mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-6-571-576mimmun-271Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSВЛИЯНИЕ БАКТЕРИАЛЬНЫХ ЛИГАНДОВ ПАТТЕРН-РАСПОЗНАЮЩИХ РЕЦЕПТОРОВ МОНОЦИТОПОДОБНЫХ КЛЕТОК THP-1 НА ИХ ТРАНСЭНДОТЕЛИАЛЬНУЮ МИГРАЦИЮEFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATIONСтариковаЭ. А.StarikovaE. P.

Отдел иммунологии

 

Starickova@yandex.ruСоколовД. И.SokolovD. I.Starickova@yandex.ruБуроваЛ. А.BurovaL. A.Starickova@yandex.ruСельковС. А.Sel’kovS. A.Starickova@yandex.ruФрейдлинИ. С.FreidlinI. S.Starickova@yandex.ruГУ НИИ экспериментальной медицины РАМН, Санкт-ПетербургRussian FederationГУ НИИ акушерства и гинекологии им. Д.О. Отта, Санкт-ПетербургRussian Federation200818072014106571576Copyright © Старикова Э.А., Соколов Д.И., Бурова Л.А., Сельков С.А., Фрейдлин И.С., 20142014Старикова Э.А., Соколов Д.И., Бурова Л.А., Сельков С.А., Фрейдлин И.С.Starikova E.P., Sokolov D.I., Burova L.A., Sel’kov S.A., Freidlin I.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/271

Abstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 strain). Both LPS and lysate of Streptococcus pyogenes acted as chemoattractants for THP-1 cells. he studied components of Streptococcus pyogenes proved to be more active stimulants of transendothelial THP-1 cell migration, than LPS from E. coli. During spontaneous transmigration of THP-1 cells through a monolayer of endothelial cells, augmented levels of chemokines (RANTES, MCP-1, IL-8, IP-10) were noticed, that were more pronounced in presence of LPS. Upon spontaneous transmigration of THP-1 cells through endothelial monolayer, the levels of proinflammatory cytokines (TNFα, IL-1β and IL-6) in cultural medium were found to be rather low. The transmigration-associated secretion of these cytokines increased in presence of LPS and Streptococcus pyogenes lysate. Incubation with these bacterial constituents did increase cytokine levels both in monoculture of THP-1 cells and in transmigration model. Our results suggest that the levels of THP-1 transendothelial migration depend mainly on activation of monocyte-like cells influenced by PRR-ligands from Streptococcus pyogenes lysate. (Med. Immunol., vol. 10, N 6, pp 571-576).

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The authors declare that there are no conflicts of interest present.