mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2009-6-509-514mimmun-351Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСРАВНЕНИЕ ЭФФЕКТОВ РАЗНЫХ ХЕМОТИПОВ ЛИПОПОЛИСАХАРИДОВ ИЗ ESCHERICHIA COLI И SALMONELLA НА СИНТЕЗ TNFα И IL-6 МАКРОФАГОПОДОБНЫМИ КЛЕТКАМИ ТНР-1A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLSВолошинаЕ. В.VoloshinaE. V.

142290, ул. Институтская, 2. Тел.: (4967) 73-07-44. Факс: (4967) 33-05-32

ewgenia2006@rambler.ruЗубоваС. В.ZubovaS. V.ewgenia2006@rambler.ruПрохоренкоС. В.ProkhorenkoS. V.ewgenia2006@rambler.ruКосяковаН. И.KosjakovaN. I.

отделение аллергологии и иммунологии

ewgenia2006@rambler.ruПрохоренкоИ. Р.ProkhorenkoI. R.ewgenia2006@rambler.ruИнститут фундаментальных проблем биологии РАН, г. ПущиноRussian FederationМосковская медицинская академия им. И.М. Сеченова, МоскваRussian FederationПНЦ РАН, г. ПущиноRussian FederationИнститут фундаментальных проблем биологии РАН, г. Пущино; Московская медицинская академия им. И.М. Сеченова, МоскваRussian Federation200920072014116509514Copyright © Волошина Е.В., Зубова С.В., Прохоренко С.В., Косякова Н.И., Прохоренко И.Р., 20142014Волошина Е.В., Зубова С.В., Прохоренко С.В., Косякова Н.И., Прохоренко И.Р.Voloshina E.V., Zubova S.V., Prokhorenko S.V., Kosjakova N.I., Prokhorenko I.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/351

Резюме. Проведено исследование влияния ЛПС, различающихся по структуре липидного или полисахаридного фрагмента, на индукцию цитокинов TNFα и IL-6. Исследование проводилось на линии клеток THP-1, дифференцированных в макрофагоподобные клетки с помощью форболмиристат-ацетата (РМА). В работе использовали ЛПС S. typhimurium (S-хемотип), S. Typhimurium SL1181 (R-хемотип), E. coli O55:B5 (S-хемотип) и E. coli JM103 (R-хемотип). Показано, что уменьшение молярной доли липида А/КДО в S-форме ЛПС из E. coli сопровождается пониженной экспрессией TNFα и IL-6. Наоборот, для S-формы ЛПС из Salmonella снижение молярной доли липида А/КДО значительно усиливало экспрессию TNFα. Для R-хемотипов вклад структуры липида А в биологическую активность ЛПС более значим, чем для S, независимо от вида бактерий.

Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.

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The authors declare that there are no conflicts of interest present.