EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
https://doi.org/10.15789/1563-0625-2018-4-535-542
Abstract
Dendritic cells are specialized antigen-presenting cells which are required to involve naive T-lymphocytes into immune response. Dendritic cells are used in numerous research studies, and have also found practical application as a main component of dendritic cell vaccines. In vitro induction of their maturation from monocytes using cytokines is the most widely used method to obtain these cells. In present study, we compared phenotypes of both immature and mature dendritic cells induced from monocytes of adult healthy donors by means of recombinant cytokines provided by different manufacturers. It is shown that recombinant human IL-4 and GM-CSF supplied by sci-store.ru could effectively stimulate in vitro differentiation of human monocytes to immature monocytic dendritic cells. The resulting immature dendritic cells exhibited a typical morphology and phenotype characterized by the absence of CD14 monocyte marker as well as HLA-DR and CD80 costimulatory molecule expression. A fraction of these cells expressed the CD86 costimulatory molecule. The recombinant human IL-6 from sci-store.ru, when used in a mixture of inflammatory mediators (IL-6, IL-1β, TNFα and PGE2), has induced effective terminal differentiation of immature dendritic cells to mature dendritic cells exhibiting typical morphology and phenotype. Virtually all dendritic cells obtained with this reagent were devoid of CD14, and expressed HLA-DR, CD80, CD83 and CD86 at high fluorescence intensity.
About the Authors
V. Yu. Talayev
Nizhny Novgorod Research I.N. Blokhin Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Head, Laboratory of Cellular Immunology,
603950, Nizhny Novgorod, M. Yamskaya str., 71
M. V. Talayeva
Nizhny Novgorod Research I.N. Blokhin Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunology,
Nizhny Novgorod
E. V. Voronina
Nizhny Novgorod Research I.N. Blokhin Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Junior Research Associate, Laboratory of Cellular Immunology,
Nizhny Novgorod
I. E. Zaichenko
Nizhny Novgorod Research I.N. Blokhin Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
PhD (Biology), Leading Research Associate Laboratory of Cellular Immunology,
Nizhny Novgorod
References
1. Талаев В.Ю., Заиченко И.Е., Матвеичев А.В., Талаева М.В., Воронина Е.В. Регуляция апоптоза в процессе созревания дендритных клеток – приглашение на казнь // Иммунология, 2016. Т. 37, № 5. С. 281-291. [Talayev V.Yu., Zaichenko I.E., Matveichev A.V., Talayeva M.V., Voronina E.V. Regulation of apoptosis during dendritic cell maturation – invitation to an execution. Immunologiya = Immunology, 2016, Vol. 37, no. 5, pp. 281-291. (In Russ.)]
2. Colonna M., Trinchirei G., Liu Y.J. Plasmacytoid dendritic cells in immunity. Nat. Immunol., 2004, Vol. 5, no. 12, pp. 1219-1226.
3. Guermonprez P., Valladeau J., Zitvogel L., Thery C., Amigorena S. Antigen presentation and T cell stimulation by dendritic cells. Annu. Rev. Immunol., 2002, Vol. 20, pp. 621-667.
4. Ingersoll M.A., Platt A.M., Potteaux S., Randolph G.J. Monocyte trafficking in acute and chronic inflammation. Trends Immunol., 2011, Vol. 32, no. 10, pp. 470-477.
5. Kalantari T., Kamali-Sarvestani E., Ciric B., Karimi M.H., Kalantari M., Faridar A., Xu H., Rostami A. Generation of immunogenic and tolerogenic clinical-grade dendritic cells. Immunol. Res., 2011, Vol. 51, no. 2-3, pp. 153-160.
6. Kamgang R.K., Ramos I., Duarte L.R., Ghielmetti M., Freudenberg M., Dahinden C., Padovan E. Using distinct molecular signatures of human monocytes and dendritic cells to predict adjuvant activity and pyrogenicity of TLR agonists. Med. Microbiol. Immunol., 2008, Vol. 197, pp. 369-379.
7. Kawai T., Akira S. Toll-like receptor and RIG-I-like receptor signaling. Ann. N.Y. Acad. Sci., 2008, Vol. 1143, pp. 1-20.
8. Merad M., Sathe P., Helft J., Miller J., Mortha A. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting. Annu. Rev. Immunol., 2013, Vol. 31, pp. 563-604.
9. Naik S.H. Dendritic cell protocols. New York: Humana Press, 2010, p. 446.
10. Naik S.H., Sathe P., Park H.Y., Metcalf D., Proietto A.I., Dakic A., Carotta S., O’Keeffe M., Bahlo M., Papenfuss A., Kwak J.Y., Wu L., Shortman K. Development of plasmacytoid and conventional dendritic cell subtypes from single precursor cells derived in vitro and in vivo. Nat. Immunol., 2007, Vol. 8, no. 11, pp. 1217-1226.
11. Onai N., Obata-Onai A., Schmid M.A., Ohteki T., Jarrossay D., Manz M.G. Identification of clonogenic common Flt3+M-CSFR+ plasmacytoid and conventional dendritic cell progenitors in mouse bone marrow. Nat. Immunol., 2007, Vol. 8, no. 11, pp. 1207-1216.
12. Sallusto F., Lanzavecchia A. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J. Exp. Med., 1994, Vol. 179, no. 4, pp. 1109-1118.
13. Steinman R.M. The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol., 1991, Vol. 9, pp. 271-296.
14. Wu L., Dakic A. Development of dendritic cell system. Cell. Mol. Immunol., 2004, Vol. 1, no. 2, pp. 112-118.
15. Wu L., Liu Y-J. Development of dendritic-cell lineages. Immunity, 2007, Vol. 26, pp. 741-750.
Review
For citations:
Talayev V.Yu., Talayeva M.V., Voronina E.V., Zaichenko I.E. EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS. Medical Immunology (Russia). 2018;20(4):535-542. (In Russ.) https://doi.org/10.15789/1563-0625-2018-4-535-542
Views:
1150
Email this article 