mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-EOE-2379mimmun-2379Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВлияние экстрактов микроводорослей на уровни цитокинов у мышей-самок C57Bl6Effect of extracts from microalgae on cytokine levels in female C57Bl6 miceЛыковА. П.LykovA. P.

Лыков Александр Петрович – кандидат медицинских наук, ведущий научный сотрудник.

630060, Новосибирск, ул. Тимакова, 2

Тел.: 8 (383) 335-93-32

Alexander P. Lykov - PhD (Medicine), Leading Research Associate, Research Institute of Clinical and Experimental Lymphology, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences.

2 Timakov St Novosibirsk 630060

Phone: +7 (383) 335-93-32

aplykov2@mail.ruУваровИ. П.UvarovI. P.

Руководитель ГБУ Новосибирской области «Управление ветеринарии города Новосибирска».

Новосибирск

Novosibirsk Regional Department of Veterinary Medicine.

Novosibirsk

ГеворгизР. Г.GevorgizR. G.

Кандидат биологических наук, старший научный сотрудник отдела аквакультур и морской фармакологии.

Севастополь

PhD (Biology), Senior Research Associate, Department of the Aquaculture and Sea Pharmacology, A. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences.

Sevastopol

ЖелезноваС. Н.ZheleznovaS. N.

Младший научный сотрудник отдела аквакультур и морской фармакологии.

Севастополь

Junior Research Associate, Department of the Aquaculture and Sea Pharmacology, A. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences.

Sevastopol

Научно-исследовательский институт клинической и экспериментальной лимфологии – филиал Федерального исследовательского центра “Институт цитологии и генетики” Сибирского отделения Российской академии наук»РоссияResearch Institute of Clinical and Experimental Lymphology, Institute of Cytology and Genetics, Siberian Branch, Russian Academy of SciencesRussian FederationУправление ветеринарии города НовосибирскаРоссияDepartment of Veterinary Medicine of the city of NovosibirskRussian FederationИнститут биологии южных морей имени А.О. Ковалевского Российской академии наукРоссияA. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of SciencesRussian Federation2023060220232518190Copyright © Лыков А.П., Уваров И.П., Геворгиз Р.Г., Железнова С.Н., 20232023Лыков А.П., Уваров И.П., Геворгиз Р.Г., Железнова С.Н.Lykov A.P., Uvarov I.P., Gevorgiz R.G., Zheleznova S.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/2379

Микроводоросли пресноводных водоемов и морей представляют собой ценный источник широкого спектра биологически активных веществ, способных влиять на  клетки  иммунной  системы и их функциональный статус. Цитокины вовлечены во все циклы жизни клеток организма (пролиферация, созревание, дифференцировка, апоптоз/некроз). Проведено исследование влияния приема в пищу стандартного корма для лабораторных животных, обогащенных масляным экстрактом микроводорослей различных систематических групп на уровни цитокинов в сыворотке крови, кондиционных средах иммуноцитов и ткани почек и печени. Стандартный корм пропитывали масляными экстрактами микроводорослей (C. vulgaris, Coelastrella sp., A. platensis, C. closterium и P. purpureum), в контроле корм пропитывали чистым растительным маслом, животные принимали корм    в течение 12 дней. Забирали кровь, селезенку и тимус для выделения иммуноцитов, почки и печень для получения экстракта в диметилсульфоксиде. Кондиционные среды спленоцитов и тимоцитов получали добавлением конканавалина А (0 и 10 мкг/мл). В сыворотке, кондиционных средах, экстрактах исследовали уровень NO, IL-1β, IL-10, TNFα и NO. В сыворотке выявлено влияние микроводорослей на уровень IL-1β и TNFα. Активация иммуноцитов в опытных группах вела к изменению уровня продукции IL-1β, TNFα и IL-10. Отмечено изменение уровня цитокинов, NO в экстрактах печени и почек в опытных группах.

Таким образом, экстракты микроводорослей различных систематических групп оказывают влияние на уровни цитокинов в сыворотке крови, кондиционных средах спленоцитов и тимоцитов, тканях почек и печени.

Microalgae from the freshwater basins and seas are a valuable source of broad-spectrum biologically active substances that can affect the cells of immune system and their functional state. Cytokines are involved in all vital processes proceeding in the living cells (proliferation, maturation, differentiation, apoptosis/ necrosis). A study was performed in order to assess the effects of standard food formula for experimental animals supplemented with oil extract of microalgae from various systematic groups upon the levels of cytokines in blood serum, culture media conditioned by immunocytes, as well as kidney and liver tissues. The standard food was impregnated in oil extracts of microalgae (C. vulgaris, Coelastrella sp., A. platensis, C. closterium, and P. purpureum). In control series, the food was impregnated with pure vegetable oil. The animals were fed these foods for 12 days. Blood, spleen and thymus were taken to isolate immunocytes, kidneys and liver, and dimethyl sulfoxide extracts of the cells were produced. The conditioned media of splenocytes and thymocytes were obtained by adding concanavalin A (0 and 10 μg/ml) to the cultured cells. The levels of NO, IL-1β, IL-10, TNFα, and NO were determined in serum, conditioned media, and tissue extracts. In serum, the influence of microalgae on the levels of IL-1β and TNFα was revealed. Activation of immunocytes in experimental groups was followed by changes in IL-1β, TNFα and IL-10 production. Changes of cytokine and NO levels were revealed in liver and kidney extracts in experimental groups. Thus, microalgae extracts of various systematic groups affect the levels of cytokines in blood serum, cultural media conditioned by splenocytes and thymocytes, kidney and liver tissues.

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The authors declare that there are no conflicts of interest present.