mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-6-563-570mimmun-269Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESРАЗРАБОТКА КОМПЛЕКСА ТЕСТ-СИСТЕМ НА ОСНОВЕ ОТ-ПЦР В РЕЖИМЕ РЕАЛЬНОГО ВРЕМЕНИ ДЛЯ ОПРЕДЕЛЕНИЯ ЦИТОКИНОВОГО ПРОФИЛЯ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ КРОВИ И СИНОВИАЛЬНОЙ ЖИДКОСТИ ПРИ РЕВМАТОИДНОМ АРТРИТЕDEVELOPMENT OF A REAL-TIME RT-PCR-BASED TEST PANEL FOR ASSESSMENT OF CYTOKINE PROFILE IN MONONUCLEAR CELLS FROM BLOOD AND SYNOVIAL FLUID IN RHEUMATOID ARTHRITISТрофимовД. Ю.TrofimovD. Yu.irramnlab@rambler.ruБурменскаяО. В.BurmenskayaO. V.irramnlab@rambler.ruБатеневаЕ. И.BatenevaE. I.irramnlab@rambler.ruАлексеевЛ. П.AlexeevL. P.irramnlab@rambler.ruНасоновЕ. Л.NasonovE. L.irramnlab@rambler.ruАлександроваЕ. Н.AlexandrovaE. N.

115522, Москва, Каширское шоссе, 34а

irramnlab@rambler.ruНовиковА. А.NovikovA. A.irramnlab@rambler.ruЗАО «НПФ ДНК-Технология»Russian FederationГУ Институт ревматологии РАМН, МоскваRussian Federation200818072014106563570Copyright © Трофимов Д.Ю., Бурменская О.В., Батенева Е.И., Алексеев Л.П., Насонов Е.Л., Александрова Е.Н., Новиков А.А., 20142014Трофимов Д.Ю., Бурменская О.В., Батенева Е.И., Алексеев Л.П., Насонов Е.Л., Александрова Е.Н., Новиков А.А.Trofimov D.Y., Burmenskaya O.V., Bateneva E.I., Alexeev L.P., Nasonov E.L., Alexandrova E.N., Novikov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/269

Abstract. An imbalance between pro- and anti-inflammatory cytokine synthesis plays a crucial role in pathogenesis of rheumatoid arthritis (RA), including erosive joint lesions. Differences in cytokine profiles of RA patients are sufficiently investigated. However, possible interrelations between cytokine profile, immune inflammation, RA progression, and the disease prognosis require further investigations. Due to active search for novel targets in anti-cytokine therapy of RA, evaluation of cytokine levels in peripheral blood and synovial joint fluid remain quite relevant. Therefore, novel methods aimed to determine mRNA expressed by cytokine genes are thought to be promising. Our research was intended to develop test systems for quantitative determination of mRNAs for the following cytokines: TNFα, IL-1β, IL-6, IL-8, IL-17, IL-15, IL-10, IFNγ, IL-4, IL-2. Present article concerns specificities of the developed test systems, description of their technical principles, as well as comparative studies of cytokine gene expression in peripheral blood and synovial joint fluid in RA patients. (Med. Immunol., 2008, vol. 10, N 6, pp 563-570).

ОТ-ПЦРцитокиновый профильмононуклеарные клеткиревматоидный артритTNFαIL-1βIL-6IL-8IL-17IL-15IL-10IFNγIL-4IL-2RT-PCRcytokine profilemononuclear cellsrheumatoid arthritisTNFαIL-1βIL-6IL-8IL-17IL-15IL-10IFNγIL-4IL-2ReferencesНасонов E.Л., Гукасян Д.А., Насонова М.Б. Иммунопатология ревматоидного артрита и остеопороз: новые данные // Остеопороз и остеопатии. – 2000. – № 2. – С. 4-7.Насонов E.Л., Гукасян Д.А., Насонова М.Б. Иммунопатология ревматоидного артрита и остеопороз: новые данные // Остеопороз и остеопатии. – 2000. – № 2. – С. 4-7.Abbas A.K. Murphy K.M., Sher A. Functional diversity of T helper lymphocytes // Nature. – 1996. – Vol. 383. – P. 787-793.Abbas A.K. Murphy K.M., Sher A. Functional diversity of T helper lymphocytes // Nature. – 1996. – Vol. 383. – P. 787-793.Arnett F.C., Edworthy S.M., Bloch D.A., McShane D.J., Fries J.F., Cooper N.S., Healy L.A., Kaplan S.R., Liang M.H., Luthra H.S. at al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis // Arthritis Rheum. – 1988. – Vol. 31. – P.315-324.Arnett F.C., Edworthy S.M., Bloch D.A., McShane D.J., Fries J.F., Cooper N.S., Healy L.A., Kaplan S.R., Liang M.H., Luthra H.S. at al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis // Arthritis Rheum. – 1988. – Vol. 31. – P.315-324.Dolhain R.J., van der Heiden A.N., ter Haar N.T. Breedveld F.C., Miltenburg A.M. Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patient with rheumatoid arthritis // Arthritis Rheum. – 1996. – Vol. 39. – P. 1961-1969.Dolhain R.J., van der Heiden A.N., ter Haar N.T. Breedveld F.C., Miltenburg A.M. Shift toward T lymphocytes with a T helper 1 cytokine-secretion profile in the joints of patient with rheumatoid arthritis // Arthritis Rheum. – 1996. – Vol. 39. – P. 1961-1969.Gracie J.A., Forsey R.J., Chan W.L., Gilmour A., Leung B.P., Greer M.R., Kennedy K., Carter R., Wei X.Q., Xu D., Field M., Foulis A., Liew F.Y., McInnes I.B. A proinflammatory role for IL-18 in rheumatoid arthritis // J Clin Invest. – 1999. – Vol. 104. – P. 1393 - 1401.Gracie J.A., Forsey R.J., Chan W.L., Gilmour A., Leung B.P., Greer M.R., Kennedy K., Carter R., Wei X.Q., Xu D., Field M., Foulis A., Liew F.Y., McInnes I.B. A proinflammatory role for IL-18 in rheumatoid arthritis // J Clin Invest. – 1999. – Vol. 104. – P. 1393 - 1401.Gaffen S.L. Biology of recently discovered cytokines: Interleukin-17 – a unique inflammatory cytokine with roles in bone biology and arthritis // Arthritis Res Ther. – 2004. – Vol. 6. – P. 240-247.Gaffen S.L. Biology of recently discovered cytokines: Interleukin-17 – a unique inflammatory cytokine with roles in bone biology and arthritis // Arthritis Res Ther. – 2004. – Vol. 6. – P. 240-247.Kim K-W., Cho M-L, Park M-K., Yoon C.H., Park S.H., Lee S.H., Kim H.Y. Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor kB-dependent pathway in patients with rheumatoid arthritis // Arthritis Res Ther. – 2005. – Vol. 7.– P. 139-148.Kim K-W., Cho M-L, Park M-K., Yoon C.H., Park S.H., Lee S.H., Kim H.Y. Increased interleukin-17 production via a phosphoinositide 3-kinase/Akt and nuclear factor kB-dependent pathway in patients with rheumatoid arthritis // Arthritis Res Ther. – 2005. – Vol. 7.– P. 139-148.McInnes I.B., Gracie J.A., Harnett M., Harnett W., Liew F.Y. New strategies to control inflammatory synovitis: interleukin 15 and beyond // Ann Rheum Dis. – 2003. – Vol. 62. – P. 51-54.McInnes I.B., Gracie J.A., Harnett M., Harnett W., Liew F.Y. New strategies to control inflammatory synovitis: interleukin 15 and beyond // Ann Rheum Dis. – 2003. – Vol. 62. – P. 51-54.McInnes I.B., Gracie J.A. Interleukin 15: a proinflammatory role in rheumatoid arthritis synovitis // Immunol Today. – 1998. – Vol. 19. – P. 75-79.McInnes I.B., Gracie J.A. Interleukin 15: a proinflammatory role in rheumatoid arthritis synovitis // Immunol Today. – 1998. – Vol. 19. – P. 75-79.Miosses P.,van den Berg W. Th1/Th2 cytokine balance inarthritis // Arthritis Rheum. – 1997. – Vol. 40. – P. 2105-2115.Miosses P.,van den Berg W. Th1/Th2 cytokine balance inarthritis // Arthritis Rheum. – 1997. – Vol. 40. – P. 2105-2115.Morita Y., Yamamura M., Kawashima M. Harada S., Tsuji K., Shibuya K., Maruyama K., Makino H. Flow cytometric singl-cell analisis of cytokine production by CD4+ T cell in synovial tissue and periferal blood from patients with rheumatoid arthritis // Arthritis Rheum. – 1998. – Vol. 41. – P. 1669 - 1676.Morita Y., Yamamura M., Kawashima M. Harada S., Tsuji K., Shibuya K., Maruyama K., Makino H. Flow cytometric singl-cell analisis of cytokine production by CD4+ T cell in synovial tissue and periferal blood from patients with rheumatoid arthritis // Arthritis Rheum. – 1998. – Vol. 41. – P. 1669 - 1676.Park J.Y., Pillinger M.H. Interleukin-6 the pathogenesis of rheumatoid arthritis // Bull NYU Hosp Joint Dis. – 2007. – Vol. 65. – P. 4-10.Park J.Y., Pillinger M.H. Interleukin-6 the pathogenesis of rheumatoid arthritis // Bull NYU Hosp Joint Dis. – 2007. – Vol. 65. – P. 4-10.Simon A.K., Seipelt E., Sieper J. Divergent T-cell cytokine patterns in iflammatory arthritis // Proc Natl Acad Sci USA. – 1994. – Vol. 91. – P. 8562 - 8566.Simon A.K., Seipelt E., Sieper J. Divergent T-cell cytokine patterns in iflammatory arthritis // Proc Natl Acad Sci USA. – 1994. – Vol. 91. – P. 8562 - 8566.Van Roon J.A., van Roy J.L.,Duits A., Lafeber F.P., Bijlsma J.W. Proinflammatory cytokine production and cartilage damage due to rheumatoid synovial T-helper-1 activition is inhibited by interleukin-4 // Ann Rheum Dis. – 1995. – Vol.54. – P. 836-840.Van Roon J.A., van Roy J.L.,Duits A., Lafeber F.P., Bijlsma J.W. Proinflammatory cytokine production and cartilage damage due to rheumatoid synovial T-helper-1 activition is inhibited by interleukin-4 // Ann Rheum Dis. – 1995. – Vol.54. – P. 836-840.

The authors declare that there are no conflicts of interest present.