mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-DOC-2939mimmun-2939Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОпределение уровня цитокинов и ростовых факторов для оценки эндотелиальной дисфункции при репродуктивных патологияхDetermination of cytokine and growth factor levels to assess endothelial dysfunction in reproductive pathologieshttps://orcid.org/0000-0001-6087-7152ЧепановС. В.ChepanovS. V.

Чепанов Сергей Владимирович – к.м.н., старший научный сотрудник группы протеомной иммунорегуляции отдела иммунологии и межклеточных взаимодействий 

Санкт-Петербург

Chepanov Sergey V., PhD (Medicine), Senior Researcher, Proteom Immunoregulation Group, Department of Immunology and Intercellular Interactions

St. Petersburg

chepanovsv@gmail.comhttps://orcid.org/0000-0002-3079-6404СиротскаяА. А.SirotskayaA. A.

Сиротская Анастасия Алексеевна – студент 

Санкт-Петербург

Sirotskaya Anastasia A., Student

St. Petersburg

nastya.marinena@ya.ruhttps://orcid.org/0000-0002-5749-2531СоколовД. И.SokolovD. I.

Соколов Дмитрий Игоревич – д.б.н., доцент, заведующий лабораторией межклеточных взаимодействий

 Санкт-Петербург

Sokolov Dmitry I., PhD, MD (Biology), Associate Professor, Head, Laboratory of Intercellular Interactions

St. Petersburg

falcojugger@yandex.ruhttps://orcid.org/0000-0002-6542-5953БеспаловаО. Н.BespalovaO. N.

Беспалова Олеся Николаевна – д.м.н., заместитель директора по научной работе 

Санкт-Петербург

Bespalova Olesya N., PhD, MD (Medicine), Deputy Director for Research

St. Petersburg

iagmail@ott.ruБабаковВ. Н.BabakovV. N.

Бабаков Владимир Николаевич – к.б.н., заведующий лабораторией молекулярной токсикологии и экспериментальной терапии 

г. п. Кузьмоловское, мкр-н Всеволожский, Ленинградская обл.

Babakov Vladimir N., PhD (Biology), Head, Laboratory of Molecular Toxicology and Experimental Therapy

Kuzmolovskoye settlement, Vsevolozhsky microdistrict, Leningrad Region

babakov@gpech.ruРоговскаяН. Ю.RogovskayaN. Yu.

Роговская Надежда Юрьевна – научный сотрудник лаборатории молекулярной токсикологии и экспериментальной терапии 

г. п. Кузьмоловское, мкр-н Всеволожский, Ленинградская обл.

Rogovskaya Nadezhda Yu., Researcher, Laboratory of Molecular Toxicology and Experimental Therapy

Kuzmolovskoye settlement, Vsevolozhsky microdistrict, Leningrad Region

nadin-r@mail.ruhttps://orcid.org/0000-0002-2203-4987ОрловаЕ. С.OrlovaE. S.

Орлова Екатерина Сергеевна – врач – акушер-гинеколог, заведующая акушерским физиологическим отделением 

Санкт-Петербург

Orlova Ekaterina S., Obstetrician-Gynecologist, Head, Obstetric Physiology Department

St. Petersburg

kate-obstetrician@yandex.ruhttps://orcid.org/0000-0003-1560-7529СельковС. А.SelkovS. A.

Сельков Сергей Алексеевич – д.м.н., профессор, заслуженный деятель науки РФ, руководитель отдела иммунологии и межклеточных взаимодействий 

Санкт-Петербург

Selkov Sergey A., PhD, MD (Medicine), Professor, Honored Scientist of the Russian Federation, Head, Department of Immunology and Intercellular Interactions

St. Petersburg

selkovsa@mail.ruФГБНУ «Научно-исследовательский институт акушерства, гинекологии и репродуктологии имени Д.О. Отта»РоссияD. Ott Research Institute of Obstetrics, Gynecology and ReproductologyRussian FederationФГБОУ ВО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения РФРоссияFirst St. Petersburg State I. Pavlov Medical UniversityRussian FederationФГУП «Научно-исследовательский институт гигиены, профпатологии и экологии человека» Федерального медико-биологического агентства РоссииРоссияResearch Institute of Hygiene, Occupational Pathology and Human EcologyRussian Federation202507072025274803822Copyright © Чепанов С.В., Сиротская А.А., Соколов Д.И., Беспалова О.Н., Бабаков В.Н., Роговская Н.Ю., Орлова Е.С., Сельков С.А., 20252025Чепанов С.В., Сиротская А.А., Соколов Д.И., Беспалова О.Н., Бабаков В.Н., Роговская Н.Ю., Орлова Е.С., Сельков С.А.Chepanov S.V., Sirotskaya A.A., Sokolov D.I., Bespalova O.N., Babakov V.N., Rogovskaya N.Y., Orlova E.S., Selkov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/2939

Цель работы – оценить уровень цитокинов и ростовых факторов в периферической крови у женщин с невынашиванием беременности, в том числе на фоне носительства антифосфолипидных антител (АФА). Обследованы 88 женщин в возрасте от 22 до 45 лет. Пациентки были разделены на группы: группа контроля – женщины с физиологически протекавшей беременностью и основная группа – женщины с одним и более случаями невынашивания беременности в анамнезе. Основная группа была подразделена на подгруппы в зависимости от наличия либо отсутствия в сыворотке крови АФА. В свою очередь, подгруппа с наличием АФА и невынашиванием беременности в анамнезе была подразделена на подгруппы с высоким и низким уровнем АФА. В сыворотках и плазме периферической крови определяли уровни антител к кардиолипину и бета-2-гликопротеину-I и концентрацию цитокинов (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, Eotaxin, bFGF, G-CSF, GM-CSF, IFNg, IP-10, MCP-1(MCAF), MIP-1a, PDGF-BB, MIP-1b, RANTES, TNFa, VEGF). Установлено, что женщины с невынашиванием в анамнезе как без АФА, так и с аутоантителами находятся в группе риска в связи с повышением содержания провоспалительных цитокинов в периферической крови; женщины с АФА и привычным выкидышем (ПВ) в анамнезе являются, кроме того, группой, угрожаемой по развитию преэклампсии в связи с повышением VEGF, IL-6 и IL-8 в периферической крови. АФА независимо от уровня могут вносить свой вклад в развитие патологического процесса; у женщин с АФА выявлено нарушение баланса прои противовоспалительных цитокинов за счет снижения компенсаторного противовоспалительного ответа. Установлено, что женщины с АФА и ПВ в анамнезе более подвержены развитию плацентарных нарушений. Определение комплекса иммунологических маркеров позволяет уточнить различные патофизиологические пути реализации акушерской патологии, в том числе невынашивания беременности. Особенно перспективным в этом плане является поиск наиболее значимых сочетаний прои противовоспалительных цитокинов, ростовых, в том числе ангиогенных факторов как с позитивной, так и с негативной регуляцией.

Our objective was to evaluate the level of cytokines and growth factors in peripheral blood in women with pregnancy failure, including women with detectable antiphospholipid antibodies (APhA). Eighty-eight women aged 22 to 45 years were examined. The patients were divided into groups: control group included women with physiologically normal pregnancy; the main group consisted of women with a history of one or more cases of miscarriage. The main group was subdivided into subgroups depending on the presence or absence of APhA in blood serum. The APhA-positive subgroup and a history of miscarriage was then subdivided into subgroups with high and low levels as measured by ELISA test. We evaluated the levels of antibodies to cardiolipin and beta2-glycoprotein-I, and the concentrations of cytokines (IL-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17, Eotaxin, bFGF, G-CSF, GM-CSF, IFNg, IP-10, MCP-1 (MCAF), MIP-1a, PDGF-BB, MIP-1b, RANTES, TNFa, VEGF) in their blood serum and plasma samples. Women with a history of miscarriage, either negative or positive for APhA, are at risk, having an increased contents of proinflammatory cytokines in peripheral blood. Moreover, the women with APhA and a history of miscarriage comprise a group at risk for developing preeclampsia, having increased VEGF, IL-6 and IL-8 levels in peripheral blood. AFAs, regardless of their level, may contribute to development of the pathological process, since an imbalance of proand anti-inflammatory cytokines has been shown in women with APhA, due to a decrease in compensatory anti-inflammatory response. Women with a history of miscarriage and AFAs are more susceptible to placental disorders. Determination of a complex of immunological markers enabled us to clarify various pathophysiological pathways of obstetric pathology, including miscarriage of pregnancy. In this regard, the promising approach would be a search for the most significant combinations of proand anti-inflammatory cytokines, growth-promoting and angiogenic factors with both positive and negative regulation.

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The authors declare that there are no conflicts of interest present.