FREQUENCY OF DEVIATIONS FROM NORMAL VALUES IN THE BLOOD SUBPOPULATIONS’ PROFILE IN THE PATIENTS WITH CENTRAL CORNEAL ULCERS
https://doi.org/10.15789/1563-0625-2018-2-263-270
Abstract
This article presents a detailed description of shifts in subsets of blood lymphocyte subpopulations associated with development of the corneal ulcerative lesions as well as during penetrating keratoplasty.
We have revealed some general changes typical for patients with corneal ulcer and patients with keratoplasty, reflecting inflammatory activity, i.e., a significant increase in the numbers of lymphocytes (CD45+), relative and absolute contents of the T-cell population (CD3+), CD3+ CD4+ subpopulation, and B-lymphocytes (CD19+), as compared with the control group. A statistically significant increase in natural killer cells (СD16+СD56+) proved to be a distinctive feature of the group with keratoplasty.
When analyzing mean values of CD4+/CD8+ cells from the patients with corneal ulcer, there was no statistically significant difference between the clinical groups studied. However, individual comparison of nature and frequency of CD4+/CD8+ shifts among the patients with «high» index allowed qualitative separation of the third group (severe corneal ulcer): a severe imbalance of main T-cell subpopulations was detected 2.4 times significantly more often than in the group of patients with mild forms of corneal ulcers.
The obtained data are important for understanding pathogenesis of centrally localized corneal ulcer, and should be taken into account when predicting and developing a differentiated approach to the treatment of the disease.
About the Authors
I. G. Kulikova
Moscow Helmholtz Research Institute of Eye Diseases
Russian Federation
Russian Federation
Senior Research Associate, Department of Immunology and Virology
105062, Russian Federation, Moscow, SadovayaChernogryazskaya str., 14/19. Phone: 7 (926) 386-40-29
N. V. Balatskaya
Moscow Helmholtz Research Institute of Eye Diseases
Russian Federation
PhD (Biology), Leading Research Associate, Department of Immunology and Virology
Russian Federation
PhD (Biology), Leading Research Associate, Department of Immunology and Virology
L. A. Kovaleva
Moscow Helmholtz Research Institute of Eye Diseases
Russian Federation
Research Associate, Department of Infectious and Allergic Eye Diseases
Russian Federation
Research Associate, Department of Infectious and Allergic Eye Diseases
P. V. Makarov
Moscow Helmholtz Research Institute of Eye Diseases
Russian Federation
PhD, MD (Medicine), Leading Research Associate, Department of Traumatology
Russian Federation
PhD, MD (Medicine), Leading Research Associate, Department of Traumatology
References
1. Алексеева Л. А., Железникова Г.Ф., Жирков А.А., Скрипченко Н.В., Вильниц А.А., Монахова Н.Е., Бессонова Т.В. Субпопуляции лимфоцитов и цитокины в крови и цереброспинальной жидкости при вирусных и бактериальных менингитах // Инфекция и иммунитет, 2016. Т. 6, № 1. С. 33-44. [Alekseeva L.A., Zheleznikova G.F., Zhirkov A.A., Skripchenko N.V., Vilnits A.A., Monakhova N.E., Bessonova T.V. Limphocyte subsets and cytokines in blood and cerebrospinal fluid in children with viral fnd bakterial meningitis. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2016, Vol. 6, no. 1, pp. 33-44. (In Russ.)] doi: 10.15789/2220- 7619-2016-1-33-44.
2. Арестова Н.Н., Бржеский В.В., Вахова Е.С., Яни Е.В. Клиника, диагностика и консервативная терапия бактериальных язв роговицы // Федеральные клинические рекомендации. М., 2014. С. 16. [Arestova N.N., Brzhesky V.V., Vakhova E.S., Yani E.V. Clinic, diagnosis and conservative therapy of bacterial corneal ulcers. Federal clinical guidelines]. Moscow, 2014, p. 16.
3. Ковалева Л.А., Слепова О.С., Куликова И.Г., Миронкова Е.А. Роль аутоиммунного компонента при центральных язвах роговицы // Российский офтальмологический журнал, 2013. № 2. С. 29-31. [Kovaleva L.A., Slepova O.S., Kulikova I.G., Mironkova E.A. The role of the autoimmune component in central corneal ulcers. Rossiyskiy oftalmologicheskiy zhurnal = Russian Journal of Ophthalmology, 2013, no. 2, pp. 29-31. (In Russ.)]
4. Копаева В.Г. Глазные болезни. М.: Медицина, 2002. С. 200-244. [Kopaeva V.G. Eye diseases]. Moscow: Medicine, 2002, pp. 200-244.
5. Кудрявцев И.В., Захарова Г.А., Гапонова Т.В. Исследование основных субпопуляций Т-хелперов и цитотоксических Т-клеток при ревматоидном артрите // Российский иммунологический журнал, 2014. Т. 8 (17), № 2 (1). С. 89-92. [Kudryavtsev I.V., Zakharova G.A., Gaponova T.V. Investigation of the main subpopulations of T-helpers and cytotoxic T cells in rheumatoid arthritis. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2014, Vol. 8 (17), no. 2 (1), pp. 89-92. (In Russ.)]
6. Куликова И.Г., Слепова О.С., Макаров П.В., Ковалева Л.А., Кугушева А.Э. Роль (суб)популяционного дисбаланса лимфоцитов крови в развитии системной органоспецифической сенсибилизации при воспалительной и посттравматической патологии глаз // Российский офтальмологический журнал, 2013. Т. 6, № 4. С. 71-75. [Kulikova I.G., Slepova O.S., Mironkova E.A., Makarov P.V., Kugusheva A.E., Kovaleva L.A. The role of (sub) populational imbalance of blood lymphocytes in development of systemic organ-specific sensitization in inflammatory and posttraumatic eye diseases. Rossiyskiy oftalmologicheskiy zhurnal = Russian Journal of Ophthalmology, 2013, Vol. 6, no. 4, pp. 71-75. (In Russ.)]
7. Майчук Ю.Ф. Терапевтические алгоритмы при инфекционных язвах роговицы // Вестник офтальмологии, 2000. № 3. С. 35-37. [Maychuk Yu.F. Therapeutic algorithms for corneal infectious ulcers. Vestnik oftalmologii = Bulletin of Ophthalmology, 2000, no. 3, pp. 35-37. (In Russ.)]
8. Ченцова Е.В., Вериго Е.Н., Хазамова А.И., Яни Е.В. Современные аспекты лечения язвенных поражений роговицы // Евразийский союз ученых, 2016. № 2-2 (23). С. 99-105. [Chentsova E.V., Verigo E.N., Khazamova A.I., Yani E.V. Modern aspects of the treatment of corneal ulcerations. Evraziyskiy soyuz uchenykh = Eurasian Union of Scientists, 2016, no. 2-2 (23), pp. 99-105. (In Russ.)]
9. Ярилин А.А. Иммунология. М.: ГЕОТАР-Медиа, 2010. С. 314-332, 588-624. [Yarilin A.A. Immunology]. Moscow: GEOTAR-Media, 2010, pp. 314-332, 588-624.
10. Armengol M.P., Sabater L., Fernandez M. Influx of recent thymic emigrants into autoimmune thyroid disease glands in humans. Clinical and Experimental Immunology, 2008, Vol. 153, pp. 338-350.
11. Kafkala C., Choi J., Zafirakis P., Baltatzis S., Livir-Rallatos C., Rojas B., Foster C.S. Mooren ulcer: an immunopathologic study. Cornea, 2006, Vol. 25, no. 6, pp. 667-673.
12. Liu F., Duan X., Wan Z., Zang H., You S., Yang R., Liu H., Li D., Li J., Zhang Y., Xin S. Lower number and decreased function of natural killer cells in hepatitis B virus related acute-on-chronic liver failure. Clin. Res. Hepatol. Gastroenterol., 2016, Vol. 40, no. 5, pp. 605-613.
13. Liu Q., Smith C.W., Zhang W., Burns A.R., Li Z. NK cells modulate the inflammatory response to corneal epithelial abrasion and thereby support wound healing. Аm. J. Pathol., 2012, Vol. 181, no. 2, pp. 452-462.
14. López-Botet M., Vilches C., Redondo-Pachón D., Muntasell A., Pupuleku A., Yélamos J., Pascual J., Crespo M. Dual role of natural killer cells on graft rejection and control of cytomegalovirus infection in renal transplantation. Front. Immunol., 2017, no. 8, p. 166.
15. Nascimbeni M., Shin E.C., Chiriboga L., Kleiner D.E., Rehermann B. Peripheral CD4(+)CD8(+) T cells are differentiated effector memory cells with antiviral functions. Blood, 2004, Vol. 104, no. 2, pp. 478-486.
16. Nurozler A.B., Salvarli S., Budak K. Results of therapeutic penetrating keratoplasty. Jpn. J. Ophthalmol., 2004, Vol. 48, no. 4, pp. 368-371.
17. Parel Y., Aurrand-Lions M., Scheja A. Presence of CD4+CD8+ double-positive T cells with very high interleukin-4 production potential in lesional skin of patients with systemic sclerosis. Arthritis. Rheum., 2007, Vol. 56, pp. 3459-3467.
18. Schwartzkopff J., Schlereth S.L., Berger M., Bredow L., Birnbaum F., Böhringer D., Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats. MolVis, 2010, no. 16, pp. 1928-1935.
Review
For citations:
Kulikova I.G., Balatskaya N.V., Kovaleva L.A., Makarov P.V. FREQUENCY OF DEVIATIONS FROM NORMAL VALUES IN THE BLOOD SUBPOPULATIONS’ PROFILE IN THE PATIENTS WITH CENTRAL CORNEAL ULCERS. Medical Immunology (Russia). 2018;20(2):263-270. (In Russ.) https://doi.org/10.15789/1563-0625-2018-2-263-270
Views:
837
Email this article 