mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-4-5-379-388mimmun-195Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИЗМЕНЕНИЯ ЭКСПРЕССИИ HLA-DR-АНТИГЕНОВ НА МОНОЦИТАХ У ДЕТЕЙ И ЕЕ КЛИНИЧЕСКАЯ ЗНАЧИМОСТЬ ПРИ СЕПСИСЕCHANGES OF HLA-DR ANTIGEN EXPRESSION ON MONOCYTES IN CHILDREN AND THEIR CLINICAL SIGNIFICANCE IN SEPSISЗурочкаА. В.ZurochkaA. V.khsergey54@mail.ruКотляровА. Н.KotlyarovA. N.khsergey54@mail.ruКувайцевМ. В.KuvaytsevM. V.khsergey54@mail.ruКвятковскаяС. В.KvyatkovskayaS. V.khsergey54@mail.ruЗурочкаВ. А.ZurochkaV. A.khsergey54@mail.ruРябоваЛ. В.RyabovaL. V.khsergey54@mail.ruХайдуковС. В.KhaidukovS. V.

117997, Москва, ул. Миклухо-Маклая, 16/10

khsergey54@mail.ruГОУ ВПО Челябинская государственная медицинская академия, г. ЧелябинскРоссияИнститут биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, МоскваРоссия200817072014104-5379388Copyright © Зурочка А.В., Котляров А.Н., Кувайцев М.В., Квятковская С.В., Зурочка В.А., Рябова Л.В., Хайдуков С.В., 20142014Зурочка А.В., Котляров А.Н., Кувайцев М.В., Квятковская С.В., Зурочка В.А., Рябова Л.В., Хайдуков С.В.Zurochka A.V., Kotlyarov A.N., Kuvaytsev M.V., Kvyatkovskaya S.V., Zurochka V.A., Ryabova L.V., Khaidukov S.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/195

Резюме. Временный иммунодефицит часто связывают с патофизиологическими состояниями, такими как инфаркт миокарда, серьезные травмы или хирургические вмешательства. Однако нет четкого диагностического критерия указывающего на присутствие или отсутствие временного иммунодефицита. Была предложена методика для мониторинга септического состояния пациентов с открытыми травмами и в постоперационный период с использованием метода проточной цитометрии. Принцип оценки септического состояния заключается в измерении экспрессии HLA-DR антигенов на поверхности моноцитов периферической крови. Критерием оценки состояния пациента при сепсисе служит относительное количество моноцитов, экспрессирующих HLA-DR, и благоприятным прогнозом считается, если количество позитивных клеток превышает 40% на 5 день после госпитализации и проведения соответствующей терапии. Этот метод может найти широкое применение для оценки и мониторинга септического состояния пациентов.

Abstract. Temporary immunodeficiency is often associated with various pathological conditions, such as myocardial infarction, severe trauma, or major surgery. However, there are no clinical criterions indicative for presence or absence of such immune deficiency. Meanwhile, clinical signs of infectious complications may be absent because of deficient immune response.

A technique for monitoring septic conditions in the patients with open traumas and during postsurgical period has been proposed, employing a flow-cytometric approach. The evaluation principle for septic conditions consists of measuring expression of HLA-DR antigens at the surface of peripheral blood monocytes.

As a criterion of patient evaluation in septic state, a relative amount of monocytes expressing HLA-DR may be applied, and the disease prognosis is considered as favorable, if the amounts of positive cells exceed 40% by day 5 after the patient was admitted to the hospital, and an adequate treatment was carried out. This technique may find wide application for estimation and monitoring of septic conditions in the patients. (Med. Immunol., vol. 10, N 4-5, pp 379-388).

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The authors declare that there are no conflicts of interest present.