mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2013-6-535-542mimmun-672Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESРОЛЬ ГЕНЕТИЧЕСКИХ ПОЛИМОРФИЗМОВ ГЕНА IL-1β (-31 Т/С) И IL-6 (-174 G/C) В ТЕЧЕНИИ ХРОНИЧЕСКИХ ВИРУСНЫХ ГЕПАТИТОВ В И С У ДЕТЕЙTHE ROLE OF GENETIC POLYMORPHISMS OF GENE IL-1β (-31 Т/С) AND IL-6 (-174 G/C) IN THE COURSE OF CHRONIC HEPATITIS B AND C IN CHILDRENРомановаС. В.RomanovaS. V.

к.м.н., ведущий научный сотрудник отдела «Клиника патологии верхних отделов пищеварительного тракта»

603950, Россия, г. Нижний Новгород, ул. Семашко, 22. Тел.: 8 (831) 436-15-90. Факс: 8 (831) 436-56-59

PhD (Medicine), Leading Research Associate, Department of Upper Gastrointestinal Tract Pathology

603950, Semashko str., 22. Phone: 7 (831) 436-15-90. Fax: 7 (831) 436-56-59

krigina@mail.ruВидмановаТ. А.VidmanovaТ. А.

к.м.н., заведующая отделом «Клиника патологии верхних отделов пищеварительного тракта»

PhD (Medicine), Chief, Department of Upper Gastrointestinal Tract Pathology

krigina@mail.ruЖуковаЕ. А.ZhukovaЕ. А.

д.м.н., профессор, заместитель директора по научной работе

PhD, MD (Medicine), Deputy Director for Research

krigina@mail.ruЕрмолинаЕ. В.ErmolinaЕ. V.

врач-лаборант клинико-диагностической лаборатории

Laboratory Doctor, Clinicodiagnostic Laboratory

krigina@mail.ruТолкачеваН. И.TolkachevaN. I.

к.б.н., старший научный сотрудник группы клинической иммунологии лабораторно-диагностического отдела

PhD (Biology), Senior Research Associate, Clinical Immunology Group, Laboratory & Diagnostic Division

krigina@mail.ruМаянскаяИ. В.MayanskayaI. V.

к.б.н., ведущий научный сотрудник группы клинической иммунологии лабораторно-диагностического отдела

PhD (Biology), Leading Research Worker, Clinical Immunology Group, Laboratory & Diagnostic division

krigina@mail.ruТимченкоИ. А.TimchenkoI. А.

младший научный сотрудник отдела «Клиника патологии верхних отделов пищеварительного тракта»

Junior Research Associate, Clinic of Upper Gastrointestinal Tract Pathology

krigina@mail.ruФГБУ «Нижегородский научно-исследовательский институт детской гастроэнтерологии» Минздрава России, г. Нижний НовгородРоссияNizhny Novgorod Research Institute of Children Gastroenterology, Ministry of Health of the Russian Federation, Nizhny NovgorodRussian Federation201326072014156535542Copyright © Романова С.В., Видманова Т.А., Жукова Е.А., Ермолина Е.В., Толкачева Н.И., Маянская И.В., Тимченко И.А., 20142014Романова С.В., Видманова Т.А., Жукова Е.А., Ермолина Е.В., Толкачева Н.И., Маянская И.В., Тимченко И.А.Romanova S.V., Vidmanova Т.А., Zhukova Е.А., Ermolina Е.V., Tolkacheva N.I., Mayanskaya I.V., Timchenko I.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/672

Резюме. Цель: выявить взаимосвязи полиморфизмов генов цитокинов IL-1β и IL-6 с особенностями клинического течения хронических вирусных гепатитов В и С у детей.

В исследование включено 48 детей в возрасте от 6 до 17 лет (медиана – 12 лет), из них 30 – с ХГС, 18 – с ХГВ. Выявление полиморфизма генов цитокинов – интерлейкина 1β (IL-1β) – T31C, интерлейкина 6 (IL-6) – G174C проводили методом аллельспецифичной полимеразной цепной реакции, детекция продуктов амплификации проводилась с помощью горизонтального электрофореза («Литех», Россия).

Больные ХГВ и ХГС характеризовались повышенной частотой генотипов СС гена IL-1β -31 Т/С и СС гена IL-6 -174 G/C по сравнению с контролем OR = 3,66 (95% CI – 1,2-11,0) и OR = 2,81 (95% CI – 1,33-5,87). Полиморфизм IL-6 -174 G/C ассоциирован с более высоким синтезом IL-1β у носителей генотипа GG по сравнению с GC (р = 0,016) и СС (р = 0,030). У детей с активным ХГВ и ХГС полиморфизмы IL-1β -31 ТС и СС, а также IL-6 -174 СC были связаны с более высоким уровнем цитолиза, а генотипы IL-1β -31 ТТ и IL-6 -174 GC и GG – с его отсутствием (р = 0,022; р = 0,038).

Наличие полиморфизмов генов IL-1β и IL-6, вероятно, вносит вклад в предрасположенность к развитию хронического гепатита и влияет на характер клинико-иммунологических проявлений болезни.

Abstract. Objectives: Chronic viral hepatitis В (CHB) and С (CHC) comprise an important issue of modern pediatrics. Immunogenetic disorders are among key factors of their pathogenesis. The aim of present research was to reveal interactions between polymorphisms of cytokine IL-1β and IL-6 genes and clinical characteristics of the disease in children.

The study included forty-eight children aged from 6 to 17 years (a median of 12 years old), including thirty patients with CHC, and eighteen – with CHB. Polymorphism of cytokine genes – interleukin 1β (IL-1β) – T31C, interleukin 6 (IL-6) – G174C was evaluated by means of allele-specific polymerase chain reaction (PCR); the amplification products were detected by horizontal electrophoresis (“Litekh”, Russia).

The groups of CHB and CHC patients were characterized by increased prevalence of CC genotype of IL- 1β Т/С -31gene and СС genotype of IL-6 -174 G/C gene variants, as compared to control group [OR = 3.66 (95% CI – 1.2-11.0) and OR = 2.81 (95% CI – 1.33-5.87)]. The IL-6 -174 G/C polymorphism of is associated with higher IL-1β synthesis in GG genotype carriers, when compared to GC (p = 0.016) and СС genotypes (p = 0.030). In children with active CHB and CHC, polymorphisms of IL-1β -31 ТС and СС, as well as IL-6 -174 СC, were related to the higher cytolysis level, while the genotypes IL-1β -31 ТТ and IL-6 -174 GC and GG – with absence of cytolytic features(p = 0.022; p = 0.038).

Presence of polymorphisms of IL-1β and IL-6 genes may sufficiently contribute to susceptibility to chronic hepatitis development and have an impact on extent of clinical and immunological presentations of the disease.

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The authors declare that there are no conflicts of interest present.