mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2009-2-3-147-152mimmun-277Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИНДУЦИРОВАННАЯ IFNγ ПРОДУКЦИЯ КИНУРЕНИНА и ЭКСПРЕССИЯ ГЕНА ИНДОЛАМИН 2,3-ДИОКСИГЕНАЗЫ ПРИ ПСОРИАЗЕIFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASISКаутД. А.KautD. A.s.sibiryak@gmail.comЯмидановР. С.YamidanovR. S.s.sibiryak@gmail.comСадовниковС. В.SadovnikovS. V.s.sibiryak@gmail.comКапулерО. М.KapulerO. M.s.sibiryak@gmail.comВахитовВ. А.VakhitovV. A.s.sibiryak@gmail.comСибирякС. В.SibiryakS. V.

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s.sibiryak@gmail.comИнститут биохимии и генетики Уфимского научного центра РАН, г. Уфа; Институт иммунологии и физиологии УрО РАН, г. ЕкатеринбургРоссияИнститут биохимии и генетики Уфимского научного центра РАН, г. УфаРоссияИнститут иммунологии и физиологии УрО РАН, г. ЕкатеринбургРоссия200918072014112-3147152Copyright © Каут Д.А., Ямиданов Р.С., Садовников С.В., Капулер О.М., Вахитов В.А., Сибиряк С.В., 20142014Каут Д.А., Ямиданов Р.С., Садовников С.В., Капулер О.М., Вахитов В.А., Сибиряк С.В.Kaut D.A., Yamidanov R.S., Sadovnikov S.V., Kapuler O.M., Vakhitov V.A., Sibiryak S.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/277

Резюме. Реализуемый индоламин 2,3-диоксигеназой (INDO) альтернативный путь биотрансформации L-триптофана с образованием «кинурениновых» метаболитов играет важнейшую роль в механизмах иммунорегуляции и «негативном» контроле иммунного воспаления. Мононуклеары периферической крови здоровых доноров, больных прогрессирующим вульгарным неосложненным псориазом и псориазом, осложенным артропатией, инкубировали 24 часа в присутствии IFNγ (500 IU/ml, стимулированные культуры) или без цитокина (контрольные культуры). Установлено, что мононуклеары периферической крови больных прогрессирующим псориазом характеризуются изменением спонтанной и индуцированной экспрессии гена индоламин 2,3-диоксигеназы (INDO) и изменением спонтанной и стимулированной продукции кинуренина. При неосложненном псориазе в сравнении со здоровыми донорами спонтанная продукция кинуренина, спонтанная и индуцированная экспрессия INDO в мононуклеарах незначительно нарастают, но индуцированный синтез кинуренина резко увеличен. При псориазе, осложненном артропатией, спонтанная продукция кинуренина и экспрессия INDO снижены, а резкое возрастание индуцированной IFNγ экспрессии гена INDO не сопровождается адекватным нарастанием продукции кинуренина.

Abstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healthy volunteers, patients with progressive vulgar psoriasis (PASI (M±SD) = 25.6±16.6), or patients with psoriatic arthropathy (PASI = 40.3±24.5). The cells were incubated for 24 hrs with IFNγ (500 IU/ml, stimulated cultures) or without cytokine (control cultures). Distinct abnormalities in spontaneous and induced kynurenine production (colorimetric method) and INDO expression (semi-quantitative RT-PCR) were observed in psoriasis and psoriatic arthritis. PBMCs from psoriatic patients, in comparison with healthy donors, were characterized with slightly increased spontaneous kynurenine production, spontaneous and IFNγ-induced INDO expression, while IFNγ-induced kynurenine levels were approximately two times higher. In psoriatic arthritis, spontaneous kynurenine production and INDO expression were significantly lower than in donor’s PBMC, whereas IFNγ-induced kynurenine production were the same as for the donors, while IFNγ – induced INDO expression was markedly increased.

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The authors declare that there are no conflicts of interest present.