mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2018-5-739-746mimmun-1642Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSАНАЛИЗ ПОЛИМОРФНЫХ ВАРИАНТОВ ГЕНОВ ХЕМОКИНОВ, ИХ РЕЦЕПТОРОВ, ГЕНОВ БЕЛКОВ ОСТРОЙ ФАЗЫ ВОСПАЛЕНИЯ И ГЕНА CD4 ПРИ ФОРМИРОВАНИИ ОЖИРЕНИЯ У ЖЕНЩИНGENE POLYMORPHISM ANALYSIS OF CHEMOKINES, CHEMOKINE RECEPTORS, ACUTE PHASE PROTEINS, AND CD14 IN FEMALE OBESITY DEVELOPMENTКочетоваО. В.KochetovaO. V.

к.б.н., научный сотрудник.

450054, Россия, Республика Башкортостан, г. Уфа, пр. Октября, 71.

PhD (Biology), Research Associate.

Ufa.

Olga_MK78@mail.ruАхмадишинаЛ. З.AkhmadishinaL. Z.

к.б.н., научный сотрудник.

Уфа.

PhD (Biology), Research Associate.

Ufa.

КорытинаГ. Ф.KorytinaG. F.

д.б.н., старший научный сотрудник.

Уфа.

G.F., PhD, MD (Biology), Senior Research Associate.

ВиктороваТ. В.VictorovaТ. V.

д.м.н., главный научный сотрудник.

Уфа.

PhD, MD (Medicine), Main Research Associate.

ФГБУН «Институт биохимии и генетики Уфимского научного центра Российской академии наук».РоссияInstitute of Biochemistry and Genetics, Ufa Science Center.Russian Federation201805112018205739746Copyright © Кочетова О.В., Ахмадишина Л.З., Корытина Г.Ф., Викторова Т.В., 20182018Кочетова О.В., Ахмадишина Л.З., Корытина Г.Ф., Викторова Т.В.Kochetova O.V., Akhmadishina L.Z., Korytina G.F., Victorova Т.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1642

В настоящей работе была исследована частота генотипов и аллелей генов хемокинов (CXCL12 rs1801157, CCL2 rs1024611), рецепторов хемокинов (CCR5 del32, CX3CR1 rs3732378), гена белков острой фазы воспаления SAA rs1136743 и гена CD14 rs2569190 у женщин-татарок из Республики Башкортостан, страдающих ожирением и избыточной массой тела. Группу пациенток составили неродственные между собой женщины с ожирением ИМТ ≥ 30 кг/м2 (n = 225), женщины с избыточной массой тела ИМТ 25,0-29,9 кг/м2 (n = 184) и женщины контрольной группы (n = 327) ИМТ < 25,0 кг/м2. Генотипирование выполнено методом ПЦР-ПДРФ анализа. Группы пациенток и контроля отличались по таким показателям, как масса тела (р = 0,00001), уровень индекса массы тела (р = 0,001) и уровень глюкозы натощак (р = 0,0001). При проведении исследования была выявлена ассоциация с ожирением генотипов AG-AA (p = 0,007) и аллеля A (р = 0,003) полиморфного локуса rs3732378 гена CXCR1 и генотипа ТТ (p = 0,027) и аллели Т (р = 0,021) полиморфного локуса rs1136743 гена SAA. Показано, что генотип AA полиморфного локуса rs3732378 гена CX3CR1 ассоциирован с повышенной массой тела (р = 0,002) и повышенным уровнем ИМТ (р = 0,018); генотип GG полиморфного локуса rs1024611 гена CCL2 ассоциирован с повышенным уровнем глюкозы натощак (р = 0,001). На основе клинико-генетических данных и с использованием логистической регрессии выявлены статистически значимые различия, позволяющие прогнозировать развитие ожирения у женщин-татарок.

In the present study, we have investigated frequency of genotypes and functional alleles of genes encoding chemokines (CXCL12 rs1801157, CCL2 rs1024611), chemokine receptors (CCR5 del32, CX3CR1 rs3732378), acute phase proteins SAA rs1136743, and CD14 rs2569190 polymorphisms among Tatar obese or overweight women from the Republic of Bashkortostan.

The group of patients comprised unrelated women with obesity (BMI ≥ 30 kg/m2, n = 225), females with overweight (BMI 25.0-29.9 kg/m2, n = 184), and control group of women (n = 327) BMI < 25.0 kg/m2. Genotyping was performed by PCR-RFLP analysis. Patients and controls differed in such parameters as body weight (p = 0.00001), BMI level (p = 0.001) and fasting glucose level (p = 0.0001).

An association was revealed between obesity and AG-AA genotypes (p = 0.007) and A allele (p = 0.003) of polymorphic locus rs3732378 of CXCR1 gene, as well as TT genotype (p = 0.027) and T allele (p = 0.021) of polymorphic locus rs1136743 of SAA gene. It has been shown that the AA genotype of polymorphic locus rs3732378 of the CX3CR1 gene is associated with increased body weight (p = 0.002) and elevated BMI (p = 0.018); the GG genotype of polymorphic locus rs1024611 of the CCL2 gene is associated with elevated fasting glucose level (p = 0.001).

As based on clinical and genetic data and using logistic regression, some statistically significant differences were revealed, which allow to predict development of obesity in Tatar women.

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The authors declare that there are no conflicts of interest present.