mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-ETR-3021mimmun-3021Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОценка взаимоотношений между папилломавирусной инфекцией, раком предстательной железы и уровнями IL-12Evaluating the relationship between human papilloma virus infections, prostate cancer and interleukin-12 levelsМорадиС.MoradiS.

Садех Моради – отдел иммунологии, медицинский факультет 

P.O. Box 61355-45, Ахваз

Sadegh Moradi, Department of Immunology, Faculty of Medicine 

P.O. Box 61355-45, Ahvaz

РашноМ.RashnoM.

Мохаммад Рашно – ассистент Центра микробиологии, клеточных и молекулярных исследований 

P.O. Box 61355-45, Ахваз

Mohammad Rashno, Assistant Professor of Microbiology Cellular and Molecular Research Center 

P.O. Box 61355-45, Ahvaz

СаркарянМ.SarkarianM.

Мохсен Саркарян – ассистент кафедры урологии, Школа медицины, госпиталь Голестан 

P.O. Box 61355-45, Ахваз

Mohsen Sarkarian, Assistant Professor of Urology Department of Urology, School of Medicine, Golestan Hospital 

P.O. Box 61355-45, Ahvaz

КайданиГ. А.KaydaniG. A.

Голам Аббас Кайдани – ассистент кафедры медицинской вирусологии, отдел лабораторных наук, Общая школа медицины 

P.O. Box 61355-45, Ахваз

Gholam Abbas Kaydani, Assistant Professor of Medical Virology Department of Laboratory Sciences, School of Allied Medical Sciences 

P.O. Box 61355-45, Ahvaz

ФатахиЛ.FatahiL.

Ладан Фатахи – ассистент кафедры патологии, Школа медицины, госпиталь имама Хомейни, госпиталь Голестан 

P.O. Box 61355-45, Ахваз

Ladan Fatahi, Assistant Professor of Pathology Department of Pathology, School of Medicine,Imam Khomeini Hospital, Golestan Hospital 

P.O. Box 61355-45, Ahvaz

СейедтабибМ.SeyedtabibM.

Мариам Сейедтабиб – ассистент кафедры биостатистики, отдел биостатистики и эпидемиологии, Школа здравоохранения 

P.O. Box 61355-45, Ахваз

Maryam Seyedtabib, Assistant Professor of Biostatistics Department of Biostatistics and Epidemiology, School of Health 

P.O. Box 61355-45, Ahvaz

ШариффатМ.ShariffatM.

Муса Шариффат – отдел иммунологии, медицинский факультет 

P.O. Box 61355-45, Ахваз

Moosa Shariffat, Department of Immunology, Faculty of Medicine 

P.O. Box 61355-45, Ahvaz

ХодададиА.KhodadadiA.

Али Ходадади – отдел иммунологии, медицинский факультет 

P.O. Box 61355-45, Ахваз

Ali Khodadadi, Department of Immunology, Faculty of Medicine 

P.O. Box 61355-45, Ahvaz

akhodadadi2@gmail.comУниверситет медицинских наук Ахваза ДжундишапураИранAhvaz Jundishapur University of Medical SciencesIslamic Republic of Iran20251510202527510431052Copyright © Моради С., Рашно М., Саркарян М., Кайдани Г.А., Фатахи Л., Сейедтабиб М., Шариффат М., Ходадади А., 20252025Моради С., Рашно М., Саркарян М., Кайдани Г.А., Фатахи Л., Сейедтабиб М., Шариффат М., Ходадади А.Moradi S., Rashno M., Sarkarian M., Kaydani G.A., Fatahi L., Seyedtabib M., Shariffat M., Khodadadi A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/3021

Рак предстательной железы (РПЖ) является второй по значимости причиной смертности от рака среди мужчин. Вирус папилломы человека (ВПЧ) является наиболее распространенной причиной рака шейки матки, тесно связанного с анальным и вагинальным раком. Кроме того, интерлейкин-12 (IL-12) индуцирует противоопухолевый иммунитет. Целью данного исследования было изучение роли ВПЧ в РПЖ и определение его влияния на сывороточный уровень IL-12. В период с 2018 по 2022 год в Ахвазе исследователи получили 55 парафинированных образцов злокачественных поражений предстательной железы и 55 контрольных образцов тканей доброкачественной гиперплазии предстательной железы. Образцы крови были взяты у 24 диагностированных онкологических пациентов для оценки уровня IL-12 до начала лечения. Кроме того, в качестве контроля участвовали 24 пациента с доброкачественной гиперплазией предстательной железы. Мы провели экстракцию ДНК фенол-хлороформным методом и исследовали наличие ДНК папилломавируса в тканях с помощью ПЦР в гнездном варианте. Кроме того, в сыворотке крови измеряли уровень IL-12 с помощью ИФА. Результаты не выявили связи между ВПЧ и РПЖ; наличие ВПЧ-инфекции не коррелировало с наличием секреции IL-12. Однако по мере прогрессирования рака уровень IL-12 у пациентов значительно снижался по сравнению с контрольной группой (p < 0,05). ВПЧ может присутствовать в ткани предстательной железы, хотя это не означает, что данный вирус способствует развитию РПЖ. Наиболее значимыми штаммами, инфицирующими ткань предстательной железы, являются типы 16 и 18. По сравнению с контрольной группой и у пациентов с разной суммой баллов по шкале Глисона, уровень секреции интерлейкина-12 достоверно ниже у пациентов с РПЖ. Использование этого важнейшего цитокина позволяет принимать эффективные меры для оценки прогноза, регулирования заболевания или оказания помощи в лечении пациентов.

Prostate cancer (PC); is the second leading cause of cancer mortality among men. Human papillomavirus (HPV) is the most common cause of cervical cancer, strongly associated with anal and vaginal cancers. Also, interleukin-12 (IL-12) induces antitumor immunity. This study aimed to investigate the role of HPV in PC; and determine its effects on serum IL-12. Between 2018 and 2022 in Ahvaz, researchers obtained 55 paraffin samples of malignant prostate lesions and 55 control samples of benign hyperplasia tissues from the prostate. Blood samples were collected from 24 diagnosed cancer patients to assess IL-12 levels before treatment initiation. Additionally, 24 patients with benign prostatic hyperplasia participated as controls. We performed DNA extraction using the phenol-chloroform method and examined the presence of papillomavirus DNA in tissues through Nested-PCR. Subsequently, IL-12 levels in serum were measured using ELISA. The findings did not show the relationship between HPV and PC; HPV infection was not correlated to the presence of IL-12 secretion. However, with the progression of cancer, the level of IL-12 decreased significantly in patients compared to the control group (p < 0.05). HPV infection can exist in prostate tissue, although this does not mean that it contributes to P.C. development. The most significant strains infecting prostate tissue are types 16 and 18. Compared to the control group and with different Gleason scores, prostate cancer patient’s levels of IL-12 secretion are significantly lower. One can make effective measures to assess the prognosis, regulate the condition, or aid in treating individuals using this crucial cytokine.

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The authors declare that there are no conflicts of interest present.