 0.89, p < 0.001; IL-12 (p70), 1.27 vs 0.56, p = 0.028; IL-17A, 1.48 vs 0.43, p < 0.001; IFNα2, 41.79 vs 25.04, p = 0.003; IFNγ, 4.13 vs 1.14, p < 0.001; TNFα, 21.67 vs 6.70, p < 0.001; anti-inflammatory cytokine IL-10, 1.03 vs 0.45, p = 0.019; growth factors: FGF-2, 40.08 vs 30.58, p = 0.008, G-CSF, 24.18 vs 8.21, p = 0.006, and VEGF-A, 42.52 vs 26.76, p = 0.048; chemokines: CCL3, 3.86 vs 1.33, p < 0,001; CCL17, 78.24 vs 26.24, p < 0.001; CCL20, 7.19 vs 5.64, p = 0.021; CCL22, 660.60 vs 405.00, p < 0,001; CXCL9, 4013 vs 1142, p < 0,001; CXCL10, 565.90 vs 196.60, p < 0.001; CXCL11, 230.20 vs 121.10, p = 0.018; CX3CL1, 56.99 vs 5.16, p < 0.001. Peripheral blood chemokine CCL11 levels were significantly lower in patients compared to the group of healthy volunteers: 77.58 vs 124.70, p = 0.022. The features of the cytokine profile in patients with sarcoidosis may indicate their important role in the processes of formation and outcomes of granulomas. These issues require an additional detailed study, comparison with phenotypes, differential course and outcomes of the disease.
About the Authors
N. M. LazarevaRussian Federation
Lazareva Natalia M., Senior Laboratory Assistant, Department of Immunology, First St. Petersburg State I. Pavlov Medical University, St. Petersburg, Russian Federation
197022, St. Petersburg, L. Tolstoy str., 6-8
O. P. Baranova
Russian Federation
Baranova Olga P., PhD (Medicine), Senior Research Associate, Research Institute of Interstitial and Orphan Lung Diseases, Associate Professor, Department of Pulmonology
St. Petersburg
I. V. Kudryavtsev
Russian Federation
Kudryavtsev Igor V., PhD (Biology), Associate Professor, Department of Immunology, First St. Petersburg State I. Pavlov Medical University; Senior Research Associate, Department of Immunology, Institute of Experimental Medicine
St. Petersburg
N. A. Arsentieva
Russian Federation
Arsentieva Natalia A., PhD (Biology), Senior Research Associate, Laboratory of Molecular Immunology
St. Petersburg
N. E. Liubimova
Russian Federation
Liubimova Natalia E., PhD (Biology), Research Associate, Laboratory of Molecular Immunology
St. Petersburg
T. P. Ses’
Russian Federation
Ses’ Tatyana P., PhD, MD (Biology), Professor,Department of Immunology
St. Petersburg
M. M. Ilkovich
Russian Federation
Ilkovich Mikhail M., PhD, MD (Medicine), Professor, Director, Research Institute of Interstitial and Orphan Lung Diseases, Head, Department of Pulmonology
St. Petersburg
Areg A. Totolian
Russian Federation
Totolian Areg A., PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, Head, Department of Immunology, First St. Petersburg State I. Pavlov Medical University; Director, St. Petersburg Pasteur Institute
St. Petersburg
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Review
For citations:
Lazareva N.M., Baranova O.P., Kudryavtsev I.V., Arsentieva N.A., Liubimova N.E., Ses’ T.P., Ilkovich M.M., Totolian A.A. Features of cytokine profile in patients with sarcoidosis. Medical Immunology (Russia). 2020;22(5):993-1002. (In Russ.) https://doi.org/10.15789/1563-0625-FOC-2064