mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2017-3-285-292mimmun-1262Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВЛИЯНИЕ ФАКТОРОВ, СЕКРЕТИРУЕМЫХ ПЛАЦЕНТОЙ, НА ФОРМИРОВАНИЕ СОСУДОПОДОБНЫХ СТРУКТУР ЭНДОТЕЛИАЛЬНЫМИ КЛЕТКАМИ В ПРИСУТСТВИИ КЛЕТОК ТРОФОБЛАСТАEFFECTS OF PLACENTAL FACTORS UPON DEVELOPMENT OF TUBULAR STRUCTURES BY ENDOTHELIAL CELLS IN PRESENCE OF TROPHOBLASTIC CELLSСоколовД. И.SokolovD. I.

д.б.н., заведующий лабораторией межклеточных взаимодействий, отдел иммунологии и межклеточных взаимодействий ФГБНУ «Научно- исследовательский институт акушерства, гинекологии и репродуктологии имени Д.О. Отта»; ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения РФ, кафедра иммунологии, Санкт-Петербург, Россия

PhD, MD (Biology), Head, Laboratory of Cell Interactions, Department of Immunology and Cell Interactions, D. Ott Research Institute of Obstetrics, Ginecology and Reproductology; Pavlov First St. Petersburg State Medical University, Department оf Immunology, St. Petersburg

falcojugger@yandex.ruБеляковаК. Л.BelyakovaK. L.

научный сотрудник межклеточных взаимодействий, отдел иммунологии и межклеточных взаимодействий

Research Associate, Laboratory of Cell Interactions, Department of Immunology and Cell Interactions

falcojugger@yandex.ruМихайловаВ. А.MikhailovaV. A.

к.б.н., научный сотрудник лаборатории межклеточных взаимодействий, отдел иммунологии и межклеточных взаимодействий ФГБНУ «Научно-исследовательский институт акушерства, гинекологии и репродуктологии имени Д.О. Отта»; ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения РФ, кафедра иммунологии, Санкт- Петербург

PhD (Biology), Research Associate, Laboratory of Cell Interactions, Department of Immunology and Cell Interactions, D. Ott Research Institute of Obstetrics, Ginecology and Reproductology; Pavlov First St. Petersburg State Medical University, Department оf Immunology, St. Petersburg

falcojugger@yandex.ruШиленковаЮ. В.ShilenkovaYu. V.

клинический ординатор отделения оперативной гинекологии

Clinical Resident, Department of Surgical Gynecology

falcojugger@yandex.ruХудинянМ. М.KhudinyanM. M.

лаборант-исследователь лаборатории межклеточных взаимодействий, отдел иммунологии и межклеточных взаимодействий

Laboratory Research Assistant, Laboratory of Cell Interactions, Department of Immunology and Cell Interactions

falcojugger@yandex.ruОкороковаЛ. С.OkorokovaK. S.

лаборант-исследователь лаборатории межклеточных взаимодействий, отдел иммунологии и межклеточных взаимодействий

Laboratory Research Assistant, Laboratory of Cell Interactions, Department of Immunology and Cell Interactions

falcojugger@yandex.ruБеликоваМ. Е.BelikovaM. E.

к.м.н., заведующая гинекологическим отделением

PhD (Medicine), Head, Department of Gynecology

falcojugger@yandex.ruСельковС. А.SelkovS. A.

д.м.н., профессор, руководитель отдела иммунологии и межклеточных взаимодействий

PhD, MD (Medicine), Professor, Head, Department of Immunology and Cell Interactions

falcojugger@yandex.ruФГБНУ «Научно-исследовательский институт акушерства, гинекологии и репродуктологии имени Д.О. Отта», Санкт-Петербург ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения РФ, Санкт-ПетербургРоссияD. Ott Research Institute of Obstetrics, Ginecology and Reproductology, St. Petersburg Pavlov First St. Petersburg State Medical University, St. PetersburgRussian FederationФГБНУ «Научно-исследовательский институт акушерства, гинекологии и репродуктологии имени Д.О. Отта», Санкт-ПетербургРоссияD. Ott Research Institute of Obstetrics, Ginecology and Reproductology, St. PetersburgRussian FederationСПб ГБУЗ «Городская больница № 26, Санкт-ПетербургРоссияSt. Petersburg City Hospital No. 26, St. PetersburgRussian Federation201704072017193285292Copyright © Соколов Д.И., Белякова К.Л., Михайлова В.А., Шиленкова Ю.В., Худинян М.М., Окорокова Л.С., Беликова М.Е., Сельков С.А., 20172017Соколов Д.И., Белякова К.Л., Михайлова В.А., Шиленкова Ю.В., Худинян М.М., Окорокова Л.С., Беликова М.Е., Сельков С.А.Sokolov D.I., Belyakova K.L., Mikhailova V.A., Shilenkova Y.V., Khudinyan M.M., Okorokova K.S., Belikova M.E., Selkov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1262

Клетки трофобласта активно взаимодействуют с эндотелиальными клетками, участвуя в формировании сосудов в зоне маточно-плацентарного контакта и ремоделировании спиральных артерий матки. Продукция цитокинов клетками плаценты закономерно изменяется от первого к третьему триместру физиологической беременности; она также изменяется в зависимости от наличия акушерской патологии, например при преэклампсии. В настоящее время нет данных о влиянии как отдельных цитокинов, так и факторов, секретируемых плацентой, на взаимодействие ЭК и трофобласта. Поэтому целью настоящего исследования явилась оценка факторов, секретируемых плацентой, на формирование сосудоподобных структур эндотелиальными клетками в присутствии трофобласта. Проводили совместное культивирование эндотелиальных клеток линии EA.Hy926 и клеток трофобласта линии JEG-3 на трехмерном коллагеновом матриксе «Matrigel» (BD, США) в присутствии факторов, секретируемых плацентой женщин с физиологической беременностью на сроке 9-11 недель (n = 15), женщин с физиологической беременностью на сроке 38-39 недель (n = 15), женщин с беременностью, осложненной преэклампсией на сроке 38-39 недель (n = 14). Установлено, что клетки трофобласта модифицируют способность эндотелиальных клеток образовывать сосуды только в условиях физиологически протекающей беременности. При преэклампсии клетки трофобласта не способны скорректировать поведение эндотелиальных клеток и обеспечить физиологический рост сосудов.

Trophoblast cells actively interact with endothelial cells participating in the process of vasculogenesis in the uterus/placenta contact area and remodeling of uterine spiral arteries. Cytokine production by the placental cells is subject to gradual changes from the 1st to 3rd trimester of physiological pregnancy. It is also changed in cases of obstetric disorders, e.g., in pre-eclampsia. At present time, there are lacking data on effects of cytokines and placenta-derived factors upon local interactions between endothelium and trophoblast cells. Hence, the aim of our study was to assess the influence of placental factors upon formation of tube-like structures by endothelial cells in presence of trophoblastic cells. We performed co-cultures of Ea.Hy926 endothelial cell line and Jeg-3 trophoblastic cells in a 3-D collagen matrix («Matrigel», BD, USA) with secretable factors from placentas of healthy pregnant women at 9-11 weeks of gestation (n = 15), healthy pregnant women at 38-39 weeks of gestation (n = 15), or the women with preeclampsia at 38-39 weeks of gestation (n = 14). We have shown that the trophoblastic cells may modify the ability of endothelial cells to form tube-like structures only with placental factors from health pregnant women. In pre-eclampsia condition, the trophoblast cells are not able to correct the behavior of endothelial cells, and to promote physiological growth of blood vessels.

 

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The authors declare that there are no conflicts of interest present.