mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2013-5-449-456mimmun-661Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВЛИЯНИЕ СЕКРЕТОРНЫХ ПРОДУКТОВ ТКАНИ ПЛАЦЕНТЫ НА ЭКСПРЕССИЮ ПОВЕРХНОСТНЫХ РЕЦЕПТОРОВ КЛЕТКАМИ ЛИНИИ ТНР-1INFLUENCE OF SOLUBLE PLACENTADERIVED FACTORS UPON EXPRESSION OF SURFACE RECEPTORS ON THP-1 CELLSЛьвоваТ. Ю.LvovaT. Yu.

лаборант-исследователь лаборатории иммунологии

Research assistant, Department of Immunology

Falcojugger@yandex.ruОнохинаЯ. С.OnochinaYa. S.

лаборант-исследователь лаборатории иммунологии

Research assistant, Department of Immunology

Falcojugger@yandex.ruКореньковД. А.KorenkovD. A.

старший научный сотрудник лаборатории иммунологии

Senior Research Associate, Department of Immunology

Falcojugger@yandex.ruСтепановаО. И.StepanovaO. I.

научный сотрудник лаборатории иммунологии

Research Associate, Department of Immunology

Falcojugger@yandex.ruФураеваК. Н.FuraevaK. N.

студентка

Student, Department of Immunology

Falcojugger@yandex.ruПотолицынаЕ. А.PotolicinaE. A.

студентка

Student, Department of Immunology

Falcojugger@yandex.ruСельковС. А.SelkovS. A.

д.м.н., профессор, руководитель лаборатории иммунологии

PhD, MD (Medicine), Professor, Chief, Department of Immunology

Falcojugger@yandex.ruСоколовД. И.SokolovD. I.

д.б.н., старший научный сотрудник лаборатории иммунологии

199034, Менделеевская линия, 3. Тел.: 8 (812) 328-98-50

PhD, MD, Senior Research Associate, Department of Immunology

199034, Mendeleev Line, 3. Phone: 7 (812) 328-98-50

Falcojugger@yandex.ruФГБУ «Научно-исследовательский институт акушерства и гинекологии им. Д.О. Отта» СЗО РАМН, Санкт-ПетербургРоссияD. Ott Research Institute of Obstetrics and Gynecology, Russian Academy of Medical Sciences, St. PetersburgRussian Federation201325072014155449456Copyright © Львова Т.Ю., Онохина Я.С., Кореньков Д.А., Степанова О.И., Фураева К.Н., Потолицына Е.А., Сельков С.А., Соколов Д.И., 20142014Львова Т.Ю., Онохина Я.С., Кореньков Д.А., Степанова О.И., Фураева К.Н., Потолицына Е.А., Сельков С.А., Соколов Д.И.Lvova T.Y., Onochina Y.S., Korenkov D.A., Stepanova O.I., Furaeva K.N., Potolicina E.A., Selkov S.A., Sokolov D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/661

Резюме. В зоне маточно-плацентарного кровотока моноциты периферической крови матери подвергаются действию различных цитокинов и ростовых факторов, секретируемых тканью плаценты. Впоследствии эти клетки мигрируют в децидуальную ткань и играют важную роль в развитии и функционировании плаценты. Целью настоящего исследования явилось изучение влияния секреторных продуктов ткани плаценты, полученных при физиологической и осложненной гестозом беременностью, на экспрессию поверхностных рецепторов моноцитоподобными клетками линии ТНР-1. Секреторные продукты ткани плацент третьего триместра физиологической беременности приводили к снижению интенсивности экспрессии клетками линии ТНР-1 адгезионных молекул CD11а и CD18, а также молекул TRAIL, CD54, CD14 и VEGFR1 по сравнению с первым триместром. Под влиянием факторов, секретируемых тканью плаценты с гестозом, происходило увеличение интенсивности экспрессии молекул CD18 и CD54 и снижение интенсивности экспрессии VEGFR1 клетками линии ТНР-1 по сравнению с физиологической беременностью. Работа поддержана грантами Президента РФ №НШ-131.2012.7, СП-3492.2013.4, МК-1580.2013.7 и гранта РФФИ №13-04-00304 А.

Abstract. During the passage through the utero-placental circulation, peripheral blood monocytes are exposed to action of various soluble placenta-derived factors. Subsequently these cells migrate to placental tissue and play a key role in regulation of placental growth and development. We investigated the influence of placental secretory factors upon expression of THP-1 cells surface receptors during normal pregnancy, and pregnancy complicated with preeclampsia. Soluble placenta-derived factors produced by the third-trimester placenta caused reduced intensity of CD11а, CD18, CD54, CD14, TRAIL and VEGFR1 expression on THP-1 cells, as compared with the first-trimester placental extracts. Soluble placenta-derived factors from preeclamptic placenta caused an increased intensity of CD18 and CD54 expression by THP-1 cells and decreased intensity of VEGFR1 expression in comparison to normal pregnancy. The work was supported by grants of the President of the Russian Federation № НШ-131.2012.7, СП-3492.2013.4 МК-1580.2013.7 and by grant РФФИ № 13-04-00304 А.

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The authors declare that there are no conflicts of interest present.