mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2009-2-3-227-238mimmun-308Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОСНОВНЫЕ И МАЛЫЕ ПОПУЛЯЦИИ ЛИМФОЦИТОВ ПЕРЕФЕРИЧЕСКОЙ КРОВИ ЧЕЛОВЕКА И ИХ НОРМАТИВНЫЕ ЗНАЧЕНИЯ (МЕТОДОМ МНОГОЦВЕТНОГО ЦИТОМЕТРИЧЕСКОГО АНАЛИЗА)MAJOR AND LYMPHOCYTE POPULATIONS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES AND THEIR REFERENCE VALUES, AS ASSAYED BY MULTI-COLOUR CYTOMETRYХайдуковС. В.KhaidukovS. V.

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khsergey54@mail.ruЗурочкаА. В.ZurochkaA. V.khsergey54@mail.ruТотолянАрег А.TotolianAreg A.khsergey54@mail.ruЧерешневВ. А.ChereshnevV. A.khsergey54@mail.ruИнститут биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, МоскваRussian FederationГОУ ВПО Челябинская государственная медицинская академия РОСЗДРАВА, г. ЧелябинскRussian FederationФГУН НИИ эпидемиологии и микробиологии имени Пастера РоспотребнадзораРоссияSt. Petersburg Pasteur Institute of Epidemiology and MicrobiologyRussian FederationИнститут иммунологии и физиологии УрО РАН, г. ЕкатеринбургRussian Federation200919072014112-3227238Copyright © Хайдуков С.В., Зурочка А.В., Тотолян А.А., Черешнев В.А., 20142014Хайдуков С.В., Зурочка А.В., Тотолян А.А., Черешнев В.А.Khaidukov S.V., Zurochka A.V., Totolian A.A., Chereshnev V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/308

Резюме. В настоящее время определение субпопуляционного состава и фенотипа лимфоцитов является важным диагностическим признаком для выяснения нарушений связанных с функционированием иммунной системы. Однако информация, извлекаемая в результате анализа только основных популяций лимфоцитов периферической крови, является недостаточной. Для целей диагностики более важным представляется информация о наличии малых субпопуляций лимфоцитов и активированных пулов клеток.

Проведенные исследования субпопуляционного состава клеток периферической крови условно здоровых доноров в различных регионах России позволили определить интервалы распределения содержания как основных, так и малых субпопуляций клеток иммунной системы человека. Сравнение полученных результатов с литературными данными, опубликованными в зарубежной литературе, свидетельствуют о близости референтных интервалов распределения основных субпопуляций клеток иммунной системы условно здоровых доноров вне зависимости от места проживания.

Результатом данной работы стала разработка алгоритмов цитометрических исследований и формирование комбинаций моноклональных антител, позволяющих оценить как все основные популяции лимфоцитов, так и их минорные субпопуляции, участвующие в развитии иммунного ответа. Были определены интервалы распределения таких минорных субпопуляций, как В1- и В2-лимфоцитов, В-клеток памяти, γδ- и αβT-клеток, регуляторных и нативных Т-клеток, цитотоксической и секретирующей популяций NK-клеток.

Abstract. Determination of lymphocyte subpopulations and their phenotypes is an important diagnostic feature, in order to elucidate some disturbances connected with immune system functioning. However, insufficient data are obtained when analyzing only major populations of peripheral lymphocytes. In order to perform clinical diagnostics, the data about minor lymphocytic populations and activated cellular pools seem to be more pertinent.

Studies of peripheral blood cell subpopulations of healthy donors performed in different Russian regions allowed to assess quantitative distribution intervals for both major and minor immune cell subpopulations in humans. The results obtained, as compared with data from literature, provide an evidence for similar reference intervals for main immune cell subpopulations in healthy donors, independent on their habitation area.

Present work has resulted into development of algorithms for cytometric studies and generation of certain panels of monoclonal antibodies enabling evaluation of all main lymphocyte subpopulations, as well as their minor subsets participating in emerging immune response. The distribution intervals have been estimated for such minor subpopulations, as B1- and B2-lymphocytes, memory B-cells, γδ- and αβT-cells, regulatory and naїve T-cells, cytotoxic and secretory NK-cell polupations.

The results of present study, while been performed with peripheral blood of healthy donors, may provide a basis of reference values when studying subpopulation profile of immune cells.

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The authors declare that there are no conflicts of interest present.