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FEATURES OF HLA-DRB1 AND DQB1 ALLELES DISTRIBUTION IN CHILDREN WITH GENERALIZED TUBERCULOSIS

https://doi.org/10.15789/1563-0625-2017-6-755-762

Abstract

So far, there is no clear understanding for the reasons causing tuberculosis generalization in children. Development of generalized infection could be also determined by some features of immune response which may depend on genetic markers. The aim of our work was to study the features of HLA-DRB1 and HLADQB1 allele distribution in children with generalized tuberculosis (gTBC). Materials and methods: we have observed 40 children at the age of 1 to 15 years suffering from gTBC. After performing a standard phthysiatric examination, including molecular-genetic diagnostics of surgical material, multispiral CT, immunological testing, as well as HLA-DRB1 and DQB1 alleles. We have studied distribution of the HLA-DRB1 and DQB1 alleles comparing it with a group of healthy donors (n = 100).
The results did not show significant differences in HLA-DQB1 allele distribution between the gTBC patients and comparison group. Meanwhile, when comparing genotypes for HLA-DRB1 loci, we have revealed a significantly lower prevalence of the *01 allele of HLA-DRB1 (12.5% versus 31.0%; χ2 = 4.08; р < 0.05), which could be considered a predisposing factor, as well as a higher incidence of *14 allele (7.5% against 1.0%; χ2 = 3.67, р < 0.05) of HLA-DRB1 gene, thus suggesting its protective role.
Conclusions: we did not find any significant differences for HLA-DQB1 distribution between pediatric patients with generalized tuberculosis and healthy donors, whereas * 01 allele of HLA-DRB1 may predispose for gTBC, and the *14 allele could be protective in evolving generalized tuberculosis in children.

About the Authors

A. A. Starshinova
St. Petersburg Research Institute of Phthisiopulmonology; St. Petersburg State University
Russian Federation

PhD, MD (Medicine), Leading Research Associate, St. Petersburg Research Institute of Phthisiopulmonology; St. Petersburg State University

191036, Russia, St. Petersburg, Ligovsky ave, 2-4.



A. S. Berkos
Russian Research Institute of Hematology and Transfusiology
Russian Federation
PhD (Medicine), Head, Tissue Typing Laboratory of the Republican Center Immunological Tissue Typing, Russian Research Institute of Hematology and Transfusiology


V. A. Vatutina
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
Clinical Phthisiatrist, Department for Pediatric Patients Patients with Osteoarticular Tuberculosis, St. Petersburg Research Institute of Phthisiopulmonology


Yu. E. Ovchinnikova
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
PhD (Medicine), Senior Research Associate, St. Petersburg Research Institute of Phthisiopulmonology


Yu. V. Sokolova
Russian Research Institute of Hematology and Transfusiology
Russian Federation
PhD (Biology), Senior Research Associate, Russian Research Institute of Hematology and Transfusiology


I. F. Dovgalyuk
St. Petersburg Research Institute of Phthisiopulmonology
Russian Federation
PhD, MD (Medicine), Professor, Chief of Thye «Children Phthisiatry» Direction, St. Petersburg Research Institute of Phthisiopulmonology


L. N. Bubnova
Russian Research Institute of Hematology and Transfusiology; Pavlov First St. Petersburg State Medical University
Russian Federation
Honored Worker of Science of the Russian Federation, PhD, MD (Medicine), Professor, Head, Laboratory of Immunohematology and Russian Center of Tissue Typing, Russian Research Institute of Hematology and Transfusiology; Pavlov First St. Petersburg State Medical University, Department оf Immunology


P. K. Yablonskiy
St. Petersburg Research Institute of Phthisiopulmonology; St. Petersburg State Universit
Russian Federation
PhD, MD (Medicine), Professor, Director, St. Petersburg Research Institute of Phthisiopulmonology; Dean, Faculty of Medicine, St. Petersburg State University


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Review

For citations:


Starshinova A.A., Berkos A.S., Vatutina V.A., Ovchinnikova Yu.E., Sokolova Yu.V., Dovgalyuk I.F., Bubnova L.N., Yablonskiy P.K. FEATURES OF HLA-DRB1 AND DQB1 ALLELES DISTRIBUTION IN CHILDREN WITH GENERALIZED TUBERCULOSIS. Medical Immunology (Russia). 2017;19(6):755-762. (In Russ.) https://doi.org/10.15789/1563-0625-2017-6-755-762

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