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Медицинская иммунология

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МНОГОЦВЕТНЫЙ ЦИТОМЕТРИЧЕСКИЙ АНАЛИЗ. ИДЕНТИФИКАЦИЯ Т-КЛЕТОК И ИХ СУБПОПУЛЯЦИЙ ПО ЭКСПРЕССИИ αβ-ТCR И γδ-ТCR

https://doi.org/10.15789/1563-0625-2008-2-3-115-124

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Аннотация

Резюме. Т-лимфоциты играют важную роль в элиминации опухолевых клеток в реакциях трансплантат против хозяина и хозяин против трансплантата, гиперчуствительности замедленного типа и других реакциях организма, направленных на поддержание гомеостаза. Помимо молекул CD3 антиген-специфический T-клеточный рецептор является другим пан-T-клеточным маркером. Существует два отличных типа TcR – αβ-TcR и γδ-TcR, которые различаются в онтогенезе и функциональных свойствах. γδ-T-клетки играют значительную роль в защите организма от различных типов инфекций, и знание об их количественном составе должно быть неотъемлемой частью анализа иммунного статуса пациентов. Для этих целей следует использовать многоцветный анализ и следующие комбинации моноклональных антител: CD3/CD4/CD8/CD45 и αβ-TcR/γδ-TcR/CD3/CD45. Применение многоцветного окрашивания и многоэтапного гейтирования позволяют провести многопараметрический анализ T-клеток периферической крови с высокой точностью и достоверностью. Данный подход значительно облегчает интерпретацию полученных результатов и позволяет судить о функционировании иммунной системы больных при разнообразных патологических состояниях.

Об авторах

С. В. Хайдуков
Институт биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, Москва
Россия
117997, Москва, ул. Миклухо-Маклая, 16/10


А. В. Зурочка
ГОУ ВПО Челябинская государственная медицинская академия Росздрава, г. Челябинск
Россия


В. А. Черешнев
Институт иммунологии и физиологии УРО РАН, г. Екатеринбург
Россия


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Для цитирования:


Хайдуков С.В., Зурочка А.В., Черешнев В.А. МНОГОЦВЕТНЫЙ ЦИТОМЕТРИЧЕСКИЙ АНАЛИЗ. ИДЕНТИФИКАЦИЯ Т-КЛЕТОК И ИХ СУБПОПУЛЯЦИЙ ПО ЭКСПРЕССИИ αβ-ТCR И γδ-ТCR. Медицинская иммунология. 2008;10(2-3):115-124. https://doi.org/10.15789/1563-0625-2008-2-3-115-124

For citation:


Khaidukov S.V., Zurochka A.V., Chereshnev V.A. MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF T LYMPHOCYTES AND THEIR SUBSETS. Medical Immunology (Russia). 2008;10(2-3):115-124. (In Russ.) https://doi.org/10.15789/1563-0625-2008-2-3-115-124

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