mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2015-2-119-126mimmun-829Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭКСПРЕССИЯ ПАТТЕРН-РАСПОЗНАЮЩИХ РЕЦЕПТОРОВ И ТРАНСКРИПЦИОННЫХ РЕГУЛЯТОРОВ ДИФФЕРЕНЦИРОВКИ Т-ХЕЛПЕРОВ ЛИМФОЦИТАМИ КИШЕЧНИКА ПРИ ЭКСПЕРИМЕНТАЛЬНОМ ИЛЕИТЕ И ПРИ ВВЕДЕНИИ СИМВАСТАТИНА И АНТАГОНИСТА РЕЦЕПТОРОВ ИНТЕРЛЕЙКИНА-1EXPRESSION OF PATTERN-RECOGNIZING RECEPTORS AND TRANSCRIPTIONAL REGULATORS OF T HELPER CELL DIFFERENTIATION BY INTESTINAL LYMPHOCYTES IN EXPERIMENTAL ILEITIS AND UPON ADMINISTRATION OF SIMVASTATIN AND INTERLEUKIN-1 RECEPTOR ANTAGONISTЖеребятьевА. С.ZherebiatievA. S.

ассистент кафедры микробиологии, вирусологии и иммунологии, Запорожский государственный медицинский университет, г. Запорожье, Украина 

Assistant Professor, Department of Microbiology, Virology, and Immunology, Zaporozhye State Medical University, Zaporozhye, Ukraine 

Gerya2009@yandex.ruКамышныйА. М.KamyshnyiA. M.

д.м.н., доцент, заведующий кафедрой микробиологии, вирусологии и иммунологии, Запорожский государственный медицинский университет, г. Запорожье, Украина 

PhD, MD (Medicine), Associate Professor, Head, Department of Microbiology, Virology, and Immunology, Zaporozhye State Medical University, Zaporozhye, Ukraine 

Gerya2009@yandex.ruКамышнаяВ. А.KamyshnayaV. A.

к.м.н., старший преподаватель кафедры микробиологии, вирусологии и иммунологии, Запорожский государственный медицинский университет, г. Запорожье, Украина 

PhD (Medicine), Senior Lecturer, Department of Microbiology, Virology, and Immunology, Zaporozhye State Medical University, Zaporozhye, Ukraine 

Gerya2009@yandex.ruЗапорожский государственный медицинский университет 69035, Украина, г. Запорожье, пр. Маяковского, 26.РоссияZaporozhye State Medical University 69035, Ukraine, Zaporozhye, Mayakovsky pr., 26.Russian Federation201526042015172119126Copyright © Жеребятьев А.С., Камышный А.М., Камышная В.А., 20152015Жеребятьев А.С., Камышный А.М., Камышная В.А.Zherebiatiev A.S., Kamyshnyi A.M., Kamyshnaya V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/829

Патогенез воспалительных заболеваний кишечника является сложным и мультифакторным. Т-хелперы являются компонентами адаптивного иммунного ответа, тогда как Toll-подобные рецепторы, NOD-подобные рецепторы, RIG-I-подобные рецепторы участвуют в сохранении гомеостаза между слизистой оболочкой и синантропными микроорганизмами. 

Мы изучили возможность применения симвастатина и антагониста рецепторов интерлейкина-1 для коррекции экспериментального илеита у крыс с акцентом на исследование экспрессии TLR2, TLR4, NOD2, RIG-I и транскрипционных факторов T-bet, GATA-3, RORγt и FoxP3 лимфоцитами тонкой кишки. 

Эксперимент проводили на самцах крыс линии Вистар в возрасте 5-7 месяцев. Иммунопозитивные лимфоциты были идентифицированы с помощью метода прямой и непрямой иммунофлюоресценции с использованием моноклональных антител крысы. 

Развитие острого и хронического илеита сопровождалось однонаправленным увеличением количества TLR2+ лимфоцитов и снижением общего количества TLR4+ и FoxP3+ лимфоцитов в лимфоидных структурах подвздошной кишки. Введение симвастатина и АРИЛ-1 экспериментальным животным при развитии экспериментальной патологии сопровождалось снижением количества RORγt+ и T-bet+ лимфоцитов и увеличением общего числа FoxP3+ лимфоцитов. 

Симвастатин и антагонист рецепторов интерлейкина-1, кажется, благоприятно влияет на исход и течение индометацин-индуцированного илеита через модулирование экспрессии образ-распознающих рецепторов на лимфоцитах и баланса между различными субпопуляциями Т-хелперов тонкой кишки. 

Pathogenesis of inflammatory bowel disease is complicated and multifactorial. T helper cells represent components of adaptive immune response, whereas Toll-like receptors, NOD-likereceptors, RIG-I-like receptors are involved in maintenance of mucosal, as well as commensal homeostasis. Aim of study was to evaluate the ability of Simvastatin and IL-1 receptor antagonist to modify the course of experimental ileitis in rats, with a focus on expression of TLR2, TLR4, NOD2, RIG-I, like as T-bet, GATA-3, RORγt, and FoxP3 transcription factors by resident lymphocytes in the small intestine. 

Experiments were carried out with male Wistar rats (5 to 7 months old). The immunopositive lymphocytes were determined by means of direct and indirect immunofluorescence technique, using monoclonal rat antibodies. 

Development of acute and chronic ileitis was associated with unidirectional tendency for increase of TLR2+ lymphocyte numbers, and decrease in total TLR4+ and FoxP3+ lymphocyte counts in lymphoid structures of ileum. Treatment of experimental animals with Simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of RORγt+ and T-bet+ lymphocytes, along with increasing total numbers of FoxP3+ lymphocytes. 

Simvastatin and antagonist of IL-1 receptors seem to exert a beneficial effect upon the course and outcomes in the indomethacin-induced rat ileitis model, via changing expression of pattern-recognizing receptors on lymphocytes and modulation of balance between different T helper cell subsets of intestinal tissues.

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The authors declare that there are no conflicts of interest present.