mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2013-4-325-334mimmun-643Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESКЛЕТОЧНЫЕ ВЗАИМОДЕЙСТВИЯ ПРИ ОТВЕТЕ НА Т-НЕЗАВИСИМЫЕ АНТИГЕНЫ 2-ГО ТИПА IN VITROIN VITRO CELLULAR INTERACTIONS DURING IMMUNE RESPONSE TO T CELLINDEPENDENT TYPE 2 ANTIGENSГавриловаМ. В.GavrilovaM. V.

научный сотрудник лаборатории биосинтеза иммуноглобулинов

115533, Нагатинская наб., 18, кв. 90. Тел.: 8 (495) 674-08-42

Research Associate, Laboratory of Immunoglobulin Biosynthesis

115533, Nagatinskaya emb., 18, apt 90. Phone: 7 (495) 674-08-42

gavrilovamv@gmail.comЧернышоваИ. Н.ChernyshovaI. N.

к.м.н., старший научный сотрудник лаборатории биосинтеза иммуноглобулинов

PhD (Medicine), Senior Research Associate, Laboratory of Immunoglobulin Biosynthesis

gavrilovamv@gmail.comХоченковД. А.KhochenkovD. A.

к.б.н., научный сотрудник лаборатории биосинтеза иммуноглобулинов

PhD (Biology), Research Associate, Laboratory of Immunoglobulin Biosynthesis

gavrilovamv@gmail.comСидороваЕ. В.SidorovaE. V.

д.б.н., заведующая лабораторией биосинтеза иммуноглобулинов

PhD, MD (Biology), Chief, Laboratory of Immunoglobulin Biosynthesis

gavrilovamv@gmail.comФГБУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова» РАМН, МоскваРоссияI.I. Mechnikov Research Institute of Vaccines and Sera, MoscowRussian Federation201324072014154325334Copyright © Гаврилова М.В., Чернышова И.Н., Хоченков Д.А., Сидорова Е.В., 20142014Гаврилова М.В., Чернышова И.Н., Хоченков Д.А., Сидорова Е.В.Gavrilova M.V., Chernyshova I.N., Khochenkov D.A., Sidorova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/643

Abstract. Interactions between B1, B2 and bone marrow dendritic cells (DCs) of CBA mice were investigated in the course of immune response to T-independent antigen type 2 (TI 2 Ag) in model system. Splenocytes of Xid-mice CBA/N were used as “fillers”. It was shown that DCs augmented viability of B1 and B2 lymphocytes. Functional activity of the B1 and B2 lymphocytes was determined by ELISPOT method, as relative numbers of antibody- and immunoglobulin-forming cells (AFCs and IFCs, respectively) detectable after four days in culture. An increased immune response to TI 2 Ag in presence of DCs was observed mainly for B2 subpopulation, containing MZ-B lymphocytes as well. Specific effect of DC upon B2 cell subpopulation was confirmed by detection of direct in vitro interactions of DCs with T and B lymphocytes. DCs cultivated with B2 and T lymphocytes, used as fillers, decreased the numbers of AFCs and IFCs, irrespectively of absence or presence of TI-2 Ags. A decrease in AFC and IFC numbers in the cultures with B1 cells was evident in presence of the Ags only. It was shown that the inhibitory effect of DCs depended on their contact interactions. Separate cultures of B1 and B2 cells with DC under transwell conditions resulted into occurence of AFC and IFC in amounts similar to those generated in vitro without DCs.

В1-лимфоцитыТН-2-антигеныВ2-лимфоцитыдендритные клеткиB1 cellsTH lymphocytesТН2 antigensВ2 lymphocytesdendritic cellsReferencesГаврилова М.В., Чернышова И.Н., Сидорова Е.В. Роль различных субпопуляций В-клеток в иммунном ответе на Т-независимые антигены 2-го типа // Медицинская иммунология. – 2007. – Т. 9, № 1. – С. 39-46. Gavrilova M.V., Chernyshova I.N., Sidorova E.V. Rol` razlichnykh subpopulyatsiy B-kletok v immunnom otvete na T-nezavisimye antigeny 2-go tipa [Role of different B cell subpopulations in immune response to t-independent type 2 antigens]. Meditsinskaya immunologiya – Medical Immunology, 2007, vol. 1, no. 9, pp. 39-46.Гаврилова М.В., Чернышова И.Н., Сидорова Е.В. Роль различных субпопуляций В-клеток в иммунном ответе на Т-независимые антигены 2-го типа // Медицинская иммунология. – 2007. – Т. 9, № 1. – С. 39-46. Gavrilova M.V., Chernyshova I.N., Sidorova E.V. Rol` razlichnykh subpopulyatsiy B-kletok v immunnom otvete na T-nezavisimye antigeny 2-go tipa [Role of different B cell subpopulations in immune response to t-independent type 2 antigens]. Meditsinskaya immunologiya – Medical Immunology, 2007, vol. 1, no. 9, pp. 39-46.Сидорова Е.В. Субпопуляции В-лимфоцитов и их функциональная роль // Успехи современной биологии. – 2002. – Т. 122, № 5. – С. 467-479. Sidorova E.V. Subpopulyatsii B-limfotsitov i ikh funktsional`naya rol` [B-lymphocytes subpopulation and their functional role. Biology bulletin reviews]. Uspekhi sovremennoy biologii – Biology Bulletin Reviews, 2002, vol. 122, no. 5, pp. 467-479.Сидорова Е.В. Субпопуляции В-лимфоцитов и их функциональная роль // Успехи современной биологии. – 2002. – Т. 122, № 5. – С. 467-479. Sidorova E.V. Subpopulyatsii B-limfotsitov i ikh funktsional`naya rol` [B-lymphocytes subpopulation and their functional role. Biology bulletin reviews]. Uspekhi sovremennoy biologii – Biology Bulletin Reviews, 2002, vol. 122, no. 5, pp. 467-479.Хоченков Д.А. Роль дендритных клеток в иммунном ответе на Т-независимые антигены типа 2 // Биологические мембраны. – 2010. – Т. 27, № 4. – С. 1-6. Hochenkov D.A. Rol` dendritnykh kletok v immunnom otvete na T-nezavisimye antigeny tipa 2 [Role of dendritic cells in immune response to T-independent type 2 antigens]. Biologicheskie membrany – Membrane and Cell Biology. Biochemistry (Moscow) suppl. Series A., 2010, vol. 27, no. 4, pp. 1-6.Хоченков Д.А. Роль дендритных клеток в иммунном ответе на Т-независимые антигены типа 2 // Биологические мембраны. – 2010. – Т. 27, № 4. – С. 1-6. Hochenkov D.A. Rol` dendritnykh kletok v immunnom otvete na T-nezavisimye antigeny tipa 2 [Role of dendritic cells in immune response to T-independent type 2 antigens]. Biologicheskie membrany – Membrane and Cell Biology. Biochemistry (Moscow) suppl. Series A., 2010, vol. 27, no. 4, pp. 1-6.Чернышова И.Н., Гаврилова М.В., Сидорова Е.В. Модельная система для изучения клеточных взаимодействий и механизмов иммунного ответа на Т-независимые антигены 2-го типа in vitro // Биологические мембраны. – 2010. – Т. 27, № 5. – С. 1-7. Chernyshova I.N., Gavrilova M.V., Sidorova E.V. Model`naya sistema dlya izucheniya kletochnykh vzaimodeystviy i mekhanizmov immunnogo otveta na T-nezavisimye antigeny 2-go tipa in vitro [Model system to study cell interactions and mechanisms of immune response to to T-independent type 2 antigens in vitro]. Biologicheskie membrany – Membrane and Cell Biology. Biochemistry (Moscow) suppl. Series A., 2010, vol. 27, no. 5, pp. 1-7.Чернышова И.Н., Гаврилова М.В., Сидорова Е.В. Модельная система для изучения клеточных взаимодействий и механизмов иммунного ответа на Т-независимые антигены 2-го типа in vitro // Биологические мембраны. – 2010. – Т. 27, № 5. – С. 1-7. Chernyshova I.N., Gavrilova M.V., Sidorova E.V. Model`naya sistema dlya izucheniya kletochnykh vzaimodeystviy i mekhanizmov immunnogo otveta na T-nezavisimye antigeny 2-go tipa in vitro [Model system to study cell interactions and mechanisms of immune response to to T-independent type 2 antigens in vitro]. Biologicheskie membrany – Membrane and Cell Biology. Biochemistry (Moscow) suppl. Series A., 2010, vol. 27, no. 5, pp. 1-7.Breukels M., Rijkers G., Voorhorst-Ogink M., Zegers B. Regulatory T cells in the antibody response to Haemophilus nfluenza type b polysaccharide. Infection and Immunity, 1999, vol. 67, pp. 789-793.Breukels M., Rijkers G., Voorhorst-Ogink M., Zegers B. Regulatory T cells in the antibody response to Haemophilus nfluenza type b polysaccharide. Infection and Immunity, 1999, vol. 67, pp. 789-793.Lutz M.B., Kukutsch N.,Ogilvie A.L., R ssner S., Koch F., Romani N., Schuler G. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods, 1999, vol. 223, pp. 77-92.Lutz M.B., Kukutsch N.,Ogilvie A.L., R ssner S., Koch F., Romani N., Schuler G. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J. Immunol. Methods, 1999, vol. 223, pp. 77-92.Cobb B., Wang Q., Tzianabos O., Kasper D. Polysaccharide processing and presentation by the MHCII Pathway. Cell, 2004, vol. 117, no. 5, pp. 677-687.Cobb B., Wang Q., Tzianabos O., Kasper D. Polysaccharide processing and presentation by the MHCII Pathway. Cell, 2004, vol. 117, no. 5, pp. 677-687.Quah B., Warren H., Parish C. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nature Protocols, 2007, vol. 2, pp. 2049-2056.Quah B., Warren H., Parish C. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nature Protocols, 2007, vol. 2, pp. 2049-2056.Rademaekers A., Kolsch E., Specht C. T cell mediated antibody invariance in an immune response against a bacterial carbohydrate antigen requires CD28/B7-1 costimulation. Developmental Immunology, 2001, vol. 8, no. 3-4, pp. 243-257.Rademaekers A., Kolsch E., Specht C. T cell mediated antibody invariance in an immune response against a bacterial carbohydrate antigen requires CD28/B7-1 costimulation. Developmental Immunology, 2001, vol. 8, no. 3-4, pp. 243-257.Santos L., Draves K., Boton M., Grewal P., Marth J., Clark E. Dendritic cell-dependent inhibition of B cell proliferation requires CD22. J. Immunol., 2008, vol. 180, no. 7, pp. 4561-4569.Santos L., Draves K., Boton M., Grewal P., Marth J., Clark E. Dendritic cell-dependent inhibition of B cell proliferation requires CD22. J. Immunol., 2008, vol. 180, no. 7, pp. 4561-4569.Vos Q., Lees A., Wu Z., Snapper C., Mond J. B-cell activation by t-cell-independent type 2 antigens as an integral part of the humoral immune response to pathogenic microorganisms. Immunological Reviews, 2000, vol. 176, pp. 154-170.Vos Q., Lees A., Wu Z., Snapper C., Mond J. B-cell activation by t-cell-independent type 2 antigens as an integral part of the humoral immune response to pathogenic microorganisms. Immunological Reviews, 2000, vol. 176, pp. 154-170.Vos Q., Snapper C., Mond J. Th1 versus Th2 cytokine profile determines the modulation of in vitro T cell-independent type 2 responses by IL-4. International Immunology, 2000, vol. 12, pp. 1337-1345.Vos Q., Snapper C., Mond J. Th1 versus Th2 cytokine profile determines the modulation of in vitro T cell-independent type 2 responses by IL-4. International Immunology, 2000, vol. 12, pp. 1337-1345.Chelvarajan R.L., Raithatha R., Venkataraman C., Kaul R., Han S.S., Robertson D.A., Bondada S. CpG oligodeoxynucleotides overcome the unresponsiveness of neonatal B cells to stimulation with the thymusindependent stimuli anti-IgM and TNP-Ficoll. Eur. J. Immunol., 1999, vol. 29, pp. 2808-2818.Chelvarajan R.L., Raithatha R., Venkataraman C., Kaul R., Han S.S., Robertson D.A., Bondada S. CpG oligodeoxynucleotides overcome the unresponsiveness of neonatal B cells to stimulation with the thymusindependent stimuli anti-IgM and TNP-Ficoll. Eur. J. Immunol., 1999, vol. 29, pp. 2808-2818.

The authors declare that there are no conflicts of interest present.