mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2005-5-6-503-510mimmun-598Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESГЕТЕРОСУБТИПИЧЕСКИЙ ИММУННЫЙ ОТВЕТ И ЗАЩИТА ОТ ВЫСОКОПАТОГЕННОГО ВИРУСА ГРИППА А (H5N1) У МЫШЕЙ, ИММУНИЗИРОВАННЫХ ХОЛОДОАДАПТИРОВАННЫМ ВИРУСОМ ГРИППА А/ЛЕНИНГРАД/134/17/57(H2N2)HETEROSUBTYPIC IMMUNE RESPONSE AND CROSS-PROTECTION AGAINST A HIGHLY PATHOGENIC A (H5N1) INFLUENZA VIRUS IN MICE IMMUNIZED WITH COLD-ADAPTED A/LENINGRAD/134/17/57 (H2N2) INFLUENZA VIRUSРекстинА. Р.RekstinA. R.

197376, Санкт–Петербург, ул. Акад. Павлова, д. 12

vaccine@mail.ruДешеваЮ. А.DeshevaJ. A.vaccine@mail.ruЛуХ.LuX.vaccine@mail.ruАлександроваГ. И.AlexandrovaG. I.vaccine@mail.ruКлимовА. И.KlimovA. I.vaccine@mail.ruКацД. М.KatzJ. M.vaccine@mail.ruРуденкоЛ. Г.RudenkoL. G.vaccine@mail.ruНИИ экспериментальной медицины РАМН, Санкт–ПетербургRussian FederationЦентр по контролю за заболеваемостью, АтлантаUnited States20052307201475-6503510Copyright © Рекстин А.Р., Дешева Ю.А., Лу Х., Александрова Г.И., Климов А.И., Кац Д.М., Руденко Л.Г., 20142014Рекстин А.Р., Дешева Ю.А., Лу Х., Александрова Г.И., Климов А.И., Кац Д.М., Руденко Л.Г.Rekstin A.R., Desheva J.A., Lu X., Alexandrova G.I., Klimov A.I., Katz J.M., Rudenko L.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/598

While investigating the efficacy of an H5N2 ca reassortant vaccine candidate in protecting against a lethal challenge with a highly pathogenic (HP) H5N1 virus in the mouse model, we observed a degree of cross-protection provided by the ca Len/17 (H2N2) virus itself. 80% of mice administered a high dose of attenuated Len/17 vaccine intranasally (i.n.) survived after a lethal challenge with A/Hong Kong/483/97 H5N1 virus. Therefore, we investigated the basis of the cross- reactive immunity between H2N2 and H5N1 viruses that may have contributed to recovery from lethal HK/ 483 virus infection. Sera from mice immunized i.n. with Len/17 did not cross-react with HK/483 virus in neutralization or hemagglutination-inhibition assays, however IgG and IgA antibodies that cross-reacted with the hemagglutinin and neuraminidase of H5N1 1997 viruses were detected. Spleen cells from mice immunized i.n. with Len/17 vaccine showed enhanced production of IL-2, IL-4, IL-5, IL-10, and IFNγ following in vitro stimulation with inactivated H5N1 virus. Our findings indicate that both cross-reactive humoral and cellular immunity induced by Len/17 H2N2 vaccine may plays a role in recovery from lethal H5N1 virus infection. A better understanding of the mechanisms of heterosubtypic immunity will improve vaccine design against HP avian influenza viruses.

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The authors declare that there are no conflicts of interest present.