mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-4-5-337-346mimmun-184Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭКСПРЕССИЯ мРНК ХЕМОКИНОВ И ХЕМОКИНОВЫХ РЕЦЕПТОРОВ В КОЖЕ БОЛЬНЫХ ПСОРИАЗОМEXPRESSION OF mRNAS FOR CHEMOKINES AND CHEMOKINE RECEPTORS IN THE SKIN FROM PATIENTS WITH PSORIASISБельтюковаА. С.BeltiukovaA. S.

НМЦ по молекулярной медицине

197022, Санкт-Петербург, ул. Л. Толстого, 6/8

СысоевК. А.SyssoevK. A.ИльинаТ. Н.Il’inaT. N.ШемеровскаяТ. Г.ShemerovskayaT. G.ХобейшМ. М.HobeishM. M.МонаховК. Н.MonakhovK. N.ТотолянАрег А.TotolianAreg A.Государственный медицинский университет им. акад. И.П. Павлова, Санкт-ПетербургRussian FederationГУЗ Городская больница № 25 «Городской ревматологический центр», Санкт-ПетербургRussian Federation200817072014104-5337346Copyright © Бельтюкова А.С., Сысоев К.А., Ильина Т.Н., Шемеровская Т.Г., Хобейш М.М., Монахов К.Н., Тотолян А.А., 20142014Бельтюкова А.С., Сысоев К.А., Ильина Т.Н., Шемеровская Т.Г., Хобейш М.М., Монахов К.Н., Тотолян А.А.Beltiukova A.S., Syssoev K.A., Il’ina T.N., Shemerovskaya T.G., Hobeish M.M., Monakhov K.N., Totolian A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/184

Abstract. Some issues in etiology and pathogenesis of psoriasis are poorly studied. Therefore, a search for new potential markers is actual for diagnostics of psoriasis in less clear cases. In this study, an attempt was undertaken to evaluate contribution of some chemokines and appropriate receptors into pathogenesis of psoriasis. The main group consisted of the patients with psoriatic arthritis (n = 20) and psoriasis vulgaris (n = 9). A group of comparison consisted of patients with sclerodermia (n = 4), and a control group was represented by healthy persons (n = 9). The specimens were taken from visually normal and affected skin areas from psoriatic patients obtained by punch biopsy. Expression of the following chemokines was performed: CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES, CCL11/eotaxin, CCL24/eotaxin-2, CXCL8/IL-8 and their receptors (CCR1, CCR3, CCR5, CXCR1, CXCR2). In cases with PASI values < 10, an increased expression of the following genes was revealed for CCL11/eotaxin (p = 0.03), CXCR1 (р = 0.008), CXCR2 (р = 0.0006) in virtually intact skin and affected skin areas, as well as increased gene expression of CCL24/eotaxin 2 (p = 0.009), CCL5/RANTES (p = 0.05) in visually normal skin.

With PASI values of 10 to 20, an increased gene expression was found for CCL11/eotaxin (p = 0.005), CCL24/eotaxin 2 (p = 0.02), CCL5/RANTES (p = 0.01), CXCR1 (р = 0.0009), CXCR2 (р = 0.002) in skin biopsies from visually healthy and affected skin, as well as increased expression CXCL8 (IL-8) (p = 0.005) in visually normal skin. In cases with PASI > 20, an increased expression of CCL11/eotaxin (p = 0.001), CCL24/eotaxin 2 (p = 0.001), CCL3/MIP-1α (р = 0.02), CXCR1 (p = 0.0001), CXCR2 (p = 0.001) was detected in visually healthy skin samples and affected skin of the patients, as well as higher expression of CCL4/MIP-1β (р = 0.03) in affected skin areas. A reverse correlation was revealed between expression of chemokines, i.e., CCL24/eotaxin 2 (r = –0,94, p = 0.005), CCL3/MIP-1α (r = –0,94, p = 0.005), CCL4/MIP-1β (r = –0,85, p = 0.03) and their receptors: ССR5 (r = –0,79, p = 0.005) and CXCR2 (r = –0,94, p = 0.005) in visually normal skin of the patients with psoriasis and PASI values < 10.

A direct correlation was found between expression of mRNAs for CCL11/eotaxin (r = 0.69, p = 0.04) in visually healthy skin from psoriatic patients, and CCR5 (r = 0.82, p = 0.006) in affected skin of the patients with psoriasis and PASI values of 10 to 20. In the group of patients with PASI values over 20, no correlations were detectable. These data allow us of concluding aboutan important contribution of chemokine system to pathogenesis of psoriasis. (Med. Immunol., vol. 10, N 4-5, pp 337-346).

хемокиныхемокиновые рецепторымРНКпсориатический артритпсориазchemokineschemokines receptorsmRNApsoriatic arthritispsoriasisReferencesКоган К.М. Спектр клинических проявлений псориатического артрита: Метод. рекомендации. – М., 2006.Коган К.М. Спектр клинических проявлений псориатического артрита: Метод. рекомендации. – М., 2006.Федосеев Г.Б., Игнатов Ю.Д. Синдромальная диагностика и базисная фармакотерапия заболеваний внутренних органов. Т. 1. – СПб: «Нордмедиздат», 2004. – С. 74-75.Федосеев Г.Б., Игнатов Ю.Д. Синдромальная диагностика и базисная фармакотерапия заболеваний внутренних органов. Т. 1. – СПб: «Нордмедиздат», 2004. – С. 74-75.Wewers M.E., Lowe N.K. A critical review of visual analogue scales in the measurement of clinical phenomena // Res. Nurs. Health. – 1990. – Vol. 13. – P. 227-236.Wewers M.E., Lowe N.K. A critical review of visual analogue scales in the measurement of clinical phenomena // Res. Nurs. Health. – 1990. – Vol. 13. – P. 227-236.de Groot M., Teunissen M.B., Ortonne J.P., Lambert J.R., Naeyaert J.M., Picavet D.I., Arreaza M.G., Simon J.S., Kraan M., Bos J.D., de Rie M.A. Expression of the chemokine receptor CCR5 in psoriasis and results of a randomized placebo controlled trial with a CCR5 inhibitor // Arch. Dermatol. Res. – 2007. – Vol. 299, N 7. – P. 305-313.de Groot M., Teunissen M.B., Ortonne J.P., Lambert J.R., Naeyaert J.M., Picavet D.I., Arreaza M.G., Simon J.S., Kraan M., Bos J.D., de Rie M.A. Expression of the chemokine receptor CCR5 in psoriasis and results of a randomized placebo controlled trial with a CCR5 inhibitor // Arch. Dermatol. Res. – 2007. – Vol. 299, N 7. – P. 305-313.Kang S., Voorhees J.J. Immunopathogenesis // Textbook of psoriasis / Ed. by Van de Kerkhof P. – Oxford: Blackwell Science, 1999. – P. 106-118.Kang S., Voorhees J.J. Immunopathogenesis // Textbook of psoriasis / Ed. by Van de Kerkhof P. – Oxford: Blackwell Science, 1999. – P. 106-118.Nickoloff B.J. The immunologic and genetic basis of psoriasis // Arch. Dermatol. – 1999. – Vol. 135. – P. 1104-1110.Nickoloff B.J. The immunologic and genetic basis of psoriasis // Arch. Dermatol. – 1999. – Vol. 135. – P. 1104-1110.Nomura I., Goleva E., Howell M.D., Hamid Q.A., Ong P.Y., Hall C.F., Darst M.A., Gao B., Boguniewicz M., Travers J.B., Leung D.Y. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes // J. Immunol. – 2003. – Vol. 171, N 6. – P. 3262-3269.Nomura I., Goleva E., Howell M.D., Hamid Q.A., Ong P.Y., Hall C.F., Darst M.A., Gao B., Boguniewicz M., Travers J.B., Leung D.Y. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes // J. Immunol. – 2003. – Vol. 171, N 6. – P. 3262-3269.Godi A. New approaches to psoriasis treatment // Acta Dermatoven. – 2004.– Vol 13, N2. – P. 50-57.Godi A. New approaches to psoriasis treatment // Acta Dermatoven. – 2004.– Vol 13, N2. – P. 50-57.Prinz J.C. Psoriasis vulgaris, a sterile antibacterial skin reaction, mediated by cross-reactive T-cells. An immunological view on the pathophysiology of psoriasis // Clin. Exp. Dermatol. – 2001. – Vol. 26. – P. 326-332.Prinz J.C. Psoriasis vulgaris, a sterile antibacterial skin reaction, mediated by cross-reactive T-cells. An immunological view on the pathophysiology of psoriasis // Clin. Exp. Dermatol. – 2001. – Vol. 26. – P. 326-332.Schlaak J.F., Buslau M., Jochum W., Hermann E., Girndt M., Gallati H., Meyer zum B - schenfelde K.H., Fleischer B. T cells involved in psoriasis vulgaris belong to the Th1 subset // J. Invest. Dermatol. – 1994. – Vol. 102, N 2. – P. 145-149.Schlaak J.F., Buslau M., Jochum W., Hermann E., Girndt M., Gallati H., Meyer zum B - schenfelde K.H., Fleischer B. T cells involved in psoriasis vulgaris belong to the Th1 subset // J. Invest. Dermatol. – 1994. – Vol. 102, N 2. – P. 145-149.Turka L.A., Goodman R.E., Rutkowski J.L., Sima A.A., Merry A., Mitra R.S., Wrone-Smith T., Toews G., Strieter R.M., Nickoloff B.J. Interleukin 12: a potential link between nerve cells and the immune response in inflammatory disorders // Mol. Med. – 1995. – Vol. 1, N 6. – P. 690-699.Turka L.A., Goodman R.E., Rutkowski J.L., Sima A.A., Merry A., Mitra R.S., Wrone-Smith T., Toews G., Strieter R.M., Nickoloff B.J. Interleukin 12: a potential link between nerve cells and the immune response in inflammatory disorders // Mol. Med. – 1995. – Vol. 1, N 6. – P. 690-699.Yawalkar N., Shrikhande M., Hari Y., Niev-ergelt H., Braathen L.R., Pichler W.J. Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug-induced cutaneous eruptions // J. Allergy Clin. Immunol. – 2000. – Vol. 106, N 6. – P. 1171-1176.Yawalkar N., Shrikhande M., Hari Y., Niev-ergelt H., Braathen L.R., Pichler W.J. Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug-induced cutaneous eruptions // J. Allergy Clin. Immunol. – 2000. – Vol. 106, N 6. – P. 1171-1176.Ghoreschi K., Weigert C., Röcken M. Immunopathogenesis and role of T cells in psoriasis // Clin. Dermatol. – 2007. – Vol. 25, N 6. – P. 574-580.Ghoreschi K., Weigert C., Röcken M. Immunopathogenesis and role of T cells in psoriasis // Clin. Dermatol. – 2007. – Vol. 25, N 6. – P. 574-580.Macchioni P., Boiardi L., Meliconi R., Pulsatelli L., Maldini M.C., Ruggeri R., Facchini A., Salvarani C. Serum chemokines in patients with psoriatic arthritis treated with cyclosporin A // J. Rheumatol. – 1998. – Vol. 25, N 2. – P. 320-325.Macchioni P., Boiardi L., Meliconi R., Pulsatelli L., Maldini M.C., Ruggeri R., Facchini A., Salvarani C. Serum chemokines in patients with psoriatic arthritis treated with cyclosporin A // J. Rheumatol. – 1998. – Vol. 25, N 2. – P. 320-325.Szodoray P., Alex P., Chappell-Woodward C.M., Madland T.M., Knowlton N., Dozmorov I., Zeher M., Jarvis J.N., Nakken B., Brun J.G., Centola M. Circulating cytokines in Norwegian patients with psoriatic arthritis determined by a multiplex cytokine array system // Rheumatology (Oxford). – 2007. – Vol. 46, N 3. – P. 417-425.Szodoray P., Alex P., Chappell-Woodward C.M., Madland T.M., Knowlton N., Dozmorov I., Zeher M., Jarvis J.N., Nakken B., Brun J.G., Centola M. Circulating cytokines in Norwegian patients with psoriatic arthritis determined by a multiplex cytokine array system // Rheumatology (Oxford). – 2007. – Vol. 46, N 3. – P. 417-425.

The authors declare that there are no conflicts of interest present.