mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2013-3-227-234mimmun-631Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВЛИЯНИЕ ФАКТОРОВ, СЕКРЕТИРУЕМЫХ ТКАНЬЮ ПЛАЦЕНТЫ, НА СЕКРЕЦИЮ ЦИТОКИНОВ МОНОЦИТОПОДОБНОЙ ЛИНИЕЙ КЛЕТОК ТНР-1EFFECTS OF SECRETABLE PLACENTAL FACTORS UPON SECRETION OF CYTOKINES BY THP-1 MONOCYTE-LIKE CELLSОнохинаЯ. С.OnokhinaYa. S.

лаборант-исследователь лаборатории иммунологии с группой по диагностике СПИД

Investigator (Biologist), Laboratory of Immunology with an AIDS Diagnostic Group

Falcojugger@yandex.ruЛьвоваТ. Ю.LvovaT. Yu.

лаборант-исследователь лаборатории иммунологии с группой по диагностике СПИД

Investigator (Biologist), Laboratory of Immunology with an AIDS Diagnostic Group

Falcojugger@yandex.ruЦицкараваД. З.TzitzkaravaD. Z.

ординатор лаборатории иммунологии с группой по диагностике СПИД

Resident Physician, Laboratory of Immunology with an AIDS Diagnostic Group

Falcojugger@yandex.ruСельковС. А.SelkovS. A.

д.м.н., профессор, заведующий лабораторией иммунологии с группой по диагностике СПИД

PhD, MD (Medicine), Professor, Chief, Laboratory of Immunology with an AIDS Diagnostic Group

Falcojugger@yandex.ruСоколовД. И.SokolovD. I.

д.б.н., ведущий научный сотрудник лаборатории иммунологии с группой по диагностике СПИД

199034, Менделеевская линия, 3. Тел./факс: 8 (812) 328-98-50, 323-75-45

PhD, MD (Biology), Leading Researcher, Laboratory of Immunology with an AIDS Diagnostic Group

Falcojugger@yandex.ruФГБУ «Научно-исследовательский институт акушерства и гинекoлогии им. Д.О. Отта» СЗО РАМН, Санкт-ПетербургРоссияD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgRussian Federation201324072014153227234Copyright © Онохина Я.С., Львова Т.Ю., Цицкарава Д.З., Сельков С.А., Соколов Д.И., 20142014Онохина Я.С., Львова Т.Ю., Цицкарава Д.З., Сельков С.А., Соколов Д.И.Onokhina Y.S., Lvova T.Y., Tzitzkarava D.Z., Selkov S.A., Sokolov D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/631

Резюме. Находясь в маточно-плацентарном кровотоке, моноциты подвергаются действию факторов, секретируемых тканью плаценты, которые влияют на их функциональное состояние при беременности. В настоящем исследовании на модели in vitro проведено сравнительное изучение влияния растворимых продуктов ткани плацент, полученных от женщин с физиологической и осложненной гестозом беременностью, на секрецию цитокинов моноцитоподобными клетками линии ТНР-1. Установлено, что секреция VEGF клетками линии ТНР-1 была выше, а секреция IL-6, IL-8 и MCP-1 была ниже в присутствии растворимых факторов ткани плацент третьего триместра беременности по сравнению с первым триместром беременности. Секреция IL-6 и MCP-1 клетками линии ТНР-1 была выше, а секреция растворимой формы рецептора TNFRII была ниже в присутствии растворимых факторов ткани плацент женщин с гестозом по сравнению с физиологической беременностью.

Работа выполнена при финансовой поддержке Министерства образования и науки РФ ГК № 02.740.11.0711 и грантов Президента РФ № НШ-3594.2010.7 и МД-150.2011.7.

Abstract. Мonocytes in feto-placental circulation are exposed to factors secreted by placental tissue. These factors influence monocyte functions in pregnancy. In present study, an in vitro model (monocyte-like THP-1 cells) was used for assessing effects of soluble placental factors obtained from women with physiological pregnancies, or preeclampsia cases. The following effects of placental factors were revealed: increased secretion of VEGF by THP-1 cells along with decreased secretion of IL-6, IL-8 and MCP-1 under the influence of placental factors from the I. trimester of pregnancy in comparison with III. trimester. Secretion of IL-6 and MCP-1 by THP-1 cells was increased, and secretion of soluble TNFRII was decreased upon co-cultivation with soluble placental factors from the women with preeclampsia, as compared with placental products from physiological pregnancies.

The work is supported by grants ГК № 02.740.11.0711 from Ministry of Education and Science, and НШ-3594.2010.7 grant from the President of Russian Federation.

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The authors declare that there are no conflicts of interest present.