mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2014-3-237-246mimmun-689Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСЕКРЕЦИЯ ИНТЕРЛЕЙКИНОВ IL-12, IL-27 МОНОНУКЛЕАРНЫМИ ЛЕЙКОЦИТАМИ И ЭКСПРЕССИЯ ИХ РЕЦЕПТОРОВ НА Т-ЛИМФОЦИТАХ В УСЛОВИЯХ НАПРАВЛЕННОЙ ИНДУКЦИИ КЛЕТОК IN VITRO ПРИ ТУБЕРКУЛЕЗЕ ЛЕГКИХSECRETION OF IL-12, IL-27 BY MONONUCLEAR LEUKOCYTES AND EXPRESSION OF THEIR RECEPTORS ON T-LYMPHOCYTES IN CONDITIONS OF DIRECTED CELL INDUCTION IN VITRO IN PULMONARY TUBERCULOSISЕсимоваИ. Е.EsimovaI. E.

к.м.н., докторант кафедры патофизиологии ГБОУ ВПО «Сибирский государственный медицинский университет»

PhD (Medicine), Doctoral Candidate, Department of Pathophysiology, Siberian State Medical University

orevi@mail.ruУразоваО. И.UrazovaO. I.

д.м.н., профессор, профессор кафедры патофизиологии ГБОУ ВПО «Сибирский государственный медицинский университет»

PhD, MD (Medicine), Professor,Department of pathophysiology, Siberian State Medical University

НовицкийВ. В.NovitskiyV. V.

д.м.н., профессор, академик РАН, заведующий кафедрой патофизиологии ГБОУ ВПО ГБОУ ВПО «Сибирский государственный медицинский университет»

PhD, MD (Medicine), Full Member, Russian Academy of Sciences

ХасановаР. Р.KhasanovaR. R.

к.м.н., докторант кафедры патофизиологии ГБОУ ВПО «Сибирский государственный медицинский университет»

PhD (Medicine), Doctoral Candidate, Department of Pathophysiology, Siberian State Medical University

ФилинюкО. В.FilinyukO. V.

д.м.н., доцент, заведующая кафедрой фтизиатрии и пульмонологии ГБОУ ВПО «Сибирский государственный медицинский университет»

PhD, MD (Medicine), Associate Professor, Chief, Department of Phthisiology and Pulmonology, Siberian State Medical University

ГБОУ ВПО «Сибирский государственный медицинский университет» Минздрава России, г. ТомскРоссияSiberian State Medical University, TomskRussian Federation201401082014163237246Copyright © Есимова И.Е., Уразова О.И., Новицкий В.В., Хасанова Р.Р., Филинюк О.В., 20142014Есимова И.Е., Уразова О.И., Новицкий В.В., Хасанова Р.Р., Филинюк О.В.Esimova I.E., Urazova O.I., Novitskiy V.V., Khasanova R.R., Filinyuk O.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/689

В статье представлены результаты исследования у больных туберкулезом легких секреции цитокинов семейства интерлейкина (IL) 12 – IL-12р70, IL-12р40, IL-12р35 и IL-27 мононуклеарными лейкоцитами крови и экспрессии комплементарных им рецепторов на Т-лимфоцитах в условиях направленной (антиген- и цитокин-опосредованной соответственно) индукции клеток in vitro. У больных туберкулезом легких установлено угнетение спонтанной и BCG-индуцированной секреции IL- 12р70 и IL-12р35 на фоне гиперпродукции IL-12р40 и IL-27, снижение относительного количества СD3+gp130+, CD3+IL12Rβ2+ лимфоцитов и увеличение содержания Т-клеток с высокой экспрессией ингибиторной молекулы WSX-1 (CD3+WSX-1+hi) при IL-12/IL-27-индукции in vitro. Показано, что выявленные изменения в большинстве случаев носят однонаправленный характер и варьируют в зависимости от клинической формы заболевания и чувствительности возбудителя инфекции к противотуберкулезным препаратам.

The article presents the results of studies concerning secretion of cytokines belonging to interleukin 12 family, i.e., IL-12р70, IL-12р40, IL-12р35 and IL-27 in mononuclear blood leukocytes, as well as expression of their specific receptors on T-lymphocytes from tuberculosis patients under the in vitro conditions of directed (resp., antigen- and cytokine-mediated) cell induction. In patients with pulmonary TB, we have registered suppression of both spontaneous and BCG-induced secretion of IL-12р70 and IL- 12р35, along with overproduction of IL-12р40 and IL-27. Furthermore, a decrease in relative contents of СD3+gp130+,CD3+IL12Rβ2+ lymphocytes were revealed in the in vitro IL-12/IL-27 induction tests, as well as increased concentration of T-cells with high expression of the inhibitory molecule WSX-1 (CD3+WSX-1+hi). It was shown that the detectable changes are unidirectional in most cases, being dependent on clinical form of the disease and sensitivity of the etiological agent to anti-tuberculosis drugs.

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The authors declare that there are no conflicts of interest present.