PRELIMINARY CLINICAL RESULTS WITH LIPOASPIRATE STROMAL VASCULAR CELL FRACTION IN TREATMENT OF PATIENTS WITH KNEE OSTEOARTHRITIS
https://doi.org/10.15789/1563-0625-2017-6-779-788
Abstract
The paper presents results of clinical application of autologous stromal-vascular fraction (SVF) cells in patients with degenerative osteoarthritis (OA) of the knee, grade II and III (Kellgren–Lawrence scale).
We recruited six patients with knee OA (3 men and 3 women; median age 64 years) with mean disease duration of 7 years. All the patients were administered a single intra-articular injection of autologous nucleated SVF cells at an average dose of 16.8±0.9 × 106 per joint (a total of 11 joints). The patients did not experience any serious side effects (allergic, toxic or inflammatory) related to the knee injection. Patient surveys at 1 month after SVF administration revealed a decrease in the severity of pain, as measured by a visual analog scale (VAS) and a specialized 100-point scale KOOS (subscale "pain") (p < 0.05 on both scales). Moreover, the patients reported improvement in the joint functions and quality of life related to affected joints on a KOOS scale (p < 0.05). These positive clinical changes persisted during 6 month follow up. Significant improvements were noted in ultrasound findings, with increased thickness of the cartilage layer at 3 months (in 73% of cases) and at 6 months (in 82%). Our pilot study demonstrated the safety and tolerability of intra-articular injection of autologous SVF cells in patients with moderate to severe OA. The results obtained also indicate a significant antiinflammatory effect of autologous adipose tissue SVF cells, which is manifested at the early stages of cell therapy. Our further investigations will be focused on exploring the SVF stimulatory effects on regeneration of damaged joints.
About the Authors
E. Ya. Shevela
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Russian Federation
PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
630099, Russian Federation, Novosibirsk, Yadrintsevskaya str., 14
N. A. Nitsa
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Physician (Therapy), Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Russian Federation
Physician (Therapy), Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
N. M. Starostina
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Honored Doctor of Russian Federation, PhD (Medicine), Head, Immunology Department, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Russian Federation
Honored Doctor of Russian Federation, PhD (Medicine), Head, Immunology Department, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
S. I. Baranov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Doctor of Ultrasonic Diagnostics, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Russian Federation
Doctor of Ultrasonic Diagnostics, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Yu. A. Kozhevnikov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD (Medicine), Head, Surgical Department, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD (Medicine), Head, Surgical Department, Clinic of Immunopathology, Research Institute of Fundamental and Clinical Immunology
N. D. Popova
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Clinical Intern (2nd year of study), Research Institute of Fundamental and Clinical Immunology
Russian Federation
Clinical Intern (2nd year of study), Research Institute of Fundamental and Clinical Immunology
E. V. Batorov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
A. A. Ostanin
Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD, MD (Medicine), Professor, Main Research Associate, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD, MD (Medicine), Professor, Main Research Associate, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
E. R. Chernykh
Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Sciences, Head, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
Russian Federation
PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Sciences, Head, Laboratory of Cellular Immunotherapy, Research Institute of Fundamental and Clinical Immunology
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Review
For citations:
Shevela E.Ya., Nitsa N.A., Starostina N.M., Baranov S.I., Kozhevnikov Yu.A., Popova N.D., Batorov E.V., Ostanin A.A., Chernykh E.R. PRELIMINARY CLINICAL RESULTS WITH LIPOASPIRATE STROMAL VASCULAR CELL FRACTION IN TREATMENT OF PATIENTS WITH KNEE OSTEOARTHRITIS. Medical Immunology (Russia). 2017;19(6):779-788. (In Russ.) https://doi.org/10.15789/1563-0625-2017-6-779-788
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