References

mimmun

Медицинская иммунология

Medical Immunology (Russia)

1563-06252313-741X

SPb RAACI

10.15789/1563-0625-2014-4-353-360

mimmun-701

Research Article

ОРИГИНАЛЬНЫЕ СТАТЬИ

ORIGINAL ARTICLES

РЕГУЛЯТОРНЫЕ Т-КЛЕТКИ У ДЕТЕЙ С АУТОИММУННЫМИ ЗАБОЛЕВАНИЯМИ

REGULATORY T-CELLS IN CHILDREN WITH AUTOIMMUNE DISEASES

Пашнина

И. А.

Pashnina

I. A.

к.б.н, зав. лабораторией иммунологии Отдела клинической иммунологии Областной детскойклинической больницы №1, науч. сотр. лаборатории иммунопатофизиологии Института иммунологиии физиологии УрО РАН

PhD, chef of Immunological laboratory of Immunological department, Regional Children's Clinical Hospital №1; research worker of Immunopathology laboratory, Institute of Immunology and Physiology, Urals Branch of the Russian Academy of Science

irina_pashnina@list.ru

ГБУЗ СО Областная детская клиническая больница №1, г. Екатеринбург ФГБУН Институт иммунологии и физиологии УрО РАН, г. ЕкатеринбургРоссияRegional Children's Clinical Hospital №1, Yekaterinburg Institute of Immunology and Physiology, Urals Branch of the Russian Academy of Science, YekaterinburgRussian Federation

2014

01082014

164353360

2014

Пашнина И.А.

Pashnina I.A.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.mimmun.ru/mimmun/article/view/701

Обследованы дети и подростки 7-17 лет: с ювенильным артритом (n=99), с реактивным артритом (n=21), с ювенильной склеродермией (n=16), с системной красной волчанкой (n=14), условно здоровые (n=32). Методом проточной цитометрии выявлено снижение количества регуляторных Т-лимфоцитов (CD4+CD25+CD127low/neg) у детей с реактивным артритом, ювенильными артритами, ювенильной склеродермией по сравнению со здоровыми детьми, у больных с системной красной волчанкой число Treg не отличалось от контрольного. Снижение количества регуляторных Т-клеток в большинстве обследованных групп больных указывает на участие этих лимфоцитов в развитии аутоиммунной патологии у детей. Остается открытым вопрос о том, какие причины способствуют поддержанию нормального количества Treg при СКВ, в отличие от других аутоиммунных заболеваний. Выявлена отрицательная корреляционная взаимосвязь между количеством Treg и индексом SLEDAI при СКВ, положительная взаимосвязь - между количеством Treg и числом пораженных суставов при РеА. Количество CD3+CD25+ и CD3+CD4+CD25+лимфоцитов у обследованных детей было достоверно взаимосвязано между собой, а также с числом Treg, что обуславливает нецелесообразность определения Т-лимфоцитов и Т-хелперов, экспрессирующих CD25, в качестве самостоятельных параметров.

Children and teenagers aged 10-17 years old with juvenile arthritis (n=99), with reactive arthritis (n=21), with systemic sclerosis (n=16), with systemic lupus erythematosus (n=14) and conditionally healthy (n=32) are investigated. It’s revealed by the method of flow cytometry that quantity of regulatory T-cells (CD3+CD4+CD25+CD127low/neg) in children with juvenile arthritis, with reactive arthritis and with systemicsclerosis was lower than in the control group. The amount of Treg in children with systemic lupus erythematosus was the same to the control level. The decrease of Treg number in most of investigated groups indicates that these cells are involved in the pathogenesis of an autoimmune diseases in children. It’s remaining unknown what’s the reason of normal Treg content in patients with systemic lupus erythematosus in contrast with other autoimmune diseases. There were positive correlation between the percentage of Treg and the amount ofdamaged joints in children with reactive arthritis and negative correlation between the amount of Treg and SLEDAI in children with systemic lupus erythematosus. The significant correlations between the numbers of CD3+CD25+, CD3+CD4+CD25+ and Treg were revealed, so there isn’t any reasonability of estimation of CD3+ and CD4+cells which are expressed CD25 as separate parameters.

регуляторные Т-клеткиаутоиммунные заболеваниядети

regulatory T-cellsjuvenile arthritisautoimmune diseaseschildren

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The authors declare that there are no conflicts of interest present.