mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2016-5-437-442mimmun-1067Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESОСОБЕННОСТИ СЕКРЕЦИИ ХЕМОКИНОВ МОНОНУКЛЕАРНЫМИ И ДЕНДРИТНЫМИ КЛЕТКАМИ: РОЛЬ ГИСТАМИНОВЫХ РЕЦЕПТОРОВ Н3/Н4-ТИПАCHEMOKINE SECRETION PATTERNS IN MONONUCLEAR AND DENDRITIC CELLS: ROLE OF HISTAMINE TYPE H3/H4 RECEPTORSЕвстратоваВ. С.EvstratovaV. S.

младший научный сотрудник лаборатории иммунологии,

Москва

Junior Research Associate, Laboratory of Immunology,

Moscow

РигерН. А.RigerN. A.

д.м.н., старший научный сотрудник лаборатории иммунологии,

Москва

PhD, MD (Medicine), Senior Research Associate, Laboratory of Immunology,

Moscow

НикитюкД. Б.NikityukD. B.

д.м.н., профессор, врио директора,

Москва

PhD, MD (Medicine), Professor, Acting Director,

Moscow

ХанферьянР. А.KhanferyanR. A.

д.м.н., профессор, заведующий лабораторией иммунологии,

109240, Москва, Устьинский проезд, 2/14

PhD, MD (Medicine), Professor, Head, Laboratory of Immunology,

109240, Moscow, Ustyinsky pas., 2/14

khanferyan@ion.ruФГБУН «Федеральный исследовательский центр питания, биотехнологии и безопасности пищи»РоссияFederal Research Center of Nutrition and BiotechnologyRussian Federation201624082016185437442Copyright © Евстратова В.С., Ригер Н.А., Никитюк Д.Б., Ханферьян Р.А., 20162016Евстратова В.С., Ригер Н.А., Никитюк Д.Б., Ханферьян Р.А.Evstratova V.S., Riger N.A., Nikityuk D.B., Khanferyan R.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1067

В работе представлены результаты исследования влияния антагониста Н3/Н4 гистаминовых рецепторов (Ципроксифан) на секреторную активность дендритных клеток (ДК) и мононуклеарных клеток периферической крови (МПК). Установлено, что блокада гистаминовых рецепторов Н3/Н4-типа in vitro различно влияет на синтез основных хемокинов (Eotaxin, RANTES, MCP-1, MIP-1α, MIP-1β, GRO-α, IL-8) МПК и ДК, выращенными из них. Так, в культуре ДК секреция RANTES, GRO-α снижалась на 20 и 40% соответственно, в то время как секреция MIP-1β практически не изменялась, а секреция MCP-1 в 4 раза увеличивалась. В культурах МПК же достоверно усиливалась секреция Eotaxin, RANTES, MIP-1α, MIP-1β, GRO-α, IL-8 (p < 0,05).

The study presents results concerning effects of H3/H4 histamine receptors antagonist (Ciproxifan) upon in vitro chemokine secretion by dendritic cells (DC) and peripheral blood mononuclear cells (PBMC). We have been shown that in vitro inhibition of type H3/H4 histamine receptor affects in different ways production of major chemokines by PBMC and DC, including eotaxin, RANTES, MCP-1, MIP-1α, MIP-1β, GRO-α, and IL-8. E.g., RANTES and GRO-α secretion in DC cultures was reduced, respectively, by 20% and 40%. Meanwhile, MIP-1β secretion was virtually unchanged, whereas MCP-1 secretion exhibited a four-fold increase. In PBMC cultures, the H3/H4 receptor antagonist induced a significant increase in eotaxin, RANTES, MIP-1α, MIP-1β, GRO-α, and IL-8 secretion (p < 0.05).

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The authors declare that there are no conflicts of interest present.