mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2009-1-71-78mimmun-190Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВЛИЯНИЕ СТАТИНОВ И БИОЛОГИЧЕСКИХ ПРЕПАРАТОВ НА АКТИВАЦИЮ МИТОГЕН-АКТИВИРОВАННЫХ ПРОТЕИНКИНАЗ У БОЛЬНЫХ РЕВМАТОИДНЫМ АРТРИТОМEFFECTS OF STATINS AND OTHER BIOLOGICAL PREPARATIONS UPON ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASES IN PATIENTS WITH RHEUMATOID ARTHRITISШиринскийИ. В.ShirinskyI. V.

лаборатория клинической иммунофармакологии

ishirinsky@mail.ruКозловВ. А.KozlovV. A.ishirinsky@mail.ruШиринскийВ. С.ShirinskyV. S.

лаборатория клинической иммунофармакологии

ishirinsky@mail.ruГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскRussian Federation2009170720141117178Copyright © Ширинский И.В., Козлов В.А., Ширинский В.С., 20142014Ширинский И.В., Козлов В.А., Ширинский В.С.Shirinsky I.V., Kozlov V.A., Shirinsky V.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/190

Резюме. Изучалось влияние биологических препаратов для лечения ревматоидного артрита (РА)и статинов на спонтанную и стимулированную активацию МАРК p38 и ERK1/2 в моноцитах больныхРА. Объектом исследования являлись мононуклеары периферической крови (МНК ПК) от больныхc активным РА и здоровых людей. МНК ПК культивировались в присутствии 0; 0,1; 1 и 10 мкмольмевастатина, 10 мкг/мл антагониста рецептора интерлейкина-1 (IL-1Ra), 5 мкг/мл инфликсимабаили 5 мкг/мл растворимого PEGилированного p55-рецептора фактора некроза опухоли альфа (r-met-Hu-sTNF-RI). Для изучения механизмов действия мевастатина на активацию МАРК p38 и ERK1/2в культуры добавлялся L-мевалонат. Клетки окрашивались анти-фосфо-MAPK p38 или анти-фосфо-ERK1/2 и анализировались на проточном цитометре. Было показано, что IL-1Ra и r-met-Hu-sTNF - RI ингибируют спонтанную активацию МАРК р38. Мевастатин уменьшал спонтанное фосфорилирование MAPK p38 и ERK1/2. Мевастатин-индуцированное подавление активации p38 и ERK1/2 не было дозозависимым. L-мевалонат полностью предотвращал мевастатин-индуцированное уменьшение фосфорилирования MAPK р38 и частично отменял ингибирование МАРК ERK1/2. Таким образом, подавление активации МАРК является общим механизмом противовоспалительного действия статинов и некоторых биологических препаратов для лечения РА.

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The authors declare that there are no conflicts of interest present.