mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-4-5-463-466mimmun-219Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSАНАЛИЗ КОНТРОЛЬНЫХ ПОКАЗАТЕЛЕЙ «РЕПЛИКАТЫ Т-КЛЕТОК» И «СУММА Т-КЛЕТОК» ПРИ ИССЛЕДОВАНИИ ИММУННОГО СТАТУСА У ВИЧ-ИНФИЦИРОВАННЫХ БОЛЬНЫХ И ВЫЯВЛЕНИЕ ДУБЛЬ-НЕГАТИВНЫХ СУБПОПУЛЯЦИЙ Т-ЛИМФОЦИТОВ (CD45+CD3+CD4–CD8–)ANALYSIS OF CONTROL MEASUREMENTS OF «T-CELL REPLICATES» AND «T-CELL SUMMARY» IN INVESTIGATION OF THE IMMUNE STATUS OF HIV-INFECTED PATIENTS AND DETECTION DOUBLE NEGATIVE SUBPOPULATION OF T-LYMPHOCYTES (CD45+CD3+CD4–CD8–)ШестаковаЕ. В.ShestakovaE. V.khsv@mail.ibch.ruЗурочкаА. В.ZurochkaA. V.khsv@mail.ibch.ruКвятковская С. В.KviatkovskayaS. V.khsv@mail.ibch.ruБецковС. Г.BetskovS. G.khsv@mail.ibch.ruДукардтВ. В.DukardtV. V.khsv@mail.ibch.ruХайдуковС. В.HaidukovS. V.

117997, Москва, ул. Миклухо-Маклая, 16/10

khsv@mail.ibch.ruКлиника ГОУ ВПО Челябинской государственной медицинской академии, г. ЧелябинскРоссияГОУ ВПО Челябинская государственная медицинская академия, г. ЧелябинскРоссияИнститут биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, МоскваРоссия200817072014104-5463466Copyright © Шестакова Е.В., Зурочка А.В., Квятковская  С.В., Бецков С.Г., Дукардт В.В., Хайдуков С.В., 20142014Шестакова Е.В., Зурочка А.В., Квятковская  С.В., Бецков С.Г., Дукардт В.В., Хайдуков С.В.Shestakova E.V., Zurochka A.V., Kviatkovskaya S.V., Betskov S.G., Dukardt V.V., Haidukov S.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/219

Abstract. We analyzed efficiency of application for analytic control parameters, i.e., «T-cell replicates» and «T-cell summary» in a sample of 174 HIV-infected patients, independently of age or disease stage. The patients were divided in two groups. Group 1 consisted of patients with total CD45+CD3+CD4+ and CD45+CD3+CD8+ lymphocyte concentrations being similar to relative and absolute concentrations of CD45+CD3+ lymphocytes, or the difference between absolute measurements not exceeding «T-cell replicates» differences. Group 1 included 36.78% of the whole cohort. Group 2 consisted of patients with total CD45+CD3+CD4+ & CD45+CD3+CD8+ lymphocytes concentrations being lower than relative and absolute concentrations of CD45+CD3+ lymphocytes, and the differences in absolute values being higher than «T-cell replicates» differences (41.38% of total sample). Such difference between control sums may be connected with high concentrations of double-negative γδ-T-cells (CD45+CD3+CD4–CD8–) in the specimens under study. (Med. Immunol., 2008, vol. 10, N 4-5, pp 463-466).

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The authors declare that there are no conflicts of interest present.