mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2014-1-53-60mimmun-23Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭСТРИОЛ В МОДУЛЯЦИИ ФУНКЦИОНАЛЬНОЙ АКТИВНОСТИ РЕГУЛЯТОРНЫХ ЛИМФОЦИТОВ ПРИ РАССЕЯННОМ СКЛЕРОЗЕESTRIOL IN FUNCTIONAL MODULATION OF REGULATORY LYMPHOCYTES IN MULTIPLE SCLEROSISНекрасоваИ. В.NekrasovaI. V.

к.б.н., научный сотрудник, лаборатория иммунорегуляции

PhD (Biology), Research Associate, Laboratory of Immune Regulation

nirina5@mail.ruШиршевС. В.ShirshevS. V.

д.м.н., профессор, заведующий лабораторией иммунорегуляции

PhD, MD (Medicine), Professor, Head, Laboratory of Immune Regulation

nirina5@mail.ruДанченкоИ. Ю.DanchenkoI. Yu.

заочный аспирант кафедры неврологии лечебного факультета Пермской государственной медицинской академииим. академика Е.А. Вагнера МЗ РФ

Postgraduate Student, Perm State Medical Academy

nirina5@mail.ruФГБУН «Институт экологии и генетики микроорганизмов» УрО РАН, г. ПермьРоссияInstitute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, PermRussian FederationПермская государственная медицинская академия им. академика Е.А.Вагнера МЗ РФ, г. ПермьРоссияPerm State Medical Academy, PermRussian Federation2014070720141615360Copyright © Некрасова И.В., Ширшев С.В., Данченко И.Ю., 20142014Некрасова И.В., Ширшев С.В., Данченко И.Ю.Nekrasova I.V., Shirshev S.V., Danchenko I.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/23

Исследовано влияние эстриола (Е3) в концентрациях, соответствующих I и III триместрам беременности, на уровень функционально активных Т-регуляторных (Treg) и Th17-продуцирующих (Th17) лимфоцитов, а также продукцию соответствующих цитокинов клетками больных рассеянным склерозом (РС) в сравнении со здоровыми донорами. Установлено, что уровень CD4+FoxP3+CTLA-4+(Treg) лимфоцитов пациентов с РС был изначально ниже, чем у здоровых доноров. Процент CD4+RORγt+IL-17A+ (Th17) лимфоцитов статистически достоверно не отличался от такового у здоровых доноров, но имел тенденцию к увеличению. Исходные уровни цитокинов IL-17 и IL-6 в супернатантах клеточных культур больных РС были выше, чем у здоровых доноров, а концентрацияпротивовоспалительного цитокина IL-10, напротив, понижена. Под влиянием Е3 процент Treg увели чивался, а Th17 – снижался. Причем данные эффекты гормона реализуются как на клетках здоровых доноров, так и больных РС. При этом Е3 стимулировал продукцию IL-10 и угнетал секрецию IL-6 и IL-17. Таким образом, наблюдаемое снижение проявлений РС при беременности может быть обусловлено влиянием Е3, что открывает перспективы для дальнейшего использования данного гормона в комплексной терапии РС.

We studied effects of estriol (E3), at the doses corresponding to the I and III trimesters of pregnancy,upon expression of functional markers on T-regulatory (Treg) and Th17-producing (Th17) lymphocytes, aswell as the production of relevant cytokines by the cells of patients with multiple sclerosis (MS), as comparedwith healthy donors. It was found that the levels of CD4+FoxP3+CTLA-4+ (Treg) lymphocytes in MS patientswere initially lower than those of healthy donors. Percentages of CD4+RORγt+IL-17A+ (Th17) lymphocytesdid not significantly differ from those in healthy donors, still showing a tendency for increase. Baseline levelsof IL-17 and IL-6 in cell culture supernates of MS patients were higher than in healthy donors, along withreduced contents of the anti-inflammatory IL-10 cytokine. Percentage of Tregs was increased under theinfluence of estriol, whereas Th17 proportion was diminished. These hormonal effects upon lymphocyteswere reproducible both for healthy donors, and MS patients. Moreover, estriol stimulated IL-10 productionand inhibited secretion of IL-6 and IL-17. Therefore, a sufficient release of MS symptoms observed duringpregnancy may be explained by influence of estriol, thus extending opportunities for rational use of this steroidhormone in MS treatment

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The authors declare that there are no conflicts of interest present.