mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2016-5-475-482mimmun-1073Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSОСОБЕННОСТИ ЦИТОКИНОВОЙ РЕГУЛЯЦИИ РЕСПИРАТОРНОГО ВЗРЫВА НЕЙТРОФИЛОВ КРОВИ В ПРОГНОЗЕ РАЗВИТИЯ АБДОМИНАЛЬНОГО СЕПСИСА У БОЛЬНЫХ РАСПРОСТРАНЕННЫМ ГНОЙНЫМ ПЕРИТОНИТОМCYTOKINE REGULATION OF RESPIRATORY BURST IN BLOOD NEUTROPHILS FOR PREDICTION OF ABDOMINAL SEPSIS IN PATIENTS WITH EXTENDED PURULENT PERITONITISСавченкоА. А.SavchenkoA. A.

д.м.н., профессор, руководитель лаборатории молекулярно-клеточной физиологии и патологии,

660022, г. Красноярск, ул. Партизана Железняка, 3г

PhD, MD (Medicine), Professor, Head, Laboratory of Molecular and Cellular Physiology and Pathology,

660022, Krasnoyarsk, Partizan Zeleznyak str., 3g

aasavchenko@yandex.ruБорисовА. Г.BorisovA. G.

к.м.н., ведущий научный сотрудник лаборатории молекулярно-клеточной физиологии и патологии,

 г. Красноярск

PhD (Medicine), Leading Research Associate, Laboratory of Molecular and Cellular Physiology and Pathology,

Krasnoyarsk

ЗдзитовецкийД. Э.ZdzitovetskyD. E.

д.м.н., профессор, заведующий кафедрой и клиникой хирургических болезней им. проф. Ю.М. Лубенского,

г. Красноярск

PhD, MD (Medicine), Professor, Head of Department and Clinic of Surgical Illnesses it. J.M. Lubinskogo,

Krasnoyarsk

ГвоздевИ. И.GvozdevI. I.

младший научный сотрудник лаборатории клеточно-молекулярной физиологии и патологии,

г. Красноярск

Junior Research Associate, Laboratory of Molecular and Cellular Physiology and Pathology,

Krasnoyarsk

ФГБНУ «Научно-исследовательский институт медицинских проблем Севера»; ГБОУ ВПО «Красноярский государственный медицинский университет им. проф. В.Ф. Войно-Ясенецкого» Министерства здравоохранения РФРоссияResearch Institute of Medical Problems of the North; Krasnoyarsk State V.F. Voino-Yasenetsky Medical UniversityRussian FederationФГБНУ «Научно-исследовательский институт медицинских проблем Севера», г. Красноярск ГБОУ ВПО «Красноярский государственный медицинский университет им. проф. В.Ф. Войно-Ясенецкого» Министерства здравоохранения РФРоссияResearch Institute of Medical Problems of the North; Krasnoyarsk State V.F. Voino-Yasenetsky Medical UniversityRussian FederationГБОУ ВПО «Красноярский государственный медицинский университет им. проф. В.Ф. Войно-Ясенецкого» Министерства здравоохранения РФРоссияKrasnoyarsk State V.F. Voino-Yasenetsky Medical UniversityRussian FederationФГБНУ «Научно-исследовательский институт медицинских проблем Севера»РоссияResearch Institute of Medical Problems of the NorthRussian Federation201625082016185475482Copyright © Савченко А.А., Борисов А.Г., Здзитовецкий Д.Э., Гвоздев И.И., 20162016Савченко А.А., Борисов А.Г., Здзитовецкий Д.Э., Гвоздев И.И.Savchenko A.A., Borisov A.G., Zdzitovetsky D.E., Gvozdev I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1073

The aim of this study was to evaluate correlations between cytokine concentrations and parameters of respiratory burst in blood neutrophils, in order to predict potential development of abdominal sepsis in the patients with extended purulent peritonitis (EPP). The study involved fifteen patients with EPP. Peripheral blood samples were taken on the first day post-admissiona. Abdominal sepsis was diagnosed in thirty-five patients (70%) within 5…10 days after surgical intervention. Clinical complications were absent in fifteen patients (30%). Sixty-seven healthy individuals were examined as a control group. Evaluation of the cytokine concentrations was performed by ELISA technique. The degree of respiratory burst in blood neutrophils was measured by means of chemiluminescence assay.

It is revealed that the EPP patients exhibited increased levels of serum proinflammatory cytokines and IFNγ, along with higher intensity of respiratory burst in blood neutrophils, due to activated synthesis of of both primary and secondary reactive oxygen species (ROS). The EPP patients who later developed abdominal sepsis showed reduced spontaneous synthesis of primary ROS and increased spontaneous synthesis of secondary ROS. Upon zymosan induction of neutrophils, both primary and secondary ROS levels proved to be similar in the EPP subgroups with or without subsequent sepsis. EPP patients with uncomplicated post-surgical period still exhibited a predominant regulation of respiratory burst of neutrophils by IFNγ activity. Meanwhile, the neutrophil respiratory burst was correlated with TNFα and IL-6 in those patients who further developed abdominal sepsis. A stimulatory effect of IFNγ and a presumably inhibitory action of TNFα and IL-6 upon respiratory burst of blood neutrophils in EPP patients are associated with a release of large cytokine amounts during acute immune inflammatory events, and migration of activated cells to the inflammatory focus. In particular, the changes in the cytokine levels regulating the respiratory burst of neutrophils may be an important factor which predicts development of abdominal sepsis in EPP.

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The authors declare that there are no conflicts of interest present.