mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2012-4-5-295-304mimmun-461Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОЦЕНКА СОДЕРЖАНИЯ РАЗЛИЧНЫХ Т-ЭФФЕКТО РНЫХ СУБПОПУЛЯЦИЙ У ДЕТЕЙ И ВЗРОСЛЫХ МЕТО ДОМ ВНУТРИКЛЕТОЧНОГО ОКРАШИВАНИЯ ЦИТОКИНОВEVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINESЛагереваЮ. Г.LagerevaYu. G.

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anna-lagereva@yandex.ruМеньшиковС. В.MenshikovS. V.anna-lagereva@yandex.ruСавиноваТ. Л.SavinovaT. L.anna-lagereva@yandex.ruБейкинЯ. Б.BeykinJ. B.anna-lagereva@yandex.ruЧерешневВ. А.ChereshnevV. A.anna-lagereva@yandex.ruМБУ «Клинико-диагностический Центр», г. ЕкатеринбургRussian FederationУправление здравоохранения администрации, г. ЕкатеринбургRussian FederationИнститут иммунологии и физиологии УрО РАН, г. ЕкатеринбургRussian Federation201221072014144-5295304Copyright © Лагерева Ю.Г., Меньшиков С.В., Савинова Т.Л., Бейкин Я.Б., Черешнев В.А., 20142014Лагерева Ю.Г., Меньшиков С.В., Савинова Т.Л., Бейкин Я.Б., Черешнев В.А.Lagereva Y.G., Menshikov S.V., Savinova T.L., Beykin J.B., Chereshnev V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/461

Abstract. An intracellular staining technique of marker cytokines was applied for evaluation of quantitative distribution of T-lymphocyte effector subsets in children and adults. The results of this study have shown that the numbers of Th2 lymphocytes in infants (7 to 12 months old) correspond to appropriate normal values in adults, being accompanied by decreased Th1 and Тс1cell contents, whereas absolute amounts of Tc2 lymphocytes proved to be higher than in all other age groups. Absolute counts of Th17 and Tnc17 subsets in infants of 7 to 12 months were higher than in adults. Hence, these infants exhibited a shift of immune response towards Th2 (Tc2) and Th17 (Tnc17), thus presenting a potential factor of Th2-mediated allergic disorders in the children under one year. In children over 1 year, absolute amounts of Th1 and Тс1, as well as Th2 and Тс2 lymphocyte counts did not significantly differ from adult values. Th2 lymphocyte counts among adolescents (15 to 18 years) were decreased, along with relative increase of IL-17А-positive lymphocyte scores, thus reflecting a probable predisposition for autoimmune disorders induced at this age. The age-dependent increase in Th1 and Тс1 counts in children may immediately correlate with formation of T-cell memory pool.

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The authors declare that there are no conflicts of interest present.