mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2018-1-45-52mimmun-1431Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESЭФФЕКТЫ γc-ЦИТОКИНОВ (IL-2, IL-7 И IL-15) НА СОЗРЕВАНИЕ И ДИФФЕРЕНЦИРОВКУ CD45RО+CD4+/ CD8+Т-ЛИМФОЦИТОВ IN VITROEFFECTS OF γс-CYTOKINES (IL-2, IL-7 AND IL-15) UPON IN VITRO MATURATION AND DIFFERENTIATION OF CD4+CD45RО+/CD8+CD45RО+ T CELLSЮроваК. А.YurovaK. A.

к.м.н., научный сотрудник лаборатории иммунологии и клеточных биотехнологий ФГАОУ ВО «Балтийский федеральный университет имени И. Канта», г. Калининград

PhD (Medicine), Research Associate, Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad

larisalitvinova@yandex.ruХазиахматоваО. Г.KhaziakhmatovaO. G.

к.б.н., научный сотрудник лаборатории иммунологии и клеточных биотехнологий ФГАОУ ВО «Балтийский федеральный университет имени И. Канта», г. Калининград

PhD (Biology), Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad

larisalitvinova@yandex.ruТодосенкоН. М.TodosenkoN. M.

аспирант, младший научный сотрудник лаборатории иммунологии и клеточных биотехнологий ФГАОУ ВО «Балтийский федеральный университет имени И. Канта», г. Калининград

Junior Research Associate, Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad

larisalitvinova@yandex.ruЛитвиноваЛ. С.LitvinovaL. S.

д.м.н., заведующая лабораторией иммунологии и клеточных биотехнологий ФГАОУ ВО «Балтийский федеральный университет имени И. Канта», г. Калининград

PhD, MD (Medicine), Head, Laboratory of Immunology and Cell Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad

larisalitvinova@yandex.ruФГАОУ ВО «Балтийский федеральный университет имени И. Канта», г. КалининградРоссияImmanuel Kant Baltic Federal University, KaliningradRussian Federation2018100120182014552Copyright © Юрова К.А., Хазиахматова О.Г., Тодосенко Н.М., Литвинова Л.С., 20182018Юрова К.А., Хазиахматова О.Г., Тодосенко Н.М., Литвинова Л.С.Yurova K.A., Khaziakhmatova O.G., Todosenko N.M., Litvinova L.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1431

Исследовано влияние γс-цитокинов (IL-2, IL-7 и IL-15) на созревание и дифференцировку цитотоксических и хелперных популяций CD45RО+Т-клеток в условиях гомеостатического культивирования in vitro. Выявлено, что IL-2, IL-7 и IL-15 опосредуют созревание и дифференцировку CD8+Т-лимфоцитов центральной памяти, пополняя популяцию клеток, обладающих эффекторными функциями. Действие IL-2 на CD4+Т-клетки центральной памяти сопровождается генерацией клеток эффекторной памяти за счет снижения числа незрелых эффекторов – CD62L–CD27+, тогда как IL-7 стимулирует образование незрелых эффекторов – CD62L–CD27+. В субпопуляции CD8+CD45RО+ лимфоцитовIL-2, IL-7 и IL-15 инициируют образование терминально-дифференцированных клеток, обеспечивающих устойчивую иммунологическую память на реинвазию патогена.

 

Effect of γс-cytokines (IL-2, IL-7 и IL-15) upon maturation and differentiation of cytotoxic and helper CD45RО+ Tcell population was studied in the homeostatic in vitro culture conditions. We have found that IL-2, IL-7 and IL-15 mediate maturation and differentiation of CD8 T cells central memory, replenishing the population of cells that exhibit effector functions. Action of IL-2 on CD4+ central memory T cells is accociated with generation of effector memory cells by reducing the number of immature effectors (CD62L–CD27+), whereas IL-7 promotes the formation of immature (CD62L–CD27+) effectors. IL-2, IL-7 and IL-15 initiate formation of terminally-differentiated cells in a subpopulation of CD8+CD45RO+ lymphocytes, providing a stable immunological memory to a pathogen reinfestation.

 

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The authors declare that there are no conflicts of interest present.