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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">mimmun</journal-id><journal-title-group><journal-title xml:lang="ru">Медицинская иммунология</journal-title><trans-title-group xml:lang="en"><trans-title>Medical Immunology (Russia)</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1563-0625</issn><issn pub-type="epub">2313-741X</issn><publisher><publisher-name>SPb RAACI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.15789/1563-0625-IOS-2274</article-id><article-id custom-type="elpub" pub-id-type="custom">mimmun-2440</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МАТЕРИАЛЫ ФОРУМА "ДНИ ИММУНОЛОГИИ В СПБ" 2021</subject></subj-group></article-categories><title-group><article-title>ВЛИЯНИЕ СУБКЛИНИЧЕСКОГО КЕТОЗА КОРОВ НА ФОРМИРОВАНИЕ КОЛОСТРАЛЬНОГО ИММУНИТЕТА ТЕЛЯТ</article-title><trans-title-group xml:lang="en"><trans-title>INFLUENCE OF SUBCLINICAL KETOSIS IN COWS ON FORMATION OF COLOSTRAL IMMUNITY IN CALVES</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Васильева</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vasilieva</surname><given-names>S. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.вет.н., доцент, доцент кафедры биохимии и физиологии,</p><p>196084, Санкт-Петербург, ул. Черниговская, 5</p></bio><bio xml:lang="en"><p>PhD (Veterinary medicine), Associate Professor, Department of Biochemistry and Physiology, </p><p>196084, St. Petersburg, Chernigovskaya str., 5</p></bio><email xlink:type="simple">svvet@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Васильев</surname><given-names>Р. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Vasiliev</surname><given-names>R. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>к.вет.н., доцент, доцент кафедры клинической диагностики,</p><p>196084, Санкт-Петербург, ул. Черниговская, 5</p></bio><bio xml:lang="en"><p>PhD (Veterinary medicine), Associate Professor, Department of Department of Clinical Diagnostics,</p><p>196084, St. Petersburg, Chernigovskaya str., 5</p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">ФГБОУ ВО «Санкт-Петербургский государственный университет ветеринарной медицины»<country>Россия</country></aff><aff xml:lang="en">St. Petersburg State University of Veterinary Medicine<country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>19</day><month>10</month><year>2021</year></pub-date><volume>23</volume><issue>4</issue><fpage>981</fpage><lpage>986</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Васильева С.В., Васильев Р.М., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Васильева С.В., Васильев Р.М.</copyright-holder><copyright-holder xml:lang="en">Vasilieva S.V., Vasiliev R.M.</copyright-holder><license license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.mimmun.ru/mimmun/article/view/2440">https://www.mimmun.ru/mimmun/article/view/2440</self-uri><abstract><p>Здоровье новорожденного всецело зависит от состояния организма матери на протяжении всей беременности. Обеспечение оптимальных условий содержания беременных животных базируется, в первую очередь, на полноценном кормлении и обеспечении санитарно-гигиенических условий окружающей среды. Большую нагрузку организм коровы претерпевает в транзитный период, который начинается за 3 недели до отела и продолжается в течение шести недель. При нарушении технологии кормления и содержания у коров в данный период часто возникают метаболические нарушения, которые проявляются повышенной выработкой кетонов. Известно, что становление иммунитета в ранний постнатальный период у теленка во многом зависит от своевременной выпойки молозива. Материнские иммуноглобулины из молозива попадают непосредственно в системный кровоток новорожденного в тонком отделе кишечника по тубулярной системе эпителиальных клеток путем пиноцитоза. Задача исследования – изучить влияние субклинического кетоза у коров-матерей на формирование колострального иммунитета у родившихся от них телят. Для исследования были отобраны стельные коровы 3-6 лет за 3-7 дней до родов. У коров были взяты пробы мочи и крови. С целью выявления субклинического кетоза у коров было проведено исследование мочи на содержание кетонов. По результатам исследования были сформированы две группы по 10 голов – в первой группе (подопытной) уровень кетоновых тел в моче составил от 1,8 до 3,7 ммоль/л, во второй группе (контрольной) кетоны в моче не обнаружены. Сразу после отела у коров отбирали порции молозива, а у новорожденных телят брали кровь через сутки после первой выпойки молозива. В обезжиренном молозиве и в сыворотке крови новорожденных телят исследовали содержание иммуноглобулинов. В сыворотке крови суточных телят также определяли содержание общего белка биуретовым методом, альбуминов – фотометрическим методом с бромкрезоловым зеленым.</p><p>По результатам проведенного исследования было обнаружено снижение классов иммуноглобулинов G, M и A в сыворотке крови коров перед отелом на 19,1-23,5%, в молозиве – на 23,7-34,4% и в сыворотке крови суточных телят – на 21,7-27,6%. В наибольшей степени определялось снижение концентрации IgM. На содержание в крови телят альбуминов субклинический кетоз коров-матерей практически не оказывал влияние. </p></abstract><trans-abstract xml:lang="en"><p>The health of the newborn depends entirely on the state of the mother’s body throughout the pregnancy. Ensuring optimal conditions for keeping pregnant animals is based, first of all, on adequate feeding and ensuring the sanitary and hygienic conditions of the environment. The cow’s body undergoes a great load during the transition period, which begins 3 weeks before calving and lasts for six weeks. When the technology of feeding and housing is violated, during this period, metabolic disorders often occur in cows, which are manifested by increased production of ketones. It is known that the development of immunity in the early postnatal period in a calf largely depends on the timely feeding of colostrum. Maternal immunoglobulins from colostrum enter the systemic circulation of the newborn in the small intestine through the tubular system of epithelial cells by pinocytosis.</p><p>The aim of the study is to study the effect of subclinical ketosis in mothers cows on the formation of colostral immunity in calves born from them.</p><p>For the study, pregnant cows 3-6 years old were selected 3-7 days before delivery. Urine and blood samples were taken from the cows. In order to identify subclinical ketosis in cows, urine was tested for ketones. According to the results of the study, two groups of 10 animals were formed – in the first group (experimental) the level of ketone bodies in the urine ranged from 1.8 to 3.7 mmol/l, in the second group (control) ketones were not found in the urine. Immediately after calving, portions of colostrum were taken from the cows, and blood was taken from newborn calves a day after the first colostrum was fed. The content of immunoglobulins was studied in skim colostrum and in the blood serum of newborn calves. In the blood serum of day-old calves, the content of total protein was also determined by the biuret method, albumin – by the photometric method with bromcresol green.</p><p>According to the results of the study, a decrease in the classes of immunoglobulins G, M and A was found in the blood serum of cows before calving by 19.1-23.5%, in colostrum – by 23.7-34.4%, and in the blood serum of day old calves – by 21.7-27.6%. The decrease in IgM concentration was determined to the greatest extent. Subclinical ketosis of mothers had practically no effect on the content of albumin in the blood of calves. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>коровы</kwd><kwd>телята</kwd><kwd>иммуноглобулины</kwd><kwd>молозиво</kwd><kwd>колостральный иммунитет</kwd><kwd>кетоз</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cows</kwd><kwd>calves</kwd><kwd>immunoglobulins</kwd><kwd>colostrum</kwd><kwd>colostral immunity</kwd><kwd>ketosis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Bakaeva L.N., Karamaeva A.S., Karamaev S.V. Development of immune status in newborn calves of dairy and combined breeds. Dairy and Beef Cattle Breeding, 2019, no. 1, pp. 32-36. (In Russ.)</mixed-citation><mixed-citation xml:lang="en">Bakaeva L.N., Karamaeva A.S., Karamaev S.V. 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