mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2013-3-235-246mimmun-626Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСТИМУЛЯЦИЯ ДЕНДРИТНЫМИ КЛЕТКАМИ IN VITRO ПРОТИВООПУХОЛЕВОЙ ЦИТОТОКСИЧЕСКОЙ АКТИВНОСТИ МОНОНУКЛЕАРНЫХ КЛЕТОК БОЛЬНЫХ КОЛОРЕКТАЛЬНЫМ РАКОМIN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTSКурилинВ. В.KurilinV. V.

к.м.н., старший научный сотрудник лаборатории молекулярной иммунологии

PhD (Medicine), Senior Research Associate, Laboratory of Molecular Immunology

sennikov_sv@mail.ruХантаковаЮ. Н.KhantakovaYu. N.

аспирант

PhD Candidate

sennikov_sv@mail.ruОблеуховаИ. А.ObleukhovaI. A.

младший научный сотрудник лаборатории молекулярной иммунологии

Research Associate, Laboratory of Molecular Immunology

sennikov_sv@mail.ruШевченкоЮ. А.ShevchenkoYu. A.

к.б.н., научный сотрудник лаборатории молекулярной иммунологии

PhD (Biology), Research Associate, Laboratory of Molecular Immunology

sennikov_sv@mail.ruКуликоваЕ. В.KulikovaE. V.

аспирант

PhD Candidate

sennikov_sv@mail.ruЯкушенкоВ. К.YakushenkoV. K.

Городской центр колопроктологии

д.м.н., заведующий 2-м хирургическим отделением

City Center of Coloproctology

PhD, MD (Medicine), Chief, Second Surgical Department

sennikov_sv@mail.ruСоколовА. В.SokolovA. V.

заведующий 2-м онкологическим отделением

Chief, Second Department of Oncology

sennikov_sv@mail.ruСенниковС. В.SennikovS. V.

д.м.н., профессор, заведующий лабораторией молекулярной иммунологии

630099, Россия, г. Новосибирск, ул. Ядринцевская, 14. Тел.: 8 (383) 222-19-10. Факс: 8 (383) 222-70-28

PhD, MD (Medicine), Professor, Chief, Laboratory of Molecular Immunology

sennikov_sv@mail.ruФедеральное государственное бюджетное учреждение «Научно-исследовательский институт клинической иммунологии» Сибирского отделения РАМН, г. НовосибирскРоссияResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskRussian FederationМБУЗ ГКБ № 11, г. НовосибирскРоссияClinical Hospital No. 1, NovosibirskRussian FederationМБУЗ ГКБ № 1, г. НовосибирскРоссияCity Clinical Hospital No. 1, NovosibirskRussian Federation201324072014153235246Copyright © Курилин В.В., Хантакова Ю.Н., Облеухова И.А., Шевченко Ю.А., Куликова Е.В., Якушенко В.К., Соколов А.В., Сенников С.В., 20142014Курилин В.В., Хантакова Ю.Н., Облеухова И.А., Шевченко Ю.А., Куликова Е.В., Якушенко В.К., Соколов А.В., Сенников С.В.Kurilin V.V., Khantakova Y.N., Obleukhova I.A., Shevchenko Y.A., Kulikova E.V., Yakushenko V.K., Sokolov A.V., Sennikov S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/626

Abstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) derived from adherent fraction of peripheral blood mononuclear cells (PBMSc) obtained from patients with colorectal cancer. The DCs were then cocultured with non-adherent mononuclear cells, either in presence of IL-12 and IL-18, or without cytokines. The modulation efficiency was assessed as cytotoxic activity of mononuclear cells towards autologous tumor cells, production of IFNγ, IL-2, IL-4 and the number of perforin-, granzyme B-containing cells. We have demonstrated the ability of dendritic cells to stimulate the in vitro antitumor cytotoxic and secretory activity of effector cells in culture. A combination of dendritic cells with IL-12 and IL-18 is shown to enhance their effects in the mononuclear cells culture, as evidenced by increased percentage of dead autologous tumor cells, higher numbers of perforin(+), granzyme B(+) lymphocytes, and increased production of IFNγ, IL-2 increased.

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The authors declare that there are no conflicts of interest present.