mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2014-1-35-42mimmun-20Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESТРАНСКРИПЦИОННЫХ ФАКТОРОВ PAX-5 И STAT6 В ПАТОГЕНЕЗЕ АЛЛЕРГИЧЕСКОЙ БРОНХИАЛЬНОЙ АСТМЫINTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMAМинеевВ. Н.MineevV. N.

д.м.н., профессор кафедры госпитальнойтерапии имени академика М.В. Черноруцкого

PhD, MD (Medicine), Professor,M.V. Chernorutsky Department of Hospital Therapy

vnmineev@mail.ruСорокинаЛ. Н.SorokinaL. N.

д.м.н., доцент кафедры госпитальнойтерапии имени академика М.В. Черноруцкого

PhD, MD (Medicine), AssociateProfessor, M.V. Chernorutsky Department of HospitalTherapy

vnmineev@mail.ruНёмаМ. А.NyomaM. A.

аспирант кафедры госпитальной терапииимени академика М.В. Черноруцкого

PhD Candidate, M.V. ChernorutskyDepartment of Hospital Therapy

vnmineev@mail.ruЕремееваА. В.EremeevaA. V.

аспирант кафедры госпитальнойтерапии имени академика М.В. Черноруцкого

PhD Candidate, M.V. ChernorutskyDepartment of Hospital Therapy

vnmineev@mail.ruСанкт-Петербургский государственный медицинский университет имени академика И.П. Павлова, Санкт-ПетербургРоссияSt. Petersburg State I.P. Pavlov Medical University, St. PetersburgRussian Federation2014050720141613542Copyright © Минеев В.Н., Сорокина Л.Н., Нёма М.А., Еремеева А.В., 20142014Минеев В.Н., Сорокина Л.Н., Нёма М.А., Еремеева А.В.Mineev V.N., Sorokina L.N., Nyoma M.A., Eremeeva A.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/20

The aim of this work was to study an opportunity of coordinated expression of PAX-5 and STAT6 in bronchial asthma (BA) development. 35 healthy controls, 83 patients with allergic bronchial asthma (ABA) and 63 patients with nonallergic bronchial asthma (NABA) o different severity were observed. To evaluate PAX- 5 and β-actin mRNA levels, a reverse transcription-PCR with endpoint detection was applied. To determine STAT6 and pSTAT6 protein levels, ECL Western blotting protocols (Amersham) was used. A negative correlation was revealed between РАХ-5 and STAT6 mRNA expression levels in control group and in ABA. In NABA patients, the appropriate correlations were nonsignificant. Expression of РАХ-5 mRNA in ABA with normal IgE levels showed a significant negative correlation with that of STAT6. Meanwhile, expression levels of active pSTAT6 form in patients with high IgE and eosinophilia did negatively correlate with РАХ-5 mRNA expression. In ABA, effects of IL-4 caused a more significant decrease of РАХ-5 mRNA as compared with those in control group. The strongest negative correlation between РАХ-5 mRNA expression and STAT6 levels was revealed in groups with higher levels of mRNA РАХ-5 and lower levels of STAT6. The revealed negative correlations between STAT6 levels and РАХ-5 mRNA expression suggest that STAT6 may inhibit PAX-5 transcription in ABA group, thus, probably, promoting differentiation of B cells into plasma cells.

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The authors declare that there are no conflicts of interest present.