mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-CTA-3182mimmun-3182Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESCXCR1, TLR4 и CXCL8: ключевые факторы вирулентности Pseudomonas aeruginosa при раневых и ожоговых инфекцияхCXCR1, TLR4, and CXCL8: Key Mediators of Pseudomonas aeruginosa Virulence in Wound and Burn Infectionshttps://orcid.org/0000-0002-8276-0825МохсинМуслим ИданMohsinMuslim Idan

PhD, доцент кафедры патологических анализов, научный факультет

PhD, Assistant Professor, Department of Pathological Analyses, Faculty of Science

muslim.aljabri@uokufa.edu.iqХальфаХайдар МухсинKhalfaHydar Muhsin

PhD, кафедра биологии, научный факультет

PhD, Department of Biology, University of Kufa, Faculty of Science

hydarm.jmaiwai@uokufa.edu.iqАль-ХилалиСамер А.М.Х.Al-HilaliSamer A. M. H.

кафедра патологических анализов, научный факультет

Department of Pathological Analyses, Faculty of Science

sameram.hussein@uokufa.edu.iqАль-ЗубайдиИсраа Махмуд КадимAl-ZubaidyIsraa Mahmood Kadhim

магистр наук, кафедра патологических анализов, научный факультет

MSC, Department of Pathological Analyses, Faculty of Science

omeralgburi58@gmail.comДжаббарМахмуд ШакерJabbarMahmood Shaker

магистр наук

MSC

mahtech11@gmail.comЗайедКаррар С.ZayedKarrar S.

PhD, кафедра патологических анализов, научный факультет

PhD, Department of Pathological Analyses, Faculty of Science

karrars.alshebly@uokufa.edu.iqАль-ШамартиМохаммад ЯсимAl-ShamartiMohammed Jasim

PhD, кафедра патологических анализов, научный факультет

PhD, Department of Pathological Analyses, Faculty of Science

mohammed.alshemirti@uokufa.edu.iqУниверситет КуфыИракUniversity of KufaIraqМедицинская больница Аль-Садер, Управление здравоохранения Эн-НаджафаИракMedical Al-Sader Hospital, Al-Najaf Health OfficeIraq20252012202527613011310Copyright © Мохсин М.И., Хальфа Х.М., Аль-Хилали С.А., Аль-Зубайди И.М., Джаббар М.Ш., Зайед К.С., Аль-Шамарти М.Я., 20252025Мохсин М.И., Хальфа Х.М., Аль-Хилали С.А., Аль-Зубайди И.М., Джаббар М.Ш., Зайед К.С., Аль-Шамарти М.Я.Mohsin M.I., Khalfa H.M., Al-Hilali S.A., Al-Zubaidy I.M., Jabbar M.S., Zayed K.S., Al-Shamarti M.J.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/3182

Синегнойная палочка (Pseudomonas aeruginosa) является условно патогенным микроорганизмом, имеющим важное клиническое значение и представляет собой серьезную проблему для восприимчивых лиц, особенно в условиях нозокомиальных учреждений. Люди с ослабленной иммунной системой, с установленными катетерами и обширными ожоговыми травмами особенно уязвимы к ее коварным и часто разрушительным последствиям. Данное исследование было направлено на выяснение роли Toll-подобного рецептора 4 (TLR4), Toll-подобного рецептора 5 (TLR5), хемокина CXCL8 и его рецептора CXCR1 в плане инфекции P. aeruginosa. Наши результаты указывают на значительную роль TLR4 и CXCL8 в реакции организма на этот патоген. Примечательно, что экспрессия CXCR1 была снижена как в клеточных моделях, так и в образцах цельной крови, полученных от пациентов с бактериальными инфекциями, в частности вызванными P. aeruginosa. Используя антитела, направленные на эти молекулы клеточной поверхности, мы дополнительно исследовали их влияние на бактериальную адгезию и модуляцию инфекционного процесса. Применение антител к CXCR1 привело к заметному усилению бактериальной инфекции в линиях клеток T24 и A549. Напротив, введение антител к TLR4 оказывало ингибирующее действие на инфекцию P. aeruginosa. Однако антитела к CXCL8 не оказывали заметного влияния на ход бактериальной инфекции в течение первых трех часов после воздействия. Эти наблюдения предполагают двойную роль этих молекул в ответе организма на P. aeruginosa: CXCR1, по-видимому, функционирует как отрицательный регулятор, в то время как TLR4, по-видимому, действует как положительный регулятор в контексте бактериальной инфекции.

Pseudomonas aeruginosa, an opportunistic pathogen of considerable clinical import, presents a formidable challenge to susceptible individuals, particularly within the confines of nosocomial settings. Those with compromised immune systems, indwelling medical devices such as catheters, and extensive thermal injuries are especially vulnerable to its insidious and often devastating effects. This investigation sought to elucidate the roles of Toll-like receptor 4 (TLR4), Toll-like receptor 5 (TLR5), the chemokine CXCL8, and its cognate receptor CXCR1 in the context of P. aeruginosa infection. Our findings indicate a significant involvement of TLR4 and CXCL8 in the host response to this pathogen. Notably, CXCR1 expression was observed to be downregulated both in cellular models and in whole blood samples obtained from patients afflicted with bacterial infections, specifically those caused by P. aeruginosa. Utilizing antibodies targeting these cell surface molecules, we further explored their influence on bacterial adhesion and the modulation of infection. The application of anti-CXCR1 antibodies resulted in a demonstrable increase in bacterial infection in both T24 and A549 cell lines. Conversely, the administration of anti-TLR4 antibodies exerted an inhibitory effect on P. aeruginosa infection. Anti-CXCL8 antibodies, however, did not elicit a discernible impact on bacterial infection within the initial three hours of exposure. These observations suggest a dichotomous role for these molecules in the host response to P. aeruginosa: CXCR1 appears to function as a negative regulator, while TLR4 appears to act as a positive regulator in the context of bacterial infection.

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The authors declare that there are no conflicts of interest present.