mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2014-3-247-256mimmun-690Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИССЛЕДОВАНИЕ ПРОЛИФЕРАТИВНОЙ АКТИВНОСТИ МОНОНУКЛЕАРНЫХ КЛЕТОК КРОВИ БОЛЬНЫХ РАССЕЯННЫМ СКЛЕРОЗОМ ПРИ ВОЗДЕЙСТВИИ ПЕПТИДА РЕГИОНА ENVELOPE ЭНДОГЕННОГО РЕТРОВИРУСА HERV-E λ4-1IN VITRO INDUCTION OF BLOOD MONONUCLEAR CELL PROLIFERATION BY ENDOGENOUS RETROVIRAL HERV-Eλ4-1 ENVELOPE PEPTIDE IN PATIENTS WITH MULTIPLE SCLEROSISСмагинА. А.SmaginA. A.

д.м.н., профессор, руководитель лаборатории лимфодетоксикации, клиника ФГБУ «Научно-исследовательский институт клинической и экспериментальной лимфологии» СО РАМН

ГольдинаИ. А.GoldinaI. A.

научный сотрудник лаборатории регуляции иммунопоэза ФГБУ «Научно-исследовательскийинститут клинической иммунологии» СО РАМН

igoldina@mail.ruГайдульК. В.GaidulK. V.

д.м.н., профессор, руководитель лаборатории регуляции иммунопоэза ФГБУ «Научно-исследовательский институт клинической иммунологии» СО РАМН

ЛюбарскийМ. С.LubarskyM. S.

д.м.н., профессор, член-корр. РАМН, заместитель директора по научно-клинической работе,клиника ФГБУ «Научно-исследовательский институт клинической и экспериментальной лимфологии» СО РАМН

Клиника ФГБУ «Научно-исследовательский институт клинической и экспериментальной лимфологии» СО РАМН, г. НовосибирскРоссияClinical Department, Research Institute of Clinical and Experimental Lymphology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskRussian FederationФГБУ «Научно-исследовательский институт клинической иммунологии» СО РАМН, г. НовосибирскРоссияResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskRussian Federation201401082014163247256Copyright © Смагин А.А., Гольдина И.А., Гайдуль К.В., Любарский М.С., 20142014Смагин А.А., Гольдина И.А., Гайдуль К.В., Любарский М.С.Smagin A.A., Goldina I.A., Gaidul K.V., Lubarsky M.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/690

С целью выявления иммуномодулирующих свойств синтетического олигопептида, гомологичного аминокислотной последовательности высококонсервативного региона протеина envelope эндогенного ретровируса человека HERV-Eλ4-1 было проведено сравнительное исследование пролиферативной активности мононуклеарных клеток крови здоровых лиц и больных рассеянным склерозом при воздействии данного олигопептида в культуре. Установлено, что исследуемый олигопептид стимулирует спонтанную и индуцированную митогенами пролиферацию мононуклеарных клеток крови как у доноров, так и у больных рассеянным склерозом. Выраженность стимулирующего воздействия олигопептида зависит от его концентрации в культуре и исходного уровня спонтанной пролиферации иммунокомпетентных клеток крови. Следовательно, олигопептид региона envelope эндогенного ретровируса HERV-Eλ4-1 повышает функциональную активность иммунокомпетентных клеток крови, что свидетельствует о наличии у него иммуностимулирующих свойств. Митогенная активность олигопептида региона envelope эндогенного ретровируса человека HERV-Eλ4-1 в отношении иммунокомпетентных клеток крови больных рассеянным склерозом, возможно, является одним из механизмов участия данного ретровируса в патогенезе заболевания.

A comparative in vitro study of blood mononuclear cells from multiple sclerosis patients and healthy donors was performed, in order to evaluate proliferative response to a retroviral antigen, aiming to determine immunomodulatory properties of synthetic oligopeptide homologous to a highly conserved human endogenous retrovirus HERV-Eλ4-1 envelope protein. It was revealed that this oligopeptide is able to stimulate the in vitro spontaneous and mitogen-induced proliferation of blood mononuclear cells from either donor and multiple sclerosis patients. Intensity of this oligopeptide-induced stimulatory effect depends on the protein concentration, and on initial level of blood immunocompetent cells proliferation. Hence, the endogenous retrovirus HERV-Eλ4-1 envelope region protein is able to increase functional activity of immunocompetent cells from human blood, that suggesting its immunostimulatory properties. It is possible that the mitogenic effects of this protein upon immunocompetent cells of multiple sclerosis patients represent a potential mechanism of retroviral involvement into pathogenesis of the disorder.

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The authors declare that there are no conflicts of interest present.